1887

Abstract

Ld652Y cells derived from the gypsy moth, , were infected with seven different nucleopolyhedroviruses (NPVs) including those from , (BmNPV), (HycuNPV), (SeMNPV), , (OpMNPV) and (SpltMNPV). The results showed that Ld652Y cells infected with BmNPV, HycuNPV, SeMNPV, OpMNPV and SpltMNPV underwent apoptosis, displaying apoptotic bodies, characteristic DNA fragmentation and increased caspase-3-like protease activity; HycuNPV induced the most severe apoptosis. In HycuNPV-infected Ld652Y cells, a considerable amount of viral DNA was synthesized although there was no detectable yield of budded virions and polyhedrin. Northern blot and immunoblot analyses revealed that HycuNPV inhibitor of apoptosis 3 (IAP3), which has been shown to function in Sf9 cells, was expressed in HycuNPV-infected Ld652Y cells at a level higher than or comparable with that in HycuNPV-infected SpIm cells, which produced a high titre of progeny virions without any apoptotic response. These results imply that the relative ease of apoptosis induction in NPV-infected Ld652Y cells is largely dependent on inherent cellular properties rather than functions of the respective NPVs, and indicate that the defect in progeny virion production is not merely due to the virus-induced apoptosis in HycuNPV-infected Ld652Y cells.

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2003-03-01
2020-09-29
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References

  1. Adams J. R., McClintock J. T.. 1991; Baculoviridae. Nuclear polyhedrosis viruses. Part 1. Nuclear polyhedrosis viruses of insects. In Atlas of Invertebrate Viruses pp 89–204 Edited by Adams J. R., Bonami J. R.. Boca Raton: CRC Press;
    [Google Scholar]
  2. Ahmad M., Srinivasula S. M., Wang L., Litwack G., Fernandes-Alnemri T., Alnemri E. S.. 1997; Spodoptera frugiperda caspase-1, a novel insect death protease that cleaves the nuclear immunophilin FKBP46, is the target of the baculovirus antiapoptotic protein p35. J Biol Chem272:1421–1424
    [Google Scholar]
  3. Bertin J., Mendrysa S. M., LaCount D. J., Gaur S., Krebs J. F., Armstrong R. C., Tomaselli K. J., Friesen P. D.. 1996; Apoptotic suppression by baculovirus P35 involves cleavage by and inhibition of a virus-induced CDE-3/ICE-like protease. J Virol70:6251–6259
    [Google Scholar]
  4. Blissard G., Black B., Crook N., Keddie B., Possee R., Rohrmann G., Theilmann D., Volkman L.. 2000; Family Baculoviridae . In Virus Taxonomy . Seventh Report of the International Committee on Taxonomy of Viruses . pp 195–202 Edited by van Regenmortel M. H. V., Fauquet C. M., Bishop D. H. L., Carstens E. B., Estes M. K., Lemon S. M., Maniloff J., Mayo M. A., McGeoch D. J., Pringle C. R., Wickner R. B.. San Diego: Academic Press;
  5. Bradford M. B., Blissard G. W., Rohrmann G. F.. 1990; Characterization of the infection cycle of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus in Lymantria dispar cells. J Gen Virol71:2841–2846
    [Google Scholar]
  6. Carpenter W. M., Bilimoria S. L.. 1983; A semipermissive nuclear polyhedrosis virus infection: characterization of infection kinetics and morphogenesis. Virology130:227–231
    [Google Scholar]
  7. Chejanovsky N., Gershburg E.. 1995; The wild-type Autographa californica nuclear polyhedrosis virus induces apoptosis of Spodoptera littoralis cells. Virology209:519–525
    [Google Scholar]
  8. Chen C.-J., Quentin M. E., Brennan L. A., Kukel C., Thiem S. M.. 1998; Lymantria dispar nucleopolyhedrovirus hrf-1 expands the larval host range of Autographa californica nucleopolyhedrovirus. J Virol72:2526–2531
    [Google Scholar]
  9. Clem R. J.. 1997; Regulation of programmed cell death by baculoviruses. In The Baculoviruses pp 237–266 Edited by Miller L. K.. New York: Plenum Press;
    [Google Scholar]
  10. Clem R. J.. 2001; Baculoviruses and apoptosis: the good, the bad, and the ugly. Cell Death Differ8:137–143
    [Google Scholar]
  11. Clem R. J., Miller L. K.. 1993; Apoptosis reduces both the in vitro replication and the in vivo infectivity of a baculovirus. J Virol67:3730–3738
    [Google Scholar]
  12. Clem R. J., Fechheimer M., Miller L. K.. 1991; Prevention of apoptosis by a baculovirus gene during infection of insect cells. Science254:1388–1390
    [Google Scholar]
  13. Dai X., Shi X., Pang Y., Su D.. 1999; Prevention of baculovirus-induced apoptosis of BTI-Tn-5B1–4 (Hi5) cells by the p35 gene of Trichoplusia ni multicapsid nucleopolyhedrovirus. J Gen Virol80:1841–1845
    [Google Scholar]
  14. Du X., Thiem S. M.. 1997; Responses of insect cells to baculovirus infection: protein synthesis shutdown and apoptosis. J Virol71:7866–7872
    [Google Scholar]
  15. Gershburg E., Rivkin H., Chejanovsky N.. 1997; Expression of the Autographa californica nuclear polyhedrosis virus apoptotic suppressor gene p35 in nonpermissive Spodoptera littoralis cells. J Virol71:7593–7599
    [Google Scholar]
  16. Goodwin R. H., Tompkins G. J., McCawley P.. 1978; Gypsy moth cell lines divergent in viral susceptibility. I. Culture and identification. In Vitro (Rockv14485–494
    [Google Scholar]
  17. Guzo D., Dougherty E. M., Lynn D. E., Braun S. K., Weiner R. M.. 1991; Changes in macromolecular synthesis of gypsy moth cell line IPLB-Ld652Y induced by Autographa californica nuclear polyhedrosis virus infection. J Gen Virol72:1021–1029
    [Google Scholar]
  18. Guzo D., Rathburn H., Guthrie K., Dougherty E.. 1992; Viral and host cellular transcription in Autographa californica nuclear polyhedrosis virus-infected gypsy moth cell line. J Virol66:2966–2972
    [Google Scholar]
  19. Hara K., Funakoshi M., Kawarabata T.. 1995; A cloned cell line of Spodoptera exigua has a highly increased susceptibility to the Spodoptera exigua nuclear polyhedrosis virus. Can J Microbiol41:1111–1116
    [Google Scholar]
  20. Hershberger P. A., Dickson J. A., Friesen P. D.. 1992; Site-specific mutagenesis of the 35-kilodalton protein gene encoded by Autographa californica nuclear polyhedrosis virus: cell line-specific effects on virus replication. J Virol66:5525–5533
    [Google Scholar]
  21. Hink W. F.. 1970; Established insect cell line from the cabbage looper, Trichoplusia ni . Nature226:466–467
    [Google Scholar]
  22. Ikeda M., Kobayashi M.. 1999; Cell-cycle perturbation in Sf9 cells infected with Autographa californica nucleopolyhedrovirus. Virology258:176–188
    [Google Scholar]
  23. Ikeda M., Katou Y., Yamada Y., Chaeychomsri S., Kobayashi M.. 2001; Characterization of Autographa californica nucleopolyhedrovirus infection in cell lines from Bombyx mori . J Insect Biotechnol Sericol70:49–58
    [Google Scholar]
  24. Katou Y., Ikeda M., Kobayashi M.. 2001; Characterization of Bombyx mor i nucleopolyhedrovirus infection of Spodoptera frugiperda cells. J Insect Biotechnol Sericol70:137–147
    [Google Scholar]
  25. Kobayashi M., Kotake M., Sugimori H., Nagamine T., Kajiura Z.. 1990; Identification of virus-specific polypeptides and translatable mRNAs in the isolated pupal abdomens of the silkworm, Bombyx mori , infected with nuclear polyhedrosis virus. J Invertebr Pathol55:52–60
    [Google Scholar]
  26. LaCount D. J., Friesen P. D.. 1997; Role of early and late replication events in induction of apoptosis by baculoviruses. J Virol71:1530–1537
    [Google Scholar]
  27. LaCount D. J., Hanson S. F., Schneider C. L., Friesen P. D.. 2000; Caspase inhibitor P35 and inhibitor of apoptosis Op-IAP block in vivo proteolytic activation of an effector caspase at different steps. J Biol Chem275:15657–15664
    [Google Scholar]
  28. Laviña B. A., Padua L. E., Wu F. Q., Shirata N., Ikeda M., Kobayashi M.. 2001; Biological characterization of a nucleopolyhedrovirus of Spodoptera litura (Lepidoptera: Noctuidae) isolated from the Philippines. Biol Control20:39–47
    [Google Scholar]
  29. Laviña-Caoili B. A., Kamiya K., Kawamura S., Ikeda M., Kobayashi M.. 2001; Comparative in vitro analysis of geographic variants of nucleopolyhedrovirus of Spodoptera litura isolated from China and the Philippines. J Insect Biotechnol Sericol70:199–209
    [Google Scholar]
  30. Leisy D. J., Rohrmann G. F., Nesson M., Beaudreau G. S.. 1986; Nucleotide sequencing and transcriptional mapping of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus p10 gene. Virology153:157–167
    [Google Scholar]
  31. Liu H.-S., Bilimoria S. L.. 1990; Infected cell specific protein and viral DNA synthesis in productive and abortive infections of Spodoptera frugiperda nuclear polyhedrosis virus. Arch Virol115:101–113
    [Google Scholar]
  32. Liu J. J., Carstens E. B.. 1993; Infection of Spodoptera frugiperda and Choristoneura fumiferana cell lines with the baculovirus Choristoneura fumiferana nuclear polyhedrosis virus. Can J Microbiol39:932–940
    [Google Scholar]
  33. Maeda S.. 1989; Gene transfer vector of a baculovirus, Bombyx mori , and their use for expression of foreign genes in insect cells. In Invertebrate Cell System Applications pp 167–181 Edited by Mitsuhashi J.. Boca Raton: CRC Press;
    [Google Scholar]
  34. Manji G. A., Friesen P. D.. 2001; Apoptosis in motion. An apical, p35 -insensitive caspase mediates programmed cell death in insect cells. J Biol Chem276:16704–16710
    [Google Scholar]
  35. Mazzacano C. A., Du X., Thiem S. M.. 1999; Global protein synthesis shutdown in Autographa californica nucleopolyhedrovirus-infected Ld652Y cells is rescued by tRNA from uninfected cells. Virology260:222–231
    [Google Scholar]
  36. Mitsuhashi J.. 1995; A continuous cell line from pupal ovaries of the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae). Appl Entomol Zool30:75–82
    [Google Scholar]
  37. Mitsuhashi J., Inoue H.. 1988; Obtainment of a continuous cell line from the larval fat bodies of the mulberry tiger moth, Spilosoma imparilis (Lepidoptera: Arctiidae). Appl Entomol Zool23:488–490
    [Google Scholar]
  38. Mitsuhashi J., Maramorosch K.. 1964; Leafhopper tissue culture: embryonic, nymphal, and imaginal tissues from aseptic insects. Contrib Boyce Thompson Inst22:435–460
    [Google Scholar]
  39. Morris T. D., Miller L. K.. 1993; Characterization of productive and non-productive AcMNPV infection in selected insect cell lines. Virology197:339–348
    [Google Scholar]
  40. Nagamine T., Shimomura M., Sugimori H., Kobayashi M.. 1989; Titration of Bombyx mori (Lepidoptera: Bombycidae) nuclear polyhedrosis virus in a Bombyx mori cell line. Appl Entomol Zool24:235–237
    [Google Scholar]
  41. Palli S. R., Caputo G. F., Sohi S. S., Brownwright A. J., Ladd T. R., Cook B. J., Primavera M., Arif B. M., Retnakaran A.. 1996a; Cf MNPV blocks Ac MNPV-induced apoptosis in a continuous midgut cell line. Virology222:201–213
    [Google Scholar]
  42. Palli S. R., Sohi S. S., Cook B. J., Brownwright A. J., Caputo G. F., Retnakaran A.. 1996b; RNA- and protein-synthesis inhibitors induce apoptosis in a midgut cell line from the spruce budworm, Choristoneura fumiferana . J Insect Physiol42:1061–1069
    [Google Scholar]
  43. Prikhod'ko E. A., Miller L. K.. 1996; Induction of apoptosis by baculovirus transactivator IE1. J Virol70:7116–7124
    [Google Scholar]
  44. Prikhod'ko E. A., Miller L. K.. 1999; The baculovirus PE38 protein augments apoptosis induced by transactivator IE1. J Virol73:6691–6699
    [Google Scholar]
  45. Seshagiri S., Miller L. K.. 1997; Baculovirus inhibitors of apoptosis (IAPs) block activation of Sf-caspase-1. Proc Natl Acad Sci U S A94:13606–13611
    [Google Scholar]
  46. Shirata N., Ikeda M., Kamiya K., Kawamura S., Kunimi Y., Kobayashi M.. 1999; Replication of nucleopolyhedroviruses of Autographa californica (Lepidoptera: Noctuidae), Bombyx mori (Lepidoptera: Bombycidae), Hyphantria cunea (Lepidoptera: Arctiidae), and Spodoptera exigua (Lepidoptera: Noctuidae) in four lepidopteran cell lines. Appl Entomol Zool 34:507–516
    [Google Scholar]
  47. Slavicek J. M., Podgwaite J., Lanner-Herrera C.. 1992; Properties of two Lymantria dispar nuclear polyhedrosis virus isolates obtained from the microbial pesticide Gypchek. J Invertebr Pathol59:142–148
    [Google Scholar]
  48. Slavicek J. M., Mercer M. J., Kelly M. E., Hayes-Plazolles H.. 1996; Isolation of a baculovirus variant that exhibits enhanced polyhedra production stability during serial passage in cell culture. J Invertebr Pathol67:153–160
    [Google Scholar]
  49. Smith G. E., Summers M. D.. 1978; Analysis of baculovirus genomes with restriction endonucleases. Virology89:517–527
    [Google Scholar]
  50. Thiem S. M., Du X., Quentin M. E., Berner M. M.. 1996; Identification of a baculovirus gene that promotes Autographa californica nuclear polyhedrosis virus replication in a nonpermissive insect cell line. J Virol70:2221–2229
    [Google Scholar]
  51. Tsuda K., Mizuki E., Kawarabata T., Aizawa K.. 1984; Replication of Xestia c-nigrum (Lepidoptera: Noctuidae) nuclear polyhedrosis virus in continuous cell cultures. Appl Entomol Zool19:293–298
    [Google Scholar]
  52. Wu F. Q., Laviña B. A., Ikeda M., Shirata N., Cai Y. X., Pan S. X., Kobayashi M.. 2000; Cloning and biological characterization of Spodoptera exigua nucleopolyhedroviruses isolated in China. J Seric Sci Jpn69:177–189
    [Google Scholar]
  53. Yanase T., Yasunaga C., Kawarabata T.. 1998; Replication of Spodoptera exigua nucleopolyhedrovirus in permissive and non-permissive lepidopteran cell lines. Acta Virol42:293–298
    [Google Scholar]
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