@article{mbs:/content/journal/jgv/10.1099/vir.0.18478-0, author = "Mateu, Enric and Martín, Marga and Vidal, Dolors", title = "Genetic diversity and phylogenetic analysis of glycoprotein 5 of European-type porcine reproductive and respiratory virus strains in Spain", journal= "Journal of General Virology", year = "2003", volume = "84", number = "3", pages = "529-534", doi = "https://doi.org/10.1099/vir.0.18478-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.18478-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The gene encoding glycoprotein 5 (ORF5) of 21 porcine reproductive and respiratory syndrome virus (PRRSV) isolates from Spain and two European-type vaccines currently available in that country were analysed using RT-PCR and sequencing. Sequences were then compared with other European-type sequences available through GenBank. Results showed percentages of similarity to Lelystad virus (LV), which, in most cases, were below 90 %. In contrast, two strains were very similar (>99 %) to a PRRSV variant from the Czech Republic. Evolutionary trees showed three types of strains: one grouped old Spanish sequences; a second grouped isolates from this study together with two Czech variant strains; and the third comprised other GenBank sequences. Regarding the predicted protein sequences, some isolates from this study showed a low degree of similarity to LV (below 50 %) and most of the strains examined had additional N-linked glycosylation sites compared to LV. These results provide evidence of the existence of variant PRRSV strains in Spain with characteristics that may be advantageous for immune evasion.", }