1887

Abstract

During a hospital-based diarrhoeal disease study conducted in Ho Chi Minh City, Vietnam from 2009 to 2010, we identified four symptomatic children infected with G26P[19] rotavirus (RV) – an atypical variant that has not previously been reported in human gastroenteritis. To determine the genetic structure and investigate the origin of this G26P[19] strain, the whole genome of a representative example was characterized, revealing a novel genome constellation: G26–P[19]–I5–R1–C1–M1–A8–N1–T1–E1–H1. The genome segments were most closely related to porcine (VP7, VP4, VP6 and NSP1) and Wa-like porcine RVs (VP1–3 and NSP2–5). We proposed that this G26P[19] strain was the product of zoonotic transmission coupled with one or more reassortment events occurring in human and/or animal reservoirs. The identification of such strains has potential implications for vaccine efficacy in south-east Asia, and outlines the utility of whole-genome sequencing for studying RV diversity and zoonotic potential during disease surveillance.

  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.068403-0
2014-12-01
2022-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/95/12/2727.html?itemId=/content/journal/jgv/10.1099/vir.0.068403-0&mimeType=html&fmt=ahah

References

  1. Abe M., Ito N., Masatani T., Nakagawa K., Yamaoka S., Kanamaru Y., Suzuki H., Shibano K., Arashi Y., Sugiyama M. 2011; Whole genome characterization of new bovine rotavirus G21P[29] and G24P[33] strains provides evidence for interspecies transmission. J Gen Virol 92:952–960 [View Article][PubMed]
    [Google Scholar]
  2. Bernstein D. I. 2009; Rotavirus overview. Pediatr Infect Dis J 28:S50–S53 [View Article][PubMed]
    [Google Scholar]
  3. Burke B., McCrae M. A., Desselberger U. 1994; Sequence analysis of two porcine rotaviruses differing in growth in vitro and in pathogenicity: distinct VP4 sequences and conservation of NS53, VP6 and VP7 genes. J Gen Virol 75:2205–2212 [View Article][PubMed]
    [Google Scholar]
  4. Chitambar S. D., Arora R., Chhabra P. 2009; Molecular characterization of a rare G1P[19] rotavirus strain from India: evidence of reassortment between human and porcine rotavirus strains. J Med Microbiol 58:1611–1615 [View Article][PubMed]
    [Google Scholar]
  5. Cook N., Bridger J., Kendall K., Gomara M. I., El-Attar L., Gray J. 2004; The zoonotic potential of rotavirus. J Infect 48:289–302 [View Article][PubMed]
    [Google Scholar]
  6. Cortese M. M., Parashar U. D. Centers for Disease Control and Prevention (CDC) 2009; Prevention of Rotavirus Gastroenteritis Among Infants and Children: recommendations of the Advisory Committee on Immunization Practices (ACIP). MMWR Morb Mortal Wkly Rep 58:1–25[PubMed]
    [Google Scholar]
  7. Cowley D., Donato C. M., Roczo-Farkas S., Kirkwood C. D. 2013; Novel G10P[14] rotavirus strain, northern territory, Australia. Emerg Infect Dis 19:1324–1327 [View Article][PubMed]
    [Google Scholar]
  8. Estes M. K., Kapikian A. Z., Knipe D. M., Howley P. M. 2007; Rotaviruses. In Fields Virology, 5th edn. pp. 1917–1974 Edited by Knipe D. M., Howley P. M. Philadelphia, PA: Lippincott Williams & Wilkins;
    [Google Scholar]
  9. Ghosh S., Urushibara N., Taniguchi K., Kobayashi N. 2012; Whole genomic analysis reveals the porcine origin of human G9P[19] rotavirus strains Mc323 and Mc345. Infect Genet Evol 12:471–477 [View Article][PubMed]
    [Google Scholar]
  10. Glass R. I., Parashar U. D., Bresee J. S., Turcios R., Fischer T. K., Widdowson M.-A., Jiang B., Gentsch J. R. 2006; Rotavirus vaccines: current prospects and future challenges. Lancet 368:323–332 [View Article][PubMed]
    [Google Scholar]
  11. Guindon S., Dufayard J.-F., Lefort V., Anisimova M., Hordijk W., Gascuel O. 2010; New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59:307–321 [View Article][PubMed]
    [Google Scholar]
  12. Guo D., Liu J., Lu Y., Sun Y., Yuan D., Jiang Q., Lin H., Li C., Si C., Qu L. 2012; Full genomic analysis of rabbit rotavirus G3P[14] strain N5 in China: identification of a novel VP6 genotype. Infect Genet Evol 12:1567–1576 [View Article][PubMed]
    [Google Scholar]
  13. Jere K. C., Esona M. D., Ali Y. H., Peenze I., Roy S., Bowen M. D., Saeed I. K., Khalafalla A. I., Nyaga M. M. other authors 2014; Novel NSP1 genotype characterised in an African camel G8P[11] rotavirus strain. Infect Genet Evol 21:58–66 [View Article][PubMed]
    [Google Scholar]
  14. Krishnan T., Burke B., Shen S., Naik T. N., Desselberger U. 1994; Molecular epidemiology of human rotaviruses in Manipur: genome analysis of rotaviruses of long electropherotype and subgroup I. Arch Virol 134:279–292 [View Article][PubMed]
    [Google Scholar]
  15. Maes P., Matthijnssens J., Rahman M., Van Ranst M. 2009; RotaC: a web-based tool for the complete genome classification of group A rotaviruses. BMC Microbiol 9:238 [View Article][PubMed]
    [Google Scholar]
  16. Maneekarn N., Khamrin P., Chan-it W., Peerakome S., Sukchai S., Pringprao K., Ushijima H. 2006; Detection of rare G3P[19] porcine rotavirus strains in Chiang Mai, Thailand, provides evidence for origin of the VP4 genes of Mc323 and Mc345 human rotaviruses. J Clin Microbiol 44:4113–4119 [View Article][PubMed]
    [Google Scholar]
  17. Matthijnssens J., Ciarlet M., McDonald S. M., Attoui H., Bányai K., Brister J. R., Buesa J., Esona M. D., Estes M. K. other authors 2011; Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG). Arch Virol 156:1397–1413 [View Article][PubMed]
    [Google Scholar]
  18. Miyazaki A., Kuga K., Suzuki T., Kohmoto M., Katsuda K., Tsunemitsu H. 2011; Genetic diversity of group A rotaviruses associated with repeated outbreaks of diarrhea in a farrow-to-finish farm: identification of a porcine rotavirus strain bearing a novel VP7 genotype, G26. Vet Res 42:112 [View Article][PubMed]
    [Google Scholar]
  19. Mukherjee A., Ghosh S., Bagchi P., Dutta D., Chattopadhyay S., Kobayashi N., Chawla-Sarkar M. 2011; Full genomic analyses of human rotavirus G4P[4], G4P[6], G9P[19] and G10P[6] strains from North-eastern India: evidence for interspecies transmission and complex reassortment events. Clin Microbiol Infect 17:1343–1346[PubMed] [CrossRef]
    [Google Scholar]
  20. My P. V. T., Rabaa M. A., Vinh H., Holmes E. C., Hoang N. V., Vinh N. T., Phuong T., Tham N. T., Bay P. V. other authors 2011; The emergence of rotavirus G12 and the prevalence of enteric viruses in hospitalized pediatric diarrheal patients in southern Vietnam. Am J Trop Med Hyg 85:768–775 [View Article][PubMed]
    [Google Scholar]
  21. My P. V. T., Thompson C., Phuc H. L., Tuyet P. T. N., Vinh H., Noang N. V. M., Minh P. V., Vinh N. T., Thuy C. T. other authors 2013; Endemic norovirus infections in children, Ho Chi Minh City, Vietnam, 2009–2010. Emerg Infect Dis 19:977–980 [View Article][PubMed]
    [Google Scholar]
  22. Nguyen T. A., Hoang L. P., Pham L. D., Hoang K. T., Okitsu S., Mizuguchi M., Ushijima H. 2008; Use of sequence analysis of the VP4 gene to classify recent Vietnamese rotavirus isolates. Clin Microbiol Infect 14:235–241 [View Article][PubMed]
    [Google Scholar]
  23. Okada J., Urasawa T., Kobayashi N., Taniguchi K., Hasegawa A., Mise K., Urasawa S. 2000; New P serotype of group A human rotavirus closely related to that of a porcine rotavirus. J Med Virol 60:63–69 [View Article][PubMed]
    [Google Scholar]
  24. Papp H., Al-Mutairi L. Z., Chehadeh W., Farkas S. L., Lengyel G., Jakab F., Martella V., Szűcs G., Bányai K. 2012; Novel NSP4 genotype in a camel G10P[15] rotavirus strain. Acta Microbiol Immunol Hung 59:411–421 [View Article][PubMed]
    [Google Scholar]
  25. Parashar U. D., Bresee J. S., Glass R. I. 2003; The global burden of diarrhoeal disease in children. Bull World Health Organ 81:236[PubMed]
    [Google Scholar]
  26. Parashar U. D., Gibson C. J., Bresee J. S., Glass R. I. 2006; Rotavirus and severe childhood diarrhea. Emerg Infect Dis 12:304–306 [View Article][PubMed]
    [Google Scholar]
  27. Pond S. L., Frost S. D., Muse S. V. 2005; HyPhy: hypothesis testing using phylogenies. Bioinformatics 21:676–679 [View Article][PubMed]
    [Google Scholar]
  28. Posada D., Crandall K. A. 1998; modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818 [View Article][PubMed]
    [Google Scholar]
  29. Santos N., Hoshino Y. 2005; Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. Rev Med Virol 15:29–56 [View Article][PubMed]
    [Google Scholar]
  30. Tate J. E., Patel M. M., Cortese M. M., Lopman B. A., Gentsch J. R., Fleming J., Steele A. D., Parashar U. D. 2012; Remaining issues and challenges for rotavirus vaccine in preventing global childhood diarrheal morbidity and mortality. Expert Rev Vaccines 11:211–220 [View Article][PubMed]
    [Google Scholar]
  31. Theamboonlers A., Bhattarakosol P., Chongsrisawat V., Sungkapalee T., Wutthirattanakowit N., Poovorawan Y. 2008; Molecular characterization of group A human rotaviruses in Bangkok and Buriram, Thailand during 2004-2006 reveals the predominance of G1P[8], G9P[8] and a rare G3P[19] strain. Virus Genes 36:289–298 [View Article][PubMed]
    [Google Scholar]
  32. Trojnar E., Sachsenröder J., Twardziok S., Reetz J., Otto P. H., Johne R. 2013; Identification of an avian group A rotavirus containing a novel VP4 gene with a close relationship to those of mammalian rotaviruses. J Gen Virol 94:136–142 [View Article][PubMed]
    [Google Scholar]
  33. Urasawa S., Hasegawa A., Urasawa T., Taniguchi K., Wakasugi F., Suzuki H., Inouye S., Pongprot B., Supawadee J. other authors 1992; Antigenic and genetic analyses of human rotaviruses in Chiang Mai, Thailand: evidence for a close relationship between human and animal rotaviruses. J Infect Dis 166:227–234 [View Article][PubMed]
    [Google Scholar]
  34. Varghese V., Das S., Singh N. B., Kojima K., Bhattacharya S. K., Krishnan T., Kobayashi N., Naik T. N. 2004; Molecular characterization of a human rotavirus reveals porcine characteristics in most of the genes including VP6 and NSP4. Arch Virol 149:155–172 [View Article][PubMed]
    [Google Scholar]
  35. Wu F. T., Bányai K., Huang J. C., Wu H. S., Chang F. Y., Yang J. Y., Hsiung C. A., Huang Y. C., Lin J. S. other authors 2011; Diverse origin of P[19] rotaviruses in children with acute diarrhea in Taiwan: Detection of novel lineages of the G3, G5, and G9 VP7 genes. J Med Virol 83:1279–1287 [View Article][PubMed]
    [Google Scholar]
  36. Zade J. K., Chhabra P., Chitambar S. D. 2009; Characterization of VP7 and VP4 genes of rotavirus strains: 1990–1994 and 2000–2002. Epidemiol Infect 137:936–942 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.068403-0
Loading
/content/journal/jgv/10.1099/vir.0.068403-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error