@article{mbs:/content/journal/jgv/10.1099/vir.0.18663-0, author = "Van de Walle, Gerlinde R. and Favoreel, Herman W. and Nauwynck, Hans J. and Pensaert, Maurice B.", title = "Antibody-induced internalization of viral glycoproteins and gE–gI Fc receptor activity protect pseudorabies virus-infected monocytes from efficient complement-mediated lysis", journal= "Journal of General Virology", year = "2003", volume = "84", number = "4", pages = "939-947", doi = "https://doi.org/10.1099/vir.0.18663-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.18663-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Pseudorabies virus (PRV)-infected blood monocytes are able to transport virus throughout the body of vaccination-immune pigs. PRV-infected monocytes express viral glycoproteins in their plasma membrane that can be recognized by virus-specific antibodies. Recently, it has been shown that addition of PRV-specific polyclonal immunoglobulins to PRV-infected monocytes at 37 °C induces internalization of the majority of plasma membrane-expressed viral glycoproteins. This study investigated whether this process may interfere with efficient antibody-dependent complement-mediated lysis (ADCML) of infected monocytes. Therefore, an ADCML assay was set up in vitro. A significant decrease in the percentage of cells lysed by ADCML was observed when antibody-induced internalization of PRV glycoproteins occurred (P<0·005). Furthermore, it is shown (i) that the PRV gE–gI complex, which, like certain other alphaherpesvirus orthologues, possesses IgG-binding capacity, aids in avoiding efficient ADCML of PRV-infected monocytes and (ii) that the efficiency of PRV gE–gI-mediated evasion of ADCML can be decreased by the presence of gE–gI-specific antibodies.", }