@article{mbs:/content/journal/jgv/10.1099/vir.0.18845-0, author = "Mahot, Segolene and Sergeant, Alain and Drouet, Emmanuel and Gruffat, Henri", title = "A novel function for the Epstein–Barr virus transcription factor EB1/Zta: induction of transcription of the hIL-10 gene", journal= "Journal of General Virology", year = "2003", volume = "84", number = "4", pages = "965-974", doi = "https://doi.org/10.1099/vir.0.18845-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.18845-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Interleukin-10 (IL-10) plays a critical role in Epstein–Barr virus (EBV) biology. Indeed, the EBV genome contains a gene (BCRF1) with homology to the human IL-10 (hIL-10) gene. In addition to viral IL-10, which is secreted late in the productive cycle, hIL-10 production is also induced in B cells infected by EBV. The EBV protein LMP-1 and the viral small non-polyadenylated RNAs (EBERs) expressed during latency are involved in hIL-10 induction. In this study, we show that in B cells the viral transcription factor EB1, which is the main inducer of the EBV productive cycle, also activates transcription of the hIL-10 gene and secretion of the hIL-10 protein. Accordingly, EB1 bound directly to specific DNA sequences in the hIL-10 minimal promoter. Moreover, specific disruption of EB1 binding to some of these sites impaired EB1-mediated activation of transcription at the hIL-10 promoter in a transient expression assay. Therefore, an increase in IL-10 production occurs during latency and early and late during the productive cycle. This production of IL-10 might favour the survival of EBV-infected cells in vivo and/or create a microenvironment required for efficient de novo infection of B lymphocytes by EBV virions.", }