%0 Journal Article %A Zanusso, Gianluigi %A Casalone, Cristina %A Acutis, Pierluigi %A Bozzetta, Elena %A Farinazzo, Alessia %A Gelati, Matteo %A Fiorini, Michele %A Forloni, Gianluigi %A Sy, Man Sun %A Monaco, Salvatore %A Caramelli, Maria %T Molecular analysis of iatrogenic scrapie in Italy %D 2003 %J Journal of General Virology, %V 84 %N 4 %P 1047-1052 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.18774-0 %I Microbiology Society, %X An accidental intra- and interspecies transmission of scrapie occurred in Italy in 1997 and 1998 following exposure to a vaccine against Mycoplasma agalactiae. PrPSc in affected sheep and goats, collected from a single flock exposed to vaccination 2 years earlier, was molecularly typed. In five animals with iatrogenic scrapie, a PrPSc type with a 20 kDa core fragment was found in all areas of the brain investigated. In three sheep and one goat, this isoform co-occurred with a fully glycosylated isoform that had a protease-resistant backbone of 17 kDa, whereas in two sheep and four goats, the two PrPSc types were detected in different regions of the brain. In sheep with natural field scrapie, a PrPSc type with physico-chemical properties indistinguishable from the 20 kDa isoform was found. The present results suggest the co-presence of two prion strains in mammary gland and brain homogenates used for vaccination. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.18774-0