1887

Abstract

A Sabin 3/Sabin 2/Sabin 3 (S3/2/3) intertypic recombinant poliovirus was isolated from a faecal specimen from a 2-year-old healthy boy approximately 12 weeks after administration of oral poliovirus vaccine. The first recombination junction was in the genomic region encoding the VP1 capsid protein between nucleotide positions 3274 and 3285 (numbering according to Sabin 3) and the second was in the RNA polymerase region (nucleotide positions 6824 and 6825). The recombination had introduced six Sabin 2-derived amino acids into the Sabin 3 capsid environment in the carboxyl terminus of VP1. The complete genome of the recombinant virus differed from corresponding parental Sabin strains at 33 nucleotide positions, nine of them resulting in an amino acid substitution. Four substitutions were in the capsid proteins and five were in the region encoding the non-structural proteins. One amino acid was changed in the antigenic site 2B and two in site 3B. In addition, the whole antigenic site 3A was replaced by Sabin 2-specific amino acids, but the antigenic characteristics of the S3/2/3 did not show type 2-specific features. Neutralizing antibody titres in sera from Finnish children immunized with the inactivated poliovirus vaccine were not lower against the recombinant virus than against Sabin 3. Our results suggest that the chimeric virus was most likely generated by recombination events in the vaccinee, rather than representing progeny of circulating vaccine-derived virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.18708-0
2003-03-01
2020-08-12
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/3/vir840573.html?itemId=/content/journal/jgv/10.1099/vir.0.18708-0&mimeType=html&fmt=ahah

References

  1. Abraham G., Colonno R. J.. 1984; Many rhinovirus serotypes share the same cellular receptor. J Virol51:340–345
    [Google Scholar]
  2. Balanant J., Guillot S., Candrea A., Delpeyroux F., Crainic R.. 1991; The natural genomic variability of poliovirus analysed by a restriction fragment length polymorphism assay. Virology184:645–654
    [Google Scholar]
  3. Blomqvist S., Skyttä A., Roivainen M., Hovi T.. 1999; Rapid detection of human rhinoviruses in nasopharyngeal aspirates by a microwell reverse transcription-PCR-hybridisation assay. J Clin Microbiol37:2813–2816
    [Google Scholar]
  4. Caro V., Guillot S., Delpeyroux F., Crainic R.. 2001; Molecular strategy for ‘serotyping’ of human enteroviruses. J Gen Virol82:79–91
    [Google Scholar]
  5. Centers for Disease Control & Prevention. 2001a; Circulation of a type 2 vaccine-derived poliovirus – Egypt, 1982–1993. J Am Med Assoc285:1148–1149
    [Google Scholar]
  6. Centers for Disease Control & Prevention. 2001b; Acute flaccid paralysis associated with circulating vaccine-derived poliovirus –. Philippines: 2001; Morb Mortal Wkly Rep50:874–875
    [Google Scholar]
  7. Chumakov K. M., Norwood L. P., Parker M. L., Dragunsky E. M., Ran Y., Levenbook I. S.. 1992; RNA sequence variants in live poliovirus vaccine and their relation to neurovirulence. J Virol66:966–970
    [Google Scholar]
  8. Cuervo N. S., Guillot S., Romanenkova N., Combiescu M., Aubert-Combiescu A., Seghier M., Caro V., Crainic R., Delpeyroux F.. 2001; Genomic features of intertypic recombinant Sabin poliovirus strains excreted by primary vaccinees. J Virol75:5740–5751
    [Google Scholar]
  9. Evans D. M. A., Dunn G., Minor P. D., Schild G. C., Cann A. J., Stanway G., Almond J. W., Currey K., Maizel J. V.. 1985; A single nucleotide change in the 5′ non-coding region of the genome of the Sabin type 3 polio vaccine is associated with increased neurovirulence. Nature314:548–550
    [Google Scholar]
  10. Furione M., Guillot S., Otelea D., Balanant J., Candrea A., Crainic R.. 1993; Polioviruses with natural recombinant genomes isolated from vaccine-associated paralytic poliomyelitis. Virology196:199–208
    [Google Scholar]
  11. Cammack N. J., Phillips A., Dunn G., Patel V., Minor P. D.. 1989; Intertypic genomic rearrangements of poliovirus strains in vaccinees. Virology167:507–514
    [Google Scholar]
  12. Georgescu M. M., Delpeyroux F., Tardy-Panit M., Balanant J., Combiescu M., Combiescu A. A., Guillot S., Crainic R.. 1994; High diversity of polio vaccine strains isolated from the central nervous system of patients with vaccine-associated paralytic poliomyelitis. J Virol68:8089–8101
    [Google Scholar]
  13. Georgescu M. M., Delpeyroux F., Crainic R.. 1995; Tripartite genome organization of a natural type 2 vaccine/nonvaccine recombinant poliovirus. J Gen Virol76:2343–2348
    [Google Scholar]
  14. Georgopoulou A., Markoulatos P.. 2001; Sabin type 2 polioviruses with intertypic vaccine/vaccine recombinant genomes. Eur J Clin Microbiol Infect Dis20:792–799
    [Google Scholar]
  15. Guillot S., Caro V., Cuervo N., Korotkova E., Combiescu M., Persu A., Aubert-Combiescu A., Delpeyroux F., Crainic R.. 2000; Natural genetic exchanges between vaccine and wild poliovirus strains in humans. J Virol74:8434–8443
    [Google Scholar]
  16. Huovilainen A., Kinnunen L., Ferguson M., Hovi T.. 1988; Antigenic variation among 173 strains of type 3 poliovirus isolated in Finland during the 1984 to 1985 outbreak. J Gen Virol69:1941–1948
    [Google Scholar]
  17. Jarvis T. C., Kirkegaard K.. 1992; Poliovirus RNA recombination – mechanistic studies in the absence of selection. EMBO J11:3135–3145
    [Google Scholar]
  18. Kew O., Morris-Glasgow V., Landaverde M.. 21 other authors 2002; Outbreak of poliomyelitis in Hispaniola associated with circulating type 1 vaccine-derived poliovirus. Science296:356–359
    [Google Scholar]
  19. Kilpatrick D. R., Nottay B., Yang C., Yang S., Mulders M. N., Holloway B., Pallansch M. A., Kew O. M.. 1996; Group-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residues at positions of codon degeneracy. J Clin Microbiol34:2990–2996
    [Google Scholar]
  20. Kinnunen L., Pöyry T., Hovi T.. 1991; Generation of virus genetic lineages during an outbreak of poliomyelitis. J Gen Virol72:2483–2489
    [Google Scholar]
  21. Lipskaya G. Y., Muzychenko A. R., Kutitova O. K., Maslova S. V., Equestre M., Drozdov S. G., Bercoff R. P., Agol V. I.. 1991; Frequent isolation of intertypic poliovirus recombinants with serotype 2 specificity from vaccine-associated polio cases. J Med Virol35:290–296
    [Google Scholar]
  22. Liu H. M., Zheng D. P., Zhang L. B., Oberste M. S., Pallansch M. A., Kew O. M.. 2000; Molecular evolution of a type 1 wild-vaccine poliovirus recombinant during widespread circulation in China. J Virol74:11153–11161
    [Google Scholar]
  23. Macadam A. J., Arnold C., Howlett J.. 9 other authors 1989; Reversion of the attenuated and temperature-sensitive phenotypes of the sabin type 3 strain of poliovirus in vaccinees. Virology172:408–414
    [Google Scholar]
  24. Martin J., Samoilovich E., Dunn G., Lackenby A., Feldman E., Heath A., Svirchevskaya E., Cooper G., Yermalovich M., Minor P. D.. 2002; Isolation of an intertypic poliovirus capsid recombinant from a child with vaccine-associated paralytic poliomyelitis. J Virol76:10921–10928
    [Google Scholar]
  25. Minor P. D., Dunn G.. 1988; The effect of sequences in the 5′ non-coding region on the replication of polioviruses in the human gut. J Gen Virol69:1091–1096
    [Google Scholar]
  26. Minor P. D., Ferguson M., Evans D. M. A., Almond J. W., Icenogle J. P.. 1986; Antigenic structure of polioviruses of serotypes 1, 2 and 3. J Gen Virol67:1283–1291
    [Google Scholar]
  27. Minor P. D., Dunn G., Evans D. M. A.. 8 other authors 1989; The temperature sensitivity of the Sabin type 3 vaccine strain of poliovirus: molecular and structural effects of a mutation in the capsid protein VP3. J Gen Virol70:1117–1123
    [Google Scholar]
  28. Mulders M. N., Lipskaya G. Y., van der Avoort H. G., Koopmans M. P., Kew O. M., van Loon A. M.. 1995; Molecular epidemiology of wild poliovirus type 1 in Europe, the Middle East, and the Indian subcontinent. J Infect Dis171:1399–1405
    [Google Scholar]
  29. Mulders M. N., Reimerink J. H. J., Stenvik M., Alaeddinoglu I., van der Avoort H. G. A. M., Hovi T., Koopmans M. P. G.. 1999; A Sabin vaccine-derived field isolate of poliovirus type 1 displaying aberrant phenotypic and genetic features, including a deletion in antigenic site 1. J Gen Virol80:907–916
    [Google Scholar]
  30. Reimerink J. H. J., van der Avoort H. G. A. M., van Loon A. M., Koopmans M. P. G.. 1999; Genetic basis for immunological aberrations in poliovirus Sabin serotype 3 strains imported in The Netherlands. J Clin Microbiol37:2393–2398
    [Google Scholar]
  31. Rico-Hesse R., Pallansch M. A., Nottay B. K., Kew O. M.. 1987; Geographic distribution of wild type poliovirus type 1 genotypes. Virology160:311–322
    [Google Scholar]
  32. Sabin A. B., Boulger L. R.. 1973; History of Sabin attenuated poliovirus oral live vaccine strains. J Biol Stand1:115–118
    [Google Scholar]
  33. Stanway G., Cann A. J., Hauptmann R., Hughes P., Clarke L. D., Mountford R. C., Minor P. D., Schildt G. C., Almond J. W.. 1983; The nucleotide sequence of poliovirus type 3 Leon 12 a1b: comparison with poliovirus type 1. Nucleic Acids Res11:5629–5643
    [Google Scholar]
  34. WHO 2001; Polio Laboratory Manual Geneva: World Health Organization;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.18708-0
Loading
/content/journal/jgv/10.1099/vir.0.18708-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error