RT Journal Article SR Electronic(1) A1 Tanzi, Elisabetta A1 Bianchi, Silvia A1 Frati, Elena R. A1 Amicizia, Daniela A1 Martinelli, Marianna A1 Bragazzi, Nicola L. A1 Brisigotti, Maria Pia A1 Colzani, Daniela A1 Fasoli, Ester A1 Zehender, Gianguglielmo A1 Panatto, Donatella A1 Gasparini, RobertoYR 2015 T1 Human papillomavirus detection in paraffin-embedded colorectal cancer tissues JF Journal of General Virology, VO 96 IS 1 SP 206 OP 209 DO https://doi.org/10.1099/vir.0.070672-0 PB Microbiology Society, SN 1465-2099, AB Human papillomavirus (HPV) has a well-recognized aetiological role in the development of cervical cancer and other anogenital tumours. Recently, an association between colorectal cancer and HPV infection has been suggested, although this is still controversial. This study aimed at detecting and characterizing HPV infection in 57 paired biopsies from colorectal cancers and adjacent intact tissues using a degenerate PCR approach. All amplified fragments were genotyped by means of sequencing. Overall, HPV prevalence was 12.3 %. In particular, 15.8 % of tumour tissues and 8.8 % of non-cancerous tissue samples were HPV DNA-positive. Of these samples, 85.7 % were genotyped successfully, with 41.7 % of sequences identifying four genotypes of the HR (high oncogenic risk) clade Group 1; the remaining 58.3 % of HPV-genotyped specimens had an unclassified β-HPV. Examining additional cases and analysing whole genomes will help to outline the significance of these findings., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.070672-0