1887

Abstract

During the last 12 years, a strain of foot-and-mouth disease (FMD) virus serotype O, named PanAsia, has spread from India throughout Southern Asia and the Middle East. During 2000, this strain caused outbreaks in the Republic of Korea, Japan, Russia (Primorsky Territory), Mongolia and South Africa (KwaZulu-Natal Province), areas which last experienced FMD outbreaks in 1934, 1908, 1964, 1974 and 1957, respectively. In February 2001, the PanAsia strain spread to the United Kingdom where, in just over 7 months, it caused outbreaks on 2030 farms. From the UK, it quickly spread to the Republic of Ireland, France and the Netherlands. Previous studies that utilized RT-PCR to sequence the VP1-coding region of the RNA genomes of approximately 30 PanAsia isolates demonstrated that the UK virus was most closely related to the virus from South Africa (99·7 % nucleotide identity). To determine if there was an obvious genetic reason for the apparently high level of fitness of this new strain, and to further analyse the relationships between the PanAsia viruses and other FMDVs, complete genomes were amplified using long-range PCR techniques and the PCR products were sequenced, revealing the sequences for the entire genomes of five PanAsia isolates as well as an animal-passaged derivative of one of them. These genomes were compared to two other PanAsia genomes. These analyses revealed that all portions of the genomes of these isolates are highly conserved and provided confirmation of the close relationship between the viruses responsible for the South Africa and UK outbreaks, but failed to identify any genetic characteristic that could account for the unprecedented spread of this strain.

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2003-06-01
2020-01-29
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References

  1. Beard, C. W. & Mason, P. W. ( 2000; ). Genetic determinants of altered virulence of Taiwanese foot-and-mouth disease virus. J Virol 74, 987–991.[CrossRef]
    [Google Scholar]
  2. Clarke, B. E., Brown, A. L., Currey, K. M., Newton, S. E., Rowlands, D. J. & Carroll, A. R. ( 1987; ). Potential secondary and tertiary structure in the genomic RNA of foot and mouth disease virus. Nucleic Acids Res 15, 7067–7079.[CrossRef]
    [Google Scholar]
  3. Efron, B., Halloran, E. & Holmes, S. ( 1996; ). Bootstrap confidence levels for phylogenetic trees. Proc Natl Acad Sci U S A 93, 13429–13434.[CrossRef]
    [Google Scholar]
  4. Escarmis, C., Toja, M., Medina, M. & Domingo, E. ( 1992; ). Modifications of the 5′-untranslated region of foot-and-mouth disease virus after prolonged persistence in cell culture. Virus Res 26, 113–125.[CrossRef]
    [Google Scholar]
  5. Escarmis, C., Dopazo, J., Davila, M., Palma, E. L. & Domingo, E. ( 1995; ). Large deletions in the 5′-untranslated region of foot-and-mouth disease virus of serotype C. Virus Res 35, 155–167.[CrossRef]
    [Google Scholar]
  6. Feigelstock, D. A., Mateu, M. G., Valero, M. L., Andreu, D., Domingo, E. & Palma, E. L. ( 1996; ). Emerging foot-and-mouth disease virus variants with antigenically critical amino acid substitutions predicted by model studies using reference viruses. Vaccine 14, 97–102.[CrossRef]
    [Google Scholar]
  7. Frohman, M. A., Dush, M. K. & Martin, G. R. ( 1988; ). Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci U S A 85, 8998–9002.[CrossRef]
    [Google Scholar]
  8. Harris, T. J. ( 1980; ). Comparison of the nucleotide sequence at the 5′ end of RNAs from nine aphthoviruses, including representatives of the seven serotypes. J Virol 36, 659–664.
    [Google Scholar]
  9. Kanno, T., Yamakawa, M., Yoshida, K. & Sakamoto, K. ( 2002; ). The complete nucleotide sequence of the PanAsia strain of foot-and-mouth disease virus isolated in Japan. Virus Genes 25, 119–125.[CrossRef]
    [Google Scholar]
  10. Knowles, N. J., Davies, P. R., Henry, T., O'Donnell, V., Pacheco, J. M. & Mason, P. W. ( 2001a; ). Emergence in Asia of foot-and-mouth disease viruses with altered host range: characterization of alterations in the 3A protein. J Virol 75, 1551–1556.[CrossRef]
    [Google Scholar]
  11. Knowles, N. J., Samuel, A. R., Davies, P. R., Kitching, R. P. & Donaldson, A. I. ( 2001b; ). Outbreak of foot-and-mouth disease virus serotype O in the UK caused by a pandemic strain. Vet Rec 148, 258–259.
    [Google Scholar]
  12. Mason, P. W., Bezborodova, S. V. & Henry, T. M. ( 2002; ). Identification and characterization of a cis-acting replication element (cre) adjacent to the internal ribosome entry site of foot-and-mouth disease virus. J Virol 76, 9686–9694.[CrossRef]
    [Google Scholar]
  13. Martinez-Salas, E., Saiz, J. C., Davila, M., Belsham, G. J. & Domingo, E. ( 1993; ). A single nucleotide substitution in the internal ribosome entry site of foot-and-mouth disease virus leads to enhanced cap-independent translation in vivo. J Virol 67, 3748–3755.
    [Google Scholar]
  14. Mathews, D. H., Sabina, J., Zuker, M. & Turner, D. H. ( 1999; ). Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure. J Mol Biol 288, 911–940.[CrossRef]
    [Google Scholar]
  15. Núñez, J. I., Baranowski, E., Molina, N., Ruiz-Jarabo, C. M., Sánchez, C., Domingo, E. & Sobrino, F. ( 2001; ). A single amino acid substitution in nonstructural protein 3A can mediate adaptation of foot-and-mouth disease virus to the guinea pig. J Virol 75, 3977–3983.[CrossRef]
    [Google Scholar]
  16. Page, R. D. ( 1996; ). treeview: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  17. Pilipenko, E. V., Blinov, V. M., Chernov, B. K., Dmitrieva, T. M. & Agol, V. I. ( 1989; ). Conservation of the secondary structure elements of the 5′-untranslated region of cardio- and aphthovirus RNAs. Nucleic Acids Res 17, 5701–5711.[CrossRef]
    [Google Scholar]
  18. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  19. Samuel, A. R. & Knowles, N. J. ( 2001; ). Foot-and-mouth disease type O viruses exhibit genetically and geographically distinct evolutionary lineages (topotypes). J Gen Virol 82, 609–621.
    [Google Scholar]
  20. Tellier, R., Bukh, J., Emerson, S. U. & Purcell, R. H. ( 1996; ). Amplification of the full-length hepatitis A virus genome by long reverse transcription-PCR and transcription of infectious RNA directly from the amplicon. Proc Natl Acad Sci U S A 93, 4370–4373.[CrossRef]
    [Google Scholar]
  21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  22. Zuker, M., Mathews, D. H. & Turner, D. H. ( 1999; ). Algorithms and thermodynamics for RNA secondary structure prediction: a practical guide. In RNA Biochemistry and Biotechnology, pp. 11–43. Edited by J. Barciszewski & B. F. C. Clark. Dordrecht: Kluwer Academic Publishers.
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Supplements

*PCR indicates primers used for PCR (also RT, in the case of antisense primers). SEQ indicates primers used for sequencing only. Not all primers were utilized for all genomes. †Primer's sense on the genome. ‡sequence of the oligonucleotide, 5´ to 3´, standard abbreviations, lowercase bases do not match genomes but were needed for cDNA manipulations for other purposes. §Functional element followed by nucleotide numbers, in parentheses for the PanAsia genome numbered as shown in Fig.1 of the printed manuscript. ¦¦Also known as L-463F [#260, Samuel (2001)]. ¶Also known as O-1C564 [#260, Samuel (2001)]. #Also known as NK61 [#260, Samuel (2001)].

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Genome scanning comparisons of genomic sequence data of O/UKG/35/2001 versus FMDV A, C, Asia1 and SAT2 isolates. The origins of the sequence data are in the text and figure legends of the printed manuscript.

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