1887

Abstract

Efficient gene delivery of a baculovirus-derived vector (BV-p53-lacZ) to a human osteogenic sarcoma cell line, Saos-2, was serendipitously found while evaluating the vector for gene delivery to human p53-null tumour cells in a previous study. Therefore, we investigated other human, rat and mouse osteogenic sarcoma and other types of tumour cell lines for transduction efficiency via baculovirus vectors containing a reporter gene under the control of either a cytomegalovirus or Rous sarcoma virus promoter. The expression of β-galactosidase protein, assessed by X-Gal staining and β-galactosidase ELISA, demonstrated an extremely high level of transduction efficiency in some osteogenic sarcoma cell lines, such as U-2OS, Saos-2 and Saos-LM2. These human osteogenic sarcoma cell lines showed levels of β-galactosidase expression 5–40 times greater than HepG2 cells, which were previously thought to be the mammalian cells most susceptible to baculovirus-mediated gene delivery. The level of acetylated histone proteins in these tumour lines did not correlate well with the high level of reporter gene expression. These results strongly suggest that some osteogenic sarcoma cells are highly susceptible to baculovirus-mediated gene delivery and that a baculovirus-derived vector is an efficient gene delivery vehicle into human osteogenic sarcoma cells.

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2003-03-01
2020-09-23
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References

  1. Bishop D. H. L., Entwistle P. F., Cameron I. R., Allen C. J., Possee R. D.. 1988; In Field Trials of Genetically Engineered Baculovirus Insecticides . pp 143–179 Edited by Sussman M., Collins C. H., Skinner F. A., Stewart-Tull D. E.. London: Academic Press;
  2. Boyce F. M., Bucher N. L. R.. 1996; Baculovirus-mediated gene transfer into mammalian cells. Proc Natl Acad Sci U S A93:2348–2352
    [Google Scholar]
  3. Carbonell L. F., Klowen M. J., Miller L. K.. 1985; Baculovirus-mediated expression of bacterial genes in dipteran and mammalian cells. J Virol56:153–160
    [Google Scholar]
  4. Charlton C. A., Volkman L. E.. 1993; Penetration of Autographa californica nuclear polyhedrosis virus nucleocapsids into IPLB Sf 21 cells induces actin cable formation. Virology197:245–254
    [Google Scholar]
  5. Condreay J. P., Witherspoon S. M., Clay W. C., Kost T. A.. 1999; Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector. Proc Natl Acad Sci U S A96:127–132
    [Google Scholar]
  6. Cory J. S., Bishop D. H.. 1997; Use of baculoviruses as biological insecticides. Mol Biotechnol 7303–313
    [Google Scholar]
  7. Duisit G., Saleun S., Douthe S., Barsoum J., Chadeuf G., Moullier P.. 1999; Baculovirus vector requires electrostatic interactions including heparan sulfate for efficient gene transfer in mammalian cells. J Gene Med1:93–102
    [Google Scholar]
  8. Hofmann C., Strauss M.. 1998; Baculovirus-mediated gene transfer in the presence of human serum or blood facilitated by inhibition of the complement system. Gene Ther5:531–536
    [Google Scholar]
  9. Hofmann C., Sandig V., Jennings G., Rudolph M., Schlag P., Strauss M.. 1995; Efficient gene transfer into human hepatocytes by baculovirus vectors. Proc Natl Acad Sci U S A92:10099–10103
    [Google Scholar]
  10. Luckow V. A., Summers M. D.. 1988; Trends in the development of baculovirus expression vectors. Biotechnology6:47–55
    [Google Scholar]
  11. Matsuura Y., Possee R. D., Overton H. A., Bishop D. H. L.. 1987; Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. J Gen Virol68:1233–1250
    [Google Scholar]
  12. Mulligan R. C.. 1993; The basic science of gene therapy. Science260:926–932
    [Google Scholar]
  13. Pennock G. D., Shoemaker C., Miller L. K.. 1984; Strong and regulated expression of Escherichia coli beta-galactosidase in insect cells with a baculovirus vector. Mol Cell Biol4:399–406
    [Google Scholar]
  14. Sarkis C., Serguera C., Petres S., Buchet D., Ridet J.-L., Edelman L., Mallet J.. 2000; Efficient transduction of neural cells in vitro and in vivo by a baculovirus-derived vector. Proc Natl Acad Sci U S A97:14638–14643
    [Google Scholar]
  15. Shoji I., Aizaki H., Tani H., Ishii K., Chiba T., Saito I., Miyamura T., Matsuura Y.. 1997; Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors. J Gen Virol78:2657–2664
    [Google Scholar]
  16. Smith G. E., Summers M. D., Fraser M. J.. 1983; Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol Cell Biol3:2156–2165
    [Google Scholar]
  17. Song S. U., Boyce F. M.. 2001; Combination treatment for osteosarcoma with baculoviral vector mediated gene therapy (p53) and chemotherapy (adriamycin). Exp Mol Med33:46–53
    [Google Scholar]
  18. Tani H., Nishijima M., Ushijima H., Miyamura T., Matsuura Y.. 2001; Characterization of cell-surface determinants important for baculovirus infection. Virology279:343–353
    [Google Scholar]
  19. van Loo N. D., Fortunati E., Ehlert E., Rabelink M. Grosveld F., Scholte B. J.. 2001; Baculovirus infection of nondividing mammalian cells: mechanisms of entry and nuclear transport of capsids. J Virol75:961–970
    [Google Scholar]
  20. Verma I. M., Somia N.. 1997; Gene therapy – promises, problems, and prospects. Nature389:239–242
    [Google Scholar]
  21. Volkman L. E., Goldsmith P. A.. 1985; Mechanism of neutralization of budded Autographa californica nuclear polyhedrosis virus by a monoclonal antibody: inhibition of entry by adsorptive endocytosis. Virol143:185–195
    [Google Scholar]
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