1887

Abstract

Full-length genomic cDNA clones of the Swedish isolate of (PMTV) were transcribed using T7 RNA polymerase. The combination of RNA 1, 2 and 3 synthesized in the presence of mGpppG cap analogue was infectious when inoculated onto plants. Also, the combination of RNA 1 (encodes the viral replicase) with RNA 3 [encodes the triple gene block proteins and a small cysteine-rich protein (CRP)] was infectious and both RNAs moved systemically in . plants in the absence of RNA 2, which encodes the coat protein (CP). However, the yellow mosaic symptoms that typically developed following PMTV infection with all three RNAs were not observed in plants infected with RNA 1+RNA 3. Site-directed mutagenesis experiments revealed that expression of the putative CRP was not required for systemic infection and symptom induction in . . These data show that PMTV represents an example of a multipartite virus capable of establishing systemic infection without the CP-encoding RNA, and also without the putative CRP.

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2003-04-01
2019-10-22
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