RT Journal Article SR Electronic(1) A1 Zhang, Qingli A1 Liu, Qun A1 Liu, Shuang A1 Yang, Haolin A1 Liu, Sun A1 Zhu, Luoluo A1 Yang, Bing A1 Jin, Jiting A1 Ding, Lixue A1 Wang, Xiuhua A1 Liang, Yan A1 Wang, Qintao A1 Huang, JieYR 2014 T1 A new nodavirus is associated with covert mortality disease of shrimp JF Journal of General Virology, VO 95 IS 12 SP 2700 OP 2709 DO https://doi.org/10.1099/vir.0.070078-0 PB Microbiology Society, SN 1465-2099, AB A new nodavirus, named covert mortality nodavirus (CMNV), is associated with covert mortality disease of shrimp which has caused serious loss in China since 2009. Histopathological examination of shrimp suffering the disease revealed coagulative necrosis of striated muscle similar to typical histopathology features of infectious myonecrosis virus (IMNV), Penaeus vannamei nodavirus (PvNV) and Macrobrachium rosenbergii nodavirus (MrNV). However, shrimp suffering this disease tested negative for IMNV, MrNV and PvNV by reverse transcription (RT)-PCR. Additionally, eosinophilic inclusions were found in epithelium of the tubules in the hepatopancreas and lymphoid organ, and mass karyopyknotic nuclei existed in the muscle and lymphoid organ. The tubular epithelium of the hepatopancreas showed significant atrophy. A cDNA library was constructed from total RNA of infected shrimp. Sequencing and alignment analysis showed that one clone with an 1185 bp insert (designated CMNV-7) shared 54 , 53  and 39 % identity with the amino acid sequences of RNA-dependent RNA polymerase from Flock House virus, black beetle virus and MrNV. The results of fluorescence in situ hybridization showed that the hepatopancreas, striated muscle and lymphoid organ were positively reacting tissues. The mean size of negative-stained virus particles was 32 nm. In addition, a nested RT-PCR assay was developed for CMNV, and the RT-PCR detection results revealed that Fenneropenaeus chinensis, Litopenaeus vannamei and Marsupenaeus japonicus suffering from this disease were CMNV-positive., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.070078-0