1887

Abstract

Genetic diversity and biological variation were compared for California isolates of (CMV). These fell into five pathotypes based on their reactions on three cucurbits including a susceptible squash, a melon with conventional resistance and a commercial CMV-resistant transgenic squash. Thirty-three isolates infected and caused symptoms on CMV-resistant transgenic squash. Forty-two isolates infected the CMV-resistant melon, but only 25 isolates infected both. Single-strand conformation polymorphism (SSCP) analysis was used to differentiate 81 California isolates into 14 groups, and the coat protein (CP) genes of 27 isolates with distinct and indistinguishable SSCP patterns were sequenced. Fourteen isolates corresponding to the different SSCP patterns were also used for phylogenetic analysis. Seventy-nine isolates belonged to CMV subgroup IA, but two belonged to CMV subgroup IB. This is the first report of subgroup IB isolates in the Americas. All CMV isolates had a nucleotide identity greater than or equal to 93·24 %. There was no correlation between CP gene variation and geographical origin, collection year, original host plant, or between the degree of CP amino acid sequence identity and the capacity to overcome transgenic and/or conventional resistance. SSCP and sequence analyses were used to compare 33 CMV isolates on CMV-resistant transgenic squash and susceptible pumpkin plants. One isolate showed sequence differences between these two hosts, but this was not due to recombination or selection pressure of transgenic resistance. CMV isolates capable of infecting cucurbits with conventional and transgenic CMV resistance were present in California, even before CMV transgenic material was available.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.18673-0
2003-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/1/vir840249.html?itemId=/content/journal/jgv/10.1099/vir.0.18673-0&mimeType=html&fmt=ahah

References

  1. Aaziz R., Tepfer M. 1999; Recombination in RNA viruses and in virus-resistant transgenic plants. J Gen Virol 80:1339–1346
    [Google Scholar]
  2. Allison R. F., Schneider W. L., Greene A. E. 1996; Recombination in plants expressing viral transgenes. Semin Virol 7:417–422
    [Google Scholar]
  3. Anderson E. J., Stark D. M., Nelson R. S., Powell P. A., Tumer N. E., Beachy R. N. 1989; Transgenic plants that express the coat protein genes of tobacco mosaic virus of alfalfa mosaic virus interfere with disease development of some nonrelated viruses. Phytopathology 79:1284–1290
    [Google Scholar]
  4. Beachy R. N. 1997; Mechanisms and applications of pathogen-derived resistance in transgenic plants. Curr Opin Biotechnol 8:215–220
    [Google Scholar]
  5. Daniels J., Campbell R. N. 1992; Characterization of cucumber mosaic virus isolates from California. Plant Dis 76:1245–1250
    [Google Scholar]
  6. Domingo E., Holland J., Biebricher C., Eigen M. 1995; Quasi-species: the concept and the word. In Molecular Basis of Virus Evolution pp  181–191 Edited by Gibbs A. J., Calisher C. H. K., García-Arenal F. Cambridge: Cambridge University Press;
    [Google Scholar]
  7. Eigen M. 1996; On the nature of virus quasispecies. Trends Microbiol 4:216–218
    [Google Scholar]
  8. Enzie W. D. 1943; A source of muskmelon mosaic resistance found in the oriental pickling melon, Cucumis melo var. Conomon . Proc Am Soc Hortic Sci 43:195–198
    [Google Scholar]
  9. Felsenstein J. 1989; phylip – Phylogenetic Inference Package (Version 3.2). Cladistics 5:164–166
    [Google Scholar]
  10. Ferreira S. A., Pitz K. Y., Manshardt R., Zee F., Fitch M., Gonsalves D. 2002; Virus coat protein transgenic papaya provides practical control of Papaya ringspot virus in Hawaii. Plant Dis 86:101–105
    [Google Scholar]
  11. Fraile A., Alonso-Prados J. L., Aranda M. A., Bernal J. J., Malpica J. M., Garcia-Arenal F. 1997; Genetic exchange by recombination or reassortment is infrequent in natural populations of a tripartite RNA plant virus. J Virol 71:934–940
    [Google Scholar]
  12. Gal S., Pisan B., Hohn T., Grimsley N., Hohn B. 1992; Agroinfection of a transgenic plant leads to viable cauliflower mosaic virus by intermolecular recombination. Virology 187:525–533
    [Google Scholar]
  13. Garcia-Arenal F., Fraile A., Malpica J. M. 2001; Variability and genetic structure of plant virus populations. Annu Rev Phytopathol 39:157–186
    [Google Scholar]
  14. Gonsalves D. 1998; Control of papaya ringspot virus in papaya: a case study. Annu Rev Phytopathol 36:415–437
    [Google Scholar]
  15. Greene A. E., Allison R. F. 1994; Recombination between viral RNA and transgenic plant transcripts. Science 263:1423–1425
    [Google Scholar]
  16. Greene A. E., Allison R. F. 1996; Deletions in the 3′ untranslated region of cowpea chlorotic mottle virus transgene reduce recovery of recombinant viruses in transgenic plants. Virology 225:231–234
    [Google Scholar]
  17. Holland J. J., De La Torre J. C., Steinhauer D. A. 1992; RNA virus population as quasispecies. Curr Top Microbiol Immunol 176:1–20
    [Google Scholar]
  18. Nelson R. S., Powell P. A., Beachy R. N. 1987; Lesions and virus accumulation in inoculated transgenic tobacco plants expressing the coat protein gene of tobacco mosaic virus. Virology 158:126–132
    [Google Scholar]
  19. Nelson R. S., McCormick S. M., Dellanay X. 9 other authors 1988; Virus tolerance, plant growth, and field performance of transgenic tomato plants expressing coat protein from tobacco mosaic virus. Bio/Technology 6:403–409
    [Google Scholar]
  20. Palukaitis P., Roossinck M. J., Dietzgen R. G., Francki R. I. B. 1992; Cucumber mosaic virus. Adv Virus Res 41:281–348
    [Google Scholar]
  21. Provvidenti R. 1993; Resistance to viral diseases of cucurbits. In Resistance to Viral Diseases of Vegetables: Genetics and Breeding pp  8–43 Edited by Kyle M. M. Portland, OR: Timber Press;
    [Google Scholar]
  22. Quemada H. D., Tricoli D. M. 1995; Petition for determination of regulatory status of squash line CZW-3 containing the coat protein genes from cucumber mosaic virus (CMV), watermelon mosaic virus 2 (WMV 2), and zucchini yellow mosaic virus (ZYMV). USA: USDA petition number 95-352-01p
    [Google Scholar]
  23. Quemada H. D., Gonsalves D., Slightom J. L. 1991; Expression of coat protein gene from cucumber mosaic virus strain C in tobacco: protection against infection by CMV strains transmitted mechanically or by aphids. Phytopathology 81:794–802
    [Google Scholar]
  24. Robinson D. J. 1996; Environmental risk assessment of release of transgenic plants containing virus-derived inserts. Transgenic Res 5:359–362
    [Google Scholar]
  25. Rodriguez-Alvarado G., Kurath G., Dodds J. A. 1995; Heterogeneity in pepper isolates of cucumber mosaic virus. Plant Dis 79:450–455
    [Google Scholar]
  26. Roossinck M. J. 2002; Evolutionary history of cucumber mosaic virus deduced by phylogenetic analyses. J Virol 76:3382–3387
    [Google Scholar]
  27. Roossinck M. J., Zhang L., Hellwald K.-H. 1999; Rearrangements in the 5′ nontranslated region and phylogenetic analyses of cucumber mosaic virus RNA 3 indicate radial evolution of three subgroups. J Virol 73:6752–6758
    [Google Scholar]
  28. Rubio L., Soong J., Kao J., Falk B. W. 1999; Geographic distribution and molecular variation of isolates of three whitefly-borne closteroviruses of cucurbits: lettuce infectious yellows virus, cucurbit yellow stunting disorder virus, and beet pseudo-yellows virus. Phytopathology 89:707–711
    [Google Scholar]
  29. Rubio L., Abou-Jawdah Y., Lin H. X., Falk B. W. 2001; Geographically distinct isolates of the crinivirus cucurbit yellow stunting disorder virus show very low genetic diversity in the coat protein. J Gen Virol 82:929–933
    [Google Scholar]
  30. Sasaya T., Yamamoto T. 1995; Improvements in non-precoated indirect enzyme-linked immunosorbent assay for specific detection of three potyviruses infecting cucurbitaceous plants. Ann Phytopathol Soc Jpn 61:130–133
    [Google Scholar]
  31. Schoelz J. E., Wintermantel W. M. 1993; Expansion of viral host range through complementation and recombination in transgenic plants. Plant Cell 5:1669–1679
    [Google Scholar]
  32. Tepfer M. 1993; Viral genes and transgenic plants. What are the potential environmental risks?. Bio/Technology 11:1125–1132
    [Google Scholar]
  33. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  34. Tricoli D. M., Carney K. J., Russell P. F. 7 other authors 1995; Field evaluation of transgenic squash containing single or multiple virus coat protein gene constructs for resistance to cucumber mosaic virus, watermelon mosaic virus 2, and zucchini yellow mosaic virus. Bio/Technology 13:1458–1465
    [Google Scholar]
  35. Van Dun C. M. P., Bol J. F. 1988; Transgenic tobacco plants accumulating tobacco rattle virus coat protein resist infection with tobacco rattle virus and pea early browning virus. Virology 167:649–652
    [Google Scholar]
  36. Van Dun C. M. P., Overduin B., Van Vloten-Doting L., Bol J. F. 1988; Transgenic tobacco expressing tobacco streak virus or mutated alfalfa mosaic virus coat protein does not cross-protect against alfalfa mosaic virus infection. Virology 164:383–389
    [Google Scholar]
  37. Wintermantel W. M., Schoelz J. E. 1996; Isolation of recombinant virus between cauliflower mosaic virus and a viral gene in transgenic plants under conditions of moderate selection pressure. Virology 223:156–164
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.18673-0
Loading
/content/journal/jgv/10.1099/vir.0.18673-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error