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Volume 95, Issue 12

Inhibition of the foot-and-mouth disease virus subgenomic replicon by RNA aptamers Open Access

Abstract

We have previously documented the inhibitory activity of RNA aptamers to the RNA-dependent RNA polymerase of foot-and-mouth disease virus (3D). Here we report their modification and use with a subgenomic replicon incorporating GFP (pGFP-PAC replicon), allowing replication to be monitored and quantified in real-time. GFP expression in transfected BHK-21 cells reached a maximum at approximately 8 h post-transfection, at which time change in morphology of the cells was consistent with a virus-induced cytopathic effect. However, transfection of replicon-bearing cells with a 3D aptamer RNA resulted in inhibition of GFP expression and maintenance of normal cell morphology, whereas a control aptamer RNA had little effect. The inhibition was correlated with a reduction in 3D (detected by immunoblotting) and shown to be dose dependent. The 3D aptamers appeared to be more effective than 2′--methylcytidine (2′CMC). Aptamers to components of the replication complex are therefore useful molecular tools for studying viral replication and also have potential as diagnostic molecules in the future.

  • Received:
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© 2014 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2014-12-01
2023-09-28
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Inhibition of the foot-and-mouth disease virus subgenomic replicon by RNA aptamers
J. Gen. Virol. 95, 2649 (2014); https://doi.org/10.1099/vir.0.067751-0
/content/journal/jgv/10.1099/vir.0.067751-0
/content/journal/jgv/10.1099/vir.0.067751-0
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