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Eighteen samples of begomoviruses isolated from tobacco, tomato and weed species in Yunnan, China were found to be associated with DNAβ molecules, for which the complete nucleotide sequences were found to contain 1333–1355 nt. The 18 DNAβ molecules identified consist of three main types, each associated with a different begomovirus species: 72–99 % nucleotide identity was found within one type, but only 39–57 % identity was found between types. All the DNAβ molecules reported here and elsewhere contain a 115 nt conserved region that has 93–100 % identity with a consensus sequence, and have a common ORF encoding 118 amino acids on the complementary strand (designated C1). Co-agroinoculation of the DNA-A component of Tomato yellow leaf curl China virus tobacco isolate Y10, with its associated DNAβ (Y10β), shows this DNAβ to be involved in symptom induction in tobacco and tomato. The in-frame ATG mutation of C1 of Y10β caused much milder symptoms as compared with wild Y10β, indicating a functional role for this ORF. Pairwise nucleotide sequence identity comparisons of DNAβ molecules and their cognate viral DNA-A molecules indicate that DNAβ molecules have co-evolved with their cognate helper viruses. Recombination between DNAβ molecules is documented and a DNAβ species concept is proposed and discussed.
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