@article{mbs:/content/journal/jgv/10.1099/vir.0.18773-0, author = "Nagai, Makoto and Sakoda, Yoshihiro and Mori, Masashi and Hayashi, Michiko and Kida, Hiroshi and Akashi, Hiroomi", title = "Insertion of cellular sequence and RNA recombination in the structural protein coding region of cytopathogenic bovine viral diarrhoea virus", journal= "Journal of General Virology", year = "2003", volume = "84", number = "2", pages = "447-452", doi = "https://doi.org/10.1099/vir.0.18773-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.18773-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The cytopathogenic bovine viral diarrhoea virus (cp BVDV) strain KS86-1cp was isolated from a calf persistently infected with the noncytopathogenic (ncp) strain KS86-1ncp after it was exposed to cp BVDV strain Nose and developed mucosal disease (MD). Molecular analysis revealed that an insertion of a cellular gene and a duplication of the viral RNA encoding the nucleocapsid protein C and part of Npro had occurred in the C coding region of the Nose and KS86-1cp genomes. The inserted cellular gene was closely related to the cINS sequence. Remarkably, the 5′ upstream region from the insertion of KS86-1cp had high sequence identity to that of Nose, but differed from that of KS86-1ncp. In contrast, the region downstream from the insertion of KS86-1cp showed high identity to KS86-1ncp, but not to Nose. These data reveal that KS86-1cp is a chimeric virus generated by homologous RNA recombination in a calf with MD.", }