1887

Abstract

Studies to test the transmissibility of the bovine spongiform encephalopathy (BSE) agent to pigs began in 1989. Parenteral inoculation of the agent by three routes simultaneously (intracranially, intravenously and intraperitoneally) produced disease with an incubation period range of 69–150 weeks. Pre-clinical pathological changes were detected in two pigs killed electively at 105 and 106 weeks post-inoculation. Infectivity was detected by bioassay in inbred mice in the CNS of those pigs that developed spongiform encephalopathy. Infectivity was also found in the stomach, jejunum, distal ileum and pancreas of terminally affected pigs. These findings show that pigs are susceptible to BSE. In contrast, disease failed to occur in pigs retained for 7 years after exposure by feeding BSE-affected brain on three separate days, at 1–2 week intervals. The amounts fed each day were equivalent to the maximum daily intake of meat and bone meal in rations for pigs aged 8 weeks. No infectivity was found in tissues assayed from the pigs exposed orally. This included tissues of the alimentary tract. It is suggested that these pigs did not become infected. The relatively high oral exposure used in these experiments compared with feed-borne exposure in the field may explain the absence of an epidemic of spongiform encephalopathy in domestic pigs concurrent with the BSE epidemic in the UK.

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2003-04-01
2021-10-28
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References

  1. Anon. 1988 The Bovine Spongiform Encephalopathy Order 1988 . Statutory Instrument 1988, No. 1039 London: HMSO;
    [Google Scholar]
  2. Anon. 1996; The Bovine Spongiform Encephalopathy (Amendment) Order 1996 . Statutory Instrument, 1996. No. 962 London: HMSO;
    [Google Scholar]
  3. Baker H. F., Ridley R. M., Wells G. A. H. 1993; Experimental transmission of BSE and scrapie to the common marmoset. Vet Rec 132:403–406
    [Google Scholar]
  4. Barlow R. M., Middleton D. 1990; Dietary transmission of bovine spongiform encephalopathy to mice. Vet Rec 126:111–112
    [Google Scholar]
  5. Bons N., Mestre-Frances N., Belli P., Cathala F., Gajdusek C., Brown P. 1999; Natural and experimental oral infection of nonhuman primates by bovine spongiform encephalopathy agents. Proc Natl Acad Sci U S A 96:4046–4051
    [Google Scholar]
  6. Bruce M. E. 1985; Agent replication dynamics in a long incubation period model of mouse scrapie. J Gen Virol 66:2517–2522
    [Google Scholar]
  7. Bruce M. E., Chree A., McConnell I., Foster J., Pearson G., Fraser H. 1994; Transmission of bovine spongiform encephalopathy and scrapie to mice: strain variation and the species barrier. Phil Trans R Soc Lond Ser B Biol Sci 343:405–411
    [Google Scholar]
  8. Bruce M. E., Will R. G., Ironside J. W. 10 other authors 1997; Transmissions to mice indicate that ‘new variant’ CJD is caused by the BSE agent. Nature 389:498–501
    [Google Scholar]
  9. Collis S. C., Kimberlin R. H. 1985; Long term persistence of scrapie infection in mouse spleens in the absence of clinical disease. FEMS Microbiol Lett 29:111–114
    [Google Scholar]
  10. DAFS (Department of Agriculture & Fisheries) 1983 Results of June 1983 Annual Agricultural and Horticultural Census, Scotland Edinburgh: DAFS;
    [Google Scholar]
  11. Dawson M., Wells G. A. H., Parker B. N. J. 1990a; Preliminary evidence of the experimental transmissibility of bovine spongiform encephalopathy to cattle. Vet Rec 126:112–113
    [Google Scholar]
  12. Dawson M., Wells G. A. H., Parker B. N. J., Scott A. C. 1990b; Primary parenteral transmission of bovine spongiform encephalopathy to the pig. Vet Rec 127:338–339
    [Google Scholar]
  13. Dawson M., Wells G. A. H., Parker B. N. J., Scott A. C. 1991; Transmission studies of BSE in cattle, hamsters, pigs and domestic fowl. In Subacute Spongiform Encephalopathies pp  25–32 Edited by Bradley R., Savey M., Marchant B. Commission of the European Communities Dordrecht: Kluwer Academic Publishers;
    [Google Scholar]
  14. Dawson M., Wells G. A. H., Parker B. N. J., Francis M. E., Scott A. C., Hawkins S. A. C., Martin T. C., Simmons M. M., Austin A. R. 1994; Transmission studies of BSE in cattle, pigs and domestic fowl. In Transmissible Spongiform Encephalopathies pp  161–167 Edited by Bradley R., Marchant B. A Consultation on BSE with the Scientific Veterinary Committee of the Commission of the European Communities held in Brussels, 14–15 September 1993 Document VI/4131/94-EN Brussels: European Commission, Agriculture;
    [Google Scholar]
  15. Dickinson A. G., Meikle M. H., Fraser H. 1968; Identification of a gene which controls the incubation period of some strains of scrapie agent in mice. J Comp Pathol 78:293–299
    [Google Scholar]
  16. Dickinson A. G., Fraser H., Outram G. W. 1975; Scrapie incubation time can exceed natural lifespan. Nature 256:732–733
    [Google Scholar]
  17. Foster J. D., Hope J., Fraser H. 1993; Transmission of bovine spongiform encephalopathy in sheep and goats. Vet Rec 133:339–341
    [Google Scholar]
  18. Fraser H., Foster J. 1994; Transmission to mice, sheep and goats and bioassay of bovine tissues. In Transmissible Spongiform Encephalopathies pp  149–159 Edited by Bradley R., Marchant B. A Consultation on BSE with the Scientific Veterinary Committee of the Commission of the European Communities held in Brussels 14–15 September 1993. Document VI/4131/94-EN Brussels: European Commission, Agriculture;
    [Google Scholar]
  19. Fraser H., McConnell I., Wells G. A. H., Dawson M. 1988; Transmission of bovine spongiform encephalopathy to mice. Vet Rec 123:472
    [Google Scholar]
  20. Fraser H., Bruce M. E., Chree A., McConnell I., Wells G. A. H. 1992; Transmission of bovine spongiform encephalopathy and scrapie to mice. J Gen Virol 73:1891–1897
    [Google Scholar]
  21. Gibbs C. J. Jr, Gajdusek D. C., Amyx H. 1979; Strain variation in the viruses of Creutzfeldt–Jakob disease and kuru. In Slow Transmissible Disease of the Nervous System vol 2 pp  87–110 Edited by Prusiner S. B., Hadlow W. J. New York: Academic Press;
    [Google Scholar]
  22. Gorde J. M., Tamalet J., Toga M., Bert J. 1982; Changes in the nigrostriatal system following microinjection of an unconventional agent. Brain Res 240:87–93
    [Google Scholar]
  23. Hawkins S. A. C., Ryder S. J., Wells G. A. H., Austin A. R., Dawson M. 1998; Studies of experimental transmissibility of BSE and scrapie to pigs. In Proceedings of the 15th International Pig Veterinary Society Congress , Birmingham , July 5–9 1998
    [Google Scholar]
  24. Hill A. F., Desbrusliers M., Joiner S., Sidle K. C., Gowland I., Collinge J., Doey L. J., Lantos P. L. 1997; The same prion strain causes vCJD and BSE. Nature 389:448–451
    [Google Scholar]
  25. Hoinville L. J., Wilesmith J. W., Richards M. S. 1995; An investigation of risk factors for cases of bovine spongiform encephalopathy born after the introduction of the “feed ban”. Vet Rec 136:312–318
    [Google Scholar]
  26. Hunter N., Goldman W., Smith G., Hope J. 1994; Frequencies of PrP gene variants in healthy cattle and cattle with BSE in Scotland. Vet Rec 135:400–403
    [Google Scholar]
  27. Kim Y. S., Carp R. I., Callahan S. M., Wisniewski H. M. 1987; Incubation periods and survival times for mice injected stereotaxically with three scrapie strains in different brain regions. J Gen Virol 68:695–702
    [Google Scholar]
  28. Kimberlin R. H. 1990; Unconventional ‘slow’ viruses. In Topley and Wilson's Principles of Bacteriology . Virology and Immunity, 8th edn. pp  671–693 Edited by Collier L. H., Timbury M. C. London: Edward Arnold;
    [Google Scholar]
  29. Kimberlin R. H. 1993a; Modified pathogenesis of scrapie at first intracerebral passage from sheep to mice. In Abstracts of the IXth International Congress of Virology, P59-1, p. 318 Glasgow:
    [Google Scholar]
  30. Kimberlin R. H. 1993b; Bovine spongiform encephalopathy: an appraisal of the current epidemic in the United Kingdom. Intervirology 35:208–218
    [Google Scholar]
  31. Kimberlin R. H. 1994; A scientific evaluation of research into bovine spongiform encephalopathy (BSE). In Transmissible Spongiform Encephalopathies . pp  455–477 Edited by Bradley R., Marchant B. A Consultation on BSE with the Scientific Veterinary Committee of the Commission of the European Communities held in Brussels, 14–15 September 1993 Document VI/4131/94-EN Brussels: European Commission, Agriculture;
    [Google Scholar]
  32. Kimberlin R. H. 1996; Bovine spongiform encephalopathy (BSE) and public health: some problems and solutions in assessing the risks. In Transmissible Subacute Spongiform Encephalopathies: Prion Diseases pp  487–502 Edited by Court L., Dodet B. Paris: Elsevier;
    [Google Scholar]
  33. Kimberlin R. H., Wilesmith J. W. 1994 Bovine spongiform encephalopathy (BSE): epidemiology, low dose exposure and risks. In Slow Infections of the Central Nervous System: the Legacy of Dr . Bjorn Sigurdsson Edited by Bjornsson J., Carp R. I., Love A., Wisniewski H. M. Ann N Y Acad Sci 724, 210–220
    [Google Scholar]
  34. Kimberlin R. H., Field H. J., Walker C. A. 1983; Pathogenesis of mouse scrapie: evidence for spread of infection from central to peripheral nervous system. J Gen Virol 64:713–716
    [Google Scholar]
  35. Kimberlin R. H., Cole S., Walker C. A. 1987; Pathogenesis of scrapie is faster when infection is intraspinal instead of intracerebral. Microb Pathog 2:405–415
    [Google Scholar]
  36. Kimberlin R. H., Walker C. A., Fraser H. 1989; The genomic identity of different strains of mouse scrapie is expressed in hamsters and preserved on reisolation in mice. J Gen Virol 70:2017–2025
    [Google Scholar]
  37. Kirkwood J. K., Cunningham A. A. 1994; Spongiform encephalopathy in captive wild animals in Britain: epidemiological observations. In Transmissible Spongiform Encephalopathies pp  29–47 Edited by Bradley R., Marchant B. A Consultation on BSE with the Scientific Veterinary Committee of the Commission of the European Communities held in Brussels, 14–15 September 1993 Document VI/4131/94-EN Brussels: European Commission, Agriculture;
    [Google Scholar]
  38. Lasmézas C. I., Deslys J.-P., Demalmay R., Adjou K. T., Lamoury F., Dormont D., Robain O., Ironside J., Hauw J.-J. 1996; BSE transmission to macaques. Nature 381:743–744
    [Google Scholar]
  39. MAFF (Ministry of Agriculture, Fisheries & Food) 1983 Annual Agricultural and Horticultural Census, England and Wales London: MAFF;
    [Google Scholar]
  40. MAFF (Ministry of Agriculture, Fisheries & Food) 1995; Annual Agricultural and Horticultural Census . England and Wales York: MAFF;
    [Google Scholar]
  41. Martin T., Hughes S., Hughes K., Dawson M. 1995; Direct sequencing of PCR amplified pig PrP genes. Biochim Biophys Acta 1270:211–214
    [Google Scholar]
  42. Millson G. C., Kimberlin R. H., Manning E. J., Collis S. C. 1979; Early distribution of radioactive liposomes and scrapie infectivity in mouse tissues following administration by different routes. Vet Microbiol 4:89–99
    [Google Scholar]
  43. MLC (Meat & Livestock Commission 1999 MLC Pig Yearbook, 1999 p 59 Milton Keynes: MLC;
    [Google Scholar]
  44. MMC (Monopolies & Mergers Commission) 1985 Animal Waste: a Report on the Supply of Animal Waste in Great Britain , Appendix 2.1 London: HMSO;
    [Google Scholar]
  45. Race R., Chesebro B. 1998; Scrapie infectivity found in resistant species. Nature 392:770
    [Google Scholar]
  46. Robinson M. M., Hadlow W. J., Huff T. P., Wells G. A. H., Dawson M., Marsh R. F., Gorham J. R. 1994; Experimental infection of mink with bovine spongiform encephalopathy. J Gen Virol 75:2151–2155
    [Google Scholar]
  47. Ryder S. J., Hawkins S. A. C., Dawson M., Wells G. A. H. 2000; The neuropathology of experimental bovine spongiform encephalopathy in the pig. J Comp Pathol 122:131–143
    [Google Scholar]
  48. Simmons M. M., Harris P., Jeffrey M., Meek S. C., Blamire I. W. H., Wells G. A. H. 1996; BSE in Great Britain: consistency of the neurohistopathological findings in two random annual samples of clinically suspect cases. Vet Rec 138:175–177
    [Google Scholar]
  49. SOAEFD (Scottish Office, Agriculture, Environment & Fisheries Department) 1995 Results of June 1995 Annual Agricultural and Horticultural Census, Scotland Edinburgh: SOAEFD;
    [Google Scholar]
  50. Stack M. J., Keyes P., Scott A. C. 1996; The diagnosis of bovine spongiform encephalopathy and scrapie by the detection of fibrils and the abnormal protein isoform. In: Prion Diseases pp  85–103 Edited by Baker H., Ridley R. M. Totowa, NJ: Humana Press;
    [Google Scholar]
  51. Taylor D. M., Fernie K., Steele P. J., Somerville R. A. 2001; Relative efficiency of transmitting bovine spongiform encephalopathy to RIII mice by the oral route. Vet Rec 148:345–346
    [Google Scholar]
  52. Wells G. A. H., Scott A. C., Johnson C. T., Gunning R. F., Hancock R. D., Jeffrey M., Dawson M., Bradley R. 1987; A novel progressive spongiform encephalopathy in cattle. Vet Rec 121:419–420
    [Google Scholar]
  53. Wells G. A. H., Hawkins S. A. C., Hadlow W. J., Spencer Y. I. 1992; The discovery of bovine spongiform encephalopathy and observations on the vacuolar changes. In Prion Disease of Humans and Animals pp  256–274 Edited by Prusiner S. B., Collinge J., Powell J., Anderton B. Chichester: Ellis Horwood;
    [Google Scholar]
  54. Wells G. A. H., Dawson M., Hawkins S. A. C., Green R. B., Francis M. E., Simmons M. M., Austin A. R., Horigan M. W. 1994; Infectivity in the ileum of cattle challenged orally with bovine spongiform encephalopathy. Vet Rec 135:40–41
    [Google Scholar]
  55. Wells G. A. H., Dawson M., Hawkins S. A. C., Austin A. R., Green R. B., Dexter I., Horigan M. W., Simmons M. M. 1996; Preliminary observations on the pathogenesis of experimental bovine spongiforrn encephalopathy. In Bovine Spongiform Encephalopathy .The BSE Dilemma pp  28–44 Edited by Gibbs C. J. Jr New York: Springer-Verlag;
    [Google Scholar]
  56. Wells G. A. H., Hawkins S. A. C., Green R. B., Austin A. R., Dexter I., Spencer Y. I., Chaplin M. J., Stack M. J., Dawson M. 1998; Preliminary observations on the pathogenesis of experimental bovine spongiform encephalopathy (BSE): an update. Vet Rec 142:103–106
    [Google Scholar]
  57. Wells G. A. H., Hawkins S. A. C., Green R. B., Spencer Y. I., Dexter I., Dawson M. 1999; Limited detection of sternal bone marrow infectivity in the clinical phase of experimental bovine spongiform encephalopathy (BSE). Vet Rec 144:292–294
    [Google Scholar]
  58. Wilesmith J. W. 1991; The epidemiology of bovine spongiform encephalopathy. Semin Virol 2:239–245
    [Google Scholar]
  59. Wilesmith J. W. 1998; Manual on Bovine Spongiform Encephalopathy . p–51 FAO Animal Health Manual No. 2. Rome: Food and Agriculture Organization of the United Nations
  60. Wilesmith J. W., Wells G. A. H., Cranwell M. P., Ryan J. B. M. 1988; Bovine spongiform encephalopathy: epidemiological studies. Vet Rec 123:638–644
    [Google Scholar]
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