1887

Abstract

The genome of bovine herpesvirus type 1 Schönböken was cloned as a bacterial artificial chromosome (BAC) by inserting mini F plasmid sequences into the glycoprotein (g) E gene. The resulting BAC clone, pBHV-1ΔgE, was transfected into bovine kidney cells and viable gE-negative BHV-1 (BHV-1ΔgE) was recovered. By RecE/T mutagenesis in , the gG open reading frame was deleted from pBHV-1ΔgE. From the mutated BAC, double negative BHV-1ΔgE-gG was reconstituted and its growth properties were compared to those of rescuant viruses in which the gE gene was restored (BHV-1rev, BHV-1ΔgG). The mutant viruses did not exhibit markedly lowered virus titres. Plaque sizes of BHV-1ΔgE, BHV-1ΔgE-gG and BHV-1ΔgG, however, were reduced by 19 to 55 % compared to parental strain Schönböken or BHV-1rev. Our results suggested that gE and gG function independently from each other in cell-to-cell spread, because an additive effect on plaque formation was observed in the gE/gG double deletion mutant.

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2003-02-01
2020-01-23
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References

  1. Adler, H., Messerle, M., Wagner, M. & Koszinowski, U. H. ( 2000; ). Cloning and mutagenesis of the murine gammaherpesvirus 68 genome as an infectious bacterial artificial chromosome. J Virol 74, 6964–6974.[CrossRef]
    [Google Scholar]
  2. Belknap, E. B., Walters, L. M., Kelling, C., Ayers, V. K., Norris, J., McMillen, J., Hayhow, C., Cochran, M., Reddy, D. N., Wright, J. & Collins, J. K. ( 1999; ). Immunogenicity and protective efficacy of a gE, gG and US2 gene-deleted bovine herpesvirus-1 (BHV-1) vaccine. Vaccine 17, 2297–2305.[CrossRef]
    [Google Scholar]
  3. Datsenko, K. A. & Wanner, B. L. ( 2000; ). One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97, 6640–6645.[CrossRef]
    [Google Scholar]
  4. Engelhardt, T. & Keil, G. M. ( 1996; ). Identification and characterization of the bovine herpesvirus 5 US4 gene and gene products. Virology 225, 126–135.[CrossRef]
    [Google Scholar]
  5. Fehler, F., Herrmann, J. M., Saalmüller, A., Mettenleiter, T. C. & Keil, G. M. ( 1992; ). Glycoprotein IV of bovine herpesvirus 1-expressing cell line complements and rescues a conditionally lethal viral mutant. J Virol 66, 831–839.
    [Google Scholar]
  6. Gibbs, E. P. J. & Rweyemamu, M. M. ( 1977; ). Bovine herpesviruses. Part I. Bovine herpesvirus 1. Vet Bull 47, 317–343.
    [Google Scholar]
  7. Highlander, S. L., Sutherland, S. L., Gage, P. J., Johnson, D. C., Levine, M. & Glorioso, J. C. ( 1987; ). Neutralizing monoclonal antibodies specific for herpes simplex virus glycoprotein D inhibit virus penetration. J Virol 61, 3356–3364.
    [Google Scholar]
  8. Keil, G. M., Engelhardt, T., Karger, A. & Enz, M. ( 1996; ). Bovine herpesvirus 1 Us open reading frame 4 encodes a glycoproteoglycan. J Virol 70, 3032–3038.
    [Google Scholar]
  9. McGeoch, D. J. ( 1990; ). Evolutionary relationships of virion glycoprotein genes in the S regions of alphaherpesvirus genomes. J Gen Virol 71, 2361–2367.[CrossRef]
    [Google Scholar]
  10. Mahony, T. J., McCarthy, F. M., Gravel, J. L., West, L. & Young, P. L. ( 2002; ). Construction and manipulation of an infectious clone of the bovine herpesvirus 1 genome maintained as a bacterial artificial chromosome. J Virol 76, 6660–6668.[CrossRef]
    [Google Scholar]
  11. Matheka, H. D. & Straub, O. C. ( 1972; ). Weitere Untersuchungen zur Differenzierung des Virus der Rhinotracheitis (IBR) vom Virus des Bläschenausschlages (IPV) durch die trägerfreie Zonenelektrophorese in einem Glukose-Dichtegradienten. Archiv Gesamte Virusforsch 39, 265–273.[CrossRef]
    [Google Scholar]
  12. Nakamichi, K., Ohara, K., Kuroki, D. & Otsuka, H. ( 2000; ). Bovine herpesvirus 1 glycoprotein G is required for viral growth by cell-to-cell infection. Virus Res 68, 175–181.[CrossRef]
    [Google Scholar]
  13. Nakamichi, K., Matsumoto, Y. & Otsuka, H. ( 2002; ). Bovine herpesvirus 1 glycoprotein G is necessary for maintaining cell-to-cell junctional adherence among infected cells. Virology 294, 22–30.[CrossRef]
    [Google Scholar]
  14. Neubauer, A., Beer, M., Brandmüller, C., Kaaden, O. R. & Osterrieder, N. ( 1997; ). Equine herpesvirus 1 mutants devoid of glycoprotein B or M are apathogenic for mice but induce protection against challenge infection. Virology 239, 36–45.[CrossRef]
    [Google Scholar]
  15. Rebordosa, X., Pinol, J., Perez-Pons, J. A., Lloberas, J., Naval, J., Serra-Hartmann, X., Espuna, E. & Querol, E. ( 1996; ). Glycoprotein E of bovine herpesvirus type 1 is involved in virus transmission by direct cell-to-cell spread. Virus Res 45, 59–68.[CrossRef]
    [Google Scholar]
  16. Rudolph, J. & Osterrieder, N. ( 2002; ). Equine herpesvirus type 1 devoid of gM and gp2 is severely impaired in virus egress but not direct cell-to-cell spread. Virology 293, 356–367.[CrossRef]
    [Google Scholar]
  17. Rudolph, J., Seyboldt, C., Granzow, H. & Osterrieder, N. ( 2002; ). The gene 10 (UL49.5) product of equine herpesvirus 1 is necessary and sufficient for functional processing of glycoprotein M. J Virol 76, 2952–2963.[CrossRef]
    [Google Scholar]
  18. Schröder, C., Linde, G., Fehler, F. & Keil, G. M. ( 1997; ). From essential to beneficial: glycoprotein D loses importance for replication of bovine herpesvirus 1 in cell culture. J Virol 71, 25–33.
    [Google Scholar]
  19. Schumacher, D., Tischer, B. K., Fuchs, W. & Osterrieder, N. ( 2000; ). Reconstitution of Marek's disease virus serotype 1 (MDV-1) from DNA cloned as a bacterial artificial chromosome and characterization of a glycoprotein B-negative MDV-1 mutant. J Virol 74, 11088–11098.[CrossRef]
    [Google Scholar]
  20. Schwyzer, M. & Ackermann, M. ( 1996; ). Molecular virology of ruminant herpesviruses. Vet Microbiol 53, 17–29.[CrossRef]
    [Google Scholar]
  21. Tischer, B. K., Schumacher, D., Messerle, M., Wagner, M. & Osterrieder, N. ( 2002; ). The products of the UL10 (gM) and the UL49.5 genes of Marek's disease virus serotype 1 are essential for virus growth in cultured cells. J Gen Virol 83, 997–1003.
    [Google Scholar]
  22. van Regenmortel, M. H. V., Fauquet, C. M., Bishop, D. H. L., Carstens, E. B., Estes, M. K., Lemon, S. M., Maniloff, M. A., Mayo, D. J., McGeoch, D. J., Pringle, C. R. & Wickner, R. B. ( 2000; ). Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses. San Diego: Academic Press.
  23. Whitbeck, J. C., Knapp, A. C., Enquist, L. W., Lawrence, W. C. & Bello, L. J. ( 1996; ). Synthesis, processing, and oligomerization of bovine herpesvirus 1 gE and gI membrane proteins. J Virol 70, 7878–7884.
    [Google Scholar]
  24. Yoshitake, N., Xuan, X. & Otsuka, H. ( 1997; ). Identification and characterization of bovine herpesvirus-1 glycoproteins E and I. J Gen Virol 78, 1399–1403.
    [Google Scholar]
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