1887

Abstract

Newcastle disease virus (NDV) has been developed as a vector for vaccine and gene therapy purposes. However, the optimal insertion site for foreign gene expression remained to be determined. In the present study, we inserted the green fluorescence protein (GFP) gene into five different intergenic regions of the enterotropic NDV VG/GA vaccine strain using reverse genetics technology. The rescued recombinant viruses retained lentogenic pathotype and displayed delayed growth dynamics, particularly when the GFP gene was inserted between the NP and P genes of the virus. The GFP mRNA level was most abundant when the gene was inserted closer to the 3′ end and gradually decreased as the gene was inserted closer to the 5′ end. Measurement of the GFP fluorescence intensity in recombinant virus-infected cells demonstrated that the non-coding region between the P and M genes is the optimal insertion site for foreign gene expression in the VG/GA vaccine vector.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.068437-0
2015-01-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/96/1/40.html?itemId=/content/journal/jgv/10.1099/vir.0.068437-0&mimeType=html&fmt=ahah

References

  1. Alexander D. J. 1998; Newcastle disease virus and other avian paramyxoviruses. In A Laboratory Manual for the Isolation and Identification of Avian Pathogens, 4th edn. pp. 156–163 Edited by Swayne D., Glisson J. R., Jackwood M. W., Pearson J. E., Reed W.M. Kennett Square, PA: American Association of Avian Pathologists;
    [Google Scholar]
  2. Beard C. W., Villegas P., Glisson J. R. 1993; Comparative efficacy of the B-1 and VG/GA vaccine strains against velogenic viscerotropic Newcastle disease virus in chickens. Avian Dis 37:222–225 [View Article][PubMed]
    [Google Scholar]
  3. Bukreyev A., Collins P. L. 2008; Newcastle disease virus as a vaccine vector for humans. Curr Opin Mol Ther 10:46–55[PubMed]
    [Google Scholar]
  4. Bukreyev A., Huang Z., Yang L., Elankumaran S., St Claire M., Murphy B. R., Samal S. K., Collins P. L. 2005; Recombinant Newcastle disease virus expressing a foreign viral antigen is attenuated and highly immunogenic in primates. J Virol 79:13275–13284 [View Article][PubMed]
    [Google Scholar]
  5. Carnero E., Li W., Borderia A. V., Moltedo B., Moran T., García-Sastre A. 2009; Optimization of human immunodeficiency virus gag expression by Newcastle disease virus vectors for the induction of potent immune responses. J Virol 83:584–597 [View Article][PubMed]
    [Google Scholar]
  6. de Leeuw O., Peeters B. 1999; Complete nucleotide sequence of Newcastle disease virus: evidence for the existence of a new genus within the subfamily Paramyxovirinae. J Gen Virol 80:131–136[PubMed]
    [Google Scholar]
  7. DiNapoli J. M., Kotelkin A., Yang L., Elankumaran S., Murphy B. R., Samal S. K., Collins P. L., Bukreyev A. 2007; Newcastle disease virus, a host range-restricted virus, as a vaccine vector for intranasal immunization against emerging pathogens. Proc Natl Acad Sci U S A 104:9788–9793 [View Article][PubMed]
    [Google Scholar]
  8. Hu H., Roth J. P., Estevez C. N., Zsak L., Liu B., Yu Q. 2011; Generation and evaluation of a recombinant Newcastle disease virus expressing the glycoprotein (G) of avian metapneumovirus subgroup C as a bivalent vaccine in turkeys. Vaccine 29:8624–8633 [View Article][PubMed]
    [Google Scholar]
  9. Huang Z., Elankumaran S., Panda A., Samal S. K. 2003; Recombinant Newcastle disease virus as a vaccine vector. Poult Sci 82:899–906 [View Article][PubMed]
    [Google Scholar]
  10. Huang Z., Elankumaran S., Yunus A. S., Samal S. K. 2004; A recombinant Newcastle disease virus (NDV) expressing VP2 protein of infectious bursal disease virus (IBDV) protects against NDV and IBDV. J Virol 78:10054–10063 [View Article][PubMed]
    [Google Scholar]
  11. Lamb R. A., Parks G. D. 2007; Paramyxoviridae: the viruses and their replication. In Fields Virology, 5th edn. pp. 1440–1496 Edited by Knipe D. M., Howley P. M. Philadelphia, PA: Lippincott Williams & Wilkins;
    [Google Scholar]
  12. Lamb R. A., Collins P. L., Kolakofsky D., Melero J. A., Nagai Y., Oldstone M. B. A., Pringle C. R., Rima B. K. 2005; Family Paramyxoviridae. In Virus Taxonomy, Eighth Report of the International Committee on Taxonomy of Viruses pp. 655–668 Edited by Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball L. A. San Diego, CA: Elsevier Academic Press;
    [Google Scholar]
  13. Le Nouën C., Munir S., Losq S., Winter C. C., McCarty T., Stephany D. A., Holmes K. L., Bukreyev A., Rabin R. L.other authors 2009; Infection and maturation of monocyte-derived human dendritic cells by human respiratory syncytial virus, human metapneumovirus, and human parainfluenza virus type 3. Virology 385:169–182 [View Article][PubMed]
    [Google Scholar]
  14. Miller P. J., Guus K. 2013; Newcastle Disease. In Diseases of Poultry, 13th edn. pp. 98–107 Edited by Swayne D. E., Glisson J. R., McDougald L. R., Nolan L. K., Suarez D. L., Nair V. Ames, IA: Wiley-Blackwell;
    [Google Scholar]
  15. Park M. S., Steel J., García-Sastre A., Swayne D., Palese P. 2006; Engineered viral vaccine constructs with dual specificity: avian influenza and Newcastle disease. Proc Natl Acad Sci U S A 103:8203–8208 [View Article][PubMed]
    [Google Scholar]
  16. Pattnaik A. K., Wertz G. W. 1990; Replication and amplification of defective interfering particle RNAs of vesicular stomatitis virus in cellsexpressing viral proteins from vectors containing cloned cDNAs. JVirol 64:2948–2957[PubMed]
    [Google Scholar]
  17. Peeters B. P., de Leeuw O. S., Koch G., Gielkens A. L. 1999; Rescue of Newcastle disease virus from cloned cDNA: evidence that cleavability of the fusion protein is a major determinant for virulence. J Virol 73:5001–5009[PubMed]
    [Google Scholar]
  18. Peeters B. P., Gruijthuijsen Y. K., de Leeuw O. S., Gielkens A. L. 2000; Genome replication of Newcastle disease virus: involvement of the rule-of-six. Arch Virol 145:1829–1845 [View Article][PubMed]
    [Google Scholar]
  19. Perozo F., Villegas P., Afonso C. L. 2008a; Genomic comparison of the complete coding and intergenic regions of the VG/GA Newcastle disease virus and its respirotropic clone 5. Virus Genes 37:161–167 [View Article][PubMed]
    [Google Scholar]
  20. Perozo F., Villegas P., Dolz R., Afonso C. L., Purvis L. B. 2008b; The VG/GA strain of Newcastle disease virus: mucosal immunity, protection against lethal challenge and molecular analysis. Avian Pathol 37:237–245 [View Article][PubMed]
    [Google Scholar]
  21. Ramp K., Skiba M., Karger A., Mettenleiter T. C., Römer-Oberdörfer A. 2011; Influence of insertion site of the avian influenza virus haemagglutinin (HA) gene within the Newcastle disease virus genome on HA expression. J Gen Virol 92:355–360 [View Article][PubMed]
    [Google Scholar]
  22. Schirrmacher V., Fournier P. 2009; Newcastle disease virus: a promising vector for viral therapy, immune therapy, and gene therapy of cancer. Methods Mol Biol 542:565–605 [View Article][PubMed]
    [Google Scholar]
  23. Skiadopoulos M. H., Surman S. R., Durbin A. P., Collins P. L., Murphy B. R. 2000; Long nucleotide insertions between the HN and L protein coding regions of human parainfluenza virus type 3 yield viruses with temperature-sensitive and attenuation phenotypes. Virology 272:225–234 [View Article][PubMed]
    [Google Scholar]
  24. Vigil A., Martinez O., Chua M. A., García-Sastre A. 2008; Recombinant Newcastle disease virus as a vaccine vector for cancer therapy. Mol Ther 16:1883–1890 [View Article][PubMed]
    [Google Scholar]
  25. Wertz G. W., Moudy R., Ball L. A. 2002; Adding genes to the RNA genome of vesicular stomatitis virus: positional effects on stability of expression. J Virol 76:7642–7650 [View Article][PubMed]
    [Google Scholar]
  26. Yu Q., Roth J. P., Hu H., Estevez C. N., Zhao W., Zsak L. 2013; Protection by recombinant Newcastle disease viruses (NDV) expressing the glycoprotein (G) of avian metapneumovirus (aMPV) subtype A or B against challenge with virulent NDV and aMPV. World Journal of Vaccines 3:130–139 [View Article]
    [Google Scholar]
  27. Zhao H., Peeters B. P. 2003; Recombinant Newcastle disease virus as a viral vector: effect of genomic location of foreign gene on gene expression and virus replication. J Gen Virol 84:781–788 [View Article][PubMed]
    [Google Scholar]
  28. Zhao W., Spatz S., Zhang Z., Wen G., Garcia M., Zsak L., Yu Q. 2014; Newcastle disease virus (NDV) recombinants expressing infectious laryngotracheitis virus (ILTV) glycoproteins gB and gD protect chickens against ILTV and NDV challenges. J Virol 88:8397–8406 [View Article][PubMed]
    [Google Scholar]
/content/journal/jgv/10.1099/vir.0.068437-0
Loading
/content/journal/jgv/10.1099/vir.0.068437-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error