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Abstract

Porcine haemagglutinating encephalomyelitis virus (PHEV) is the main causative agent of porcine coronavirus-associated disease, which is characterized by encephalomyelitis and involves the central nervous system. Little is known about the molecular mechanisms of brain injury caused by PHEV. To gain insight into the interaction between the virus and host cells, changes in global gene expression in the cerebral cortex of PHEV- or mock-infected mice were investigated using DNA microarray analysis and quantitative real-time PCR. The results of the microarray analysis showed that 365 genes on day 3 post-infection (p.i.) and 781 genes on day 5 p.i. were differentially expressed in response to PHEV infection in the cerebral cortex. The upregulated genes were mainly involved in immune system processes, antigen processing and presentation, the Jak–STAT signalling pathway, the RIG-I-like receptor signalling pathway, Toll-like receptor signalling and apoptosis-related proteases. Significantly downregulated genes were mainly involved in nervous-system development, synaptic transmission, neuron-projection development, the transmission of nerve impulses and negative regulation of glial cell differentiation. The differential expression of these genes suggests a strong antiviral host response, but may also contribute to the pathogenesis of PHEV resulting in encephalomyelitis.

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2014-10-01
2024-03-29
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References

  1. Chakrabarti A. K., Vipat V. C., Mukherjee S., Singh R., Pawar S. D., Mishra A. C. 2010; Host gene expression profiling in influenza A virus-infected lung epithelial (A549) cells: a comparative analysis between highly pathogenic and modified H5N1 viruses. Virol J 7:219 [View Article][PubMed]
    [Google Scholar]
  2. Diamond M. S., Mehlhop E., Oliphant T., Samuel M. A. 2009; The host immunologic response to West Nile encephalitis virus. Front Biosci (Landmark Ed) 14:3024–3034 [View Article][PubMed]
    [Google Scholar]
  3. Fink J., Gu F., Ling L., Tolfvenstam T., Olfat F., Chin K. C., Aw P., George J., Kuznetsov V. A.other authors 2007; Host gene expression profiling of dengue virus infection in cell lines and patients. PLoS Negl Trop Dis 1:e86 [View Article][PubMed]
    [Google Scholar]
  4. Fredericksen B. L., Smith M., Katze M. G., Shi P. Y., Gale M. Jr 2004; The host response to West Nile virus infection limits viral spread through the activation of the interferon regulatory factor 3 pathway. J Virol 78:7737–7747 [View Article][PubMed]
    [Google Scholar]
  5. Gao W., Zhao K., Zhao C., Du C., Ren W., Song D., Lu H., Chen K., Li Z.other authors 2011; Vomiting and wasting disease associated with hemagglutinating encephalomyelitis viruses infection in piglets in Jilin, China. Virol J 8:130 [View Article][PubMed]
    [Google Scholar]
  6. Gladue D. P., Zhu J., Holinka L. G., Fernandez-Sainz I., Carrillo C., Prarat M. V., O’Donnell V., Borca M. V. 2010; Patterns of gene expression in swine macrophages infected with classical swine fever virus detected by microarray. Virus Res 151:10–18 [View Article][PubMed]
    [Google Scholar]
  7. Gupta N., Santhosh S. R., Babu J. P., Parida M. M., Rao P. V. 2010; Chemokine profiling of Japanese encephalitis virus-infected mouse neuroblastoma cells by microarray and real-time RT-PCR: implication in neuropathogenesis. Virus Res 147:107–112 [View Article][PubMed]
    [Google Scholar]
  8. Hara Y., Hasebe R., Sunden Y., Ochiai K., Honda E., Sakoda Y., Umemura T. 2009; Propagation of swine hemagglutinating encephalomyelitis virus and pseudorabies virus in dorsal root ganglia cells. J Vet Med Sci 71:595–601 [View Article][PubMed]
    [Google Scholar]
  9. Hirano N., Nomura R., Tawara T., Tohyama K. 2004; Neurotropism of swine haemagglutinating encephalomyelitis virus (coronavirus) in mice depending upon host age and route of infection. J Comp Pathol 130:58–65 [View Article][PubMed]
    [Google Scholar]
  10. Hsieh M. F., Lai S. L., Chen J. P., Sung J. M., Lin Y. L., Wu-Hsieh B. A., Gerard C., Luster A., Liao F. 2006; Both CXCR3 and CXCL10/IFN-inducible protein 10 are required for resistance to primary infection by dengue virus. J Immunol 177:1855–1863 [View Article][PubMed]
    [Google Scholar]
  11. Kajaste-Rudnitski A., Mashimo T., Frenkiel M. P., Guénet J. L., Lucas M., Desprès P. 2006; The 2′,5′-oligoadenylate synthetase 1b is a potent inhibitor of West Nile virus replication inside infected cells. J Biol Chem 281:4624–4637 [View Article][PubMed]
    [Google Scholar]
  12. Kash J. C., Tumpey T. M., Proll S. C., Carter V., Perwitasari O., Thomas M. J., Basler C. F., Palese P., Taubenberger J. K.other authors 2006; Genomic analysis of increased host immune and cell death responses induced by 1918 influenza virus. Nature 443:578–581[PubMed]
    [Google Scholar]
  13. Knapp S., Yee L. J., Frodsham A. J., Hennig B. J., Hellier S., Zhang L., Wright M., Chiaramonte M., Graves M.other authors 2003; Polymorphisms in interferon-induced genes and the outcome of hepatitis C virus infection: roles of MxA, OAS-1 and PKR. Genes Immun 4:411–419 [View Article][PubMed]
    [Google Scholar]
  14. Lan Y., Zhao K., Wang G., Dong B., Zhao J., Tang B., Lu H., Gao W., Chang L.other authors 2013; Porcine hemagglutinating encephalomyelitis virus induces apoptosis in a porcine kidney cell line via caspase-dependent pathways. Virus Res 176:292–297 [View Article][PubMed]
    [Google Scholar]
  15. Lee G., Han D., Song J. Y., Lee Y. S., Kang K. S., Yoon S. 2010; Genomic expression profiling in lymph nodes with lymphoid depletion from porcine circovirus 2-infected pigs. J Gen Virol 91:2585–2591 [View Article][PubMed]
    [Google Scholar]
  16. Lenschow D. J., Giannakopoulos N. V., Gunn L. J., Johnston C., O’Guin A. K., Schmidt R. E., Levine B., Virgin H. W. IV 2005; Identification of interferon-stimulated gene 15 as an antiviral molecule during Sindbis virus infection in vivo. J Virol 79:13974–13983 [View Article][PubMed]
    [Google Scholar]
  17. Li Y. C., Bai W. Z., Hirano N., Hayashida T., Taniguchi T., Sugita Y., Tohyama K., Hashikawa T. 2013; Neurotropic virus tracing suggests a membranous-coating-mediated mechanism for transsynaptic communication. J Comp Neurol 521:203–212 [View Article][PubMed]
    [Google Scholar]
  18. Martinon F., Tschopp J. 2007; Inflammatory caspases and inflammasomes: master switches of inflammation. Cell Death Differ 14:10–22 [View Article][PubMed]
    [Google Scholar]
  19. Masters P. S. 2006; The molecular biology of coronaviruses. Adv Virus Res 66:193–292 [View Article][PubMed]
    [Google Scholar]
  20. Mengeling W. L., Boothe A. D., Ritchie A. E. 1972; Characteristics of a coronavirus (strain 67N) of pigs. Am J Vet Res 33:297–308[PubMed]
    [Google Scholar]
  21. Ritchie K. J., Hahn C. S., Kim K. I., Yan M., Rosario D., Li L., de la Torre J. C., Zhang D. E. 2004; Role of ISG15 protease UBP43 (USP18) in innate immunity to viral infection. Nat Med 10:1374–1378 [View Article][PubMed]
    [Google Scholar]
  22. Sakurai-Yamashita Y., Shigematsu K., Yamashita K., Niwa M. 2006; Expression of MCP-1 in the hippocampus of SHRSP with ischemia-related delayed neuronal death. Cell Mol Neurobiol 26:821–829 [View Article][PubMed]
    [Google Scholar]
  23. Tumpey T. M., Szretter K. J., Van Hoeven N., Katz J. M., Kochs G., Haller O., García-Sastre A., Staeheli P. 2007; The Mx1 gene protects mice against the pandemic 1918 and highly lethal human H5N1 influenza viruses. J Virol 81:10818–10821 [View Article][PubMed]
    [Google Scholar]
  24. Warke R. V., Xhaja K., Martin K. J., Fournier M. F., Shaw S. K., Brizuela N., de Bosch N., Lapointe D., Ennis F. A.other authors 2003; Dengue virus induces novel changes in gene expression of human umbilical vein endothelial cells. J Virol 77:11822–11832 [View Article][PubMed]
    [Google Scholar]
  25. Warke R. V., Martin K. J., Giaya K., Shaw S. K., Rothman A. L., Bosch I. 2008; TRAIL is a novel antiviral protein against dengue virus. J Virol 82:555–564 [View Article][PubMed]
    [Google Scholar]
  26. Yang Y., Ye J., Yang X., Jiang R., Chen H., Cao S. 2011; Japanese encephalitis virus infection induces changes of mRNA profile of mouse spleen and brain. Virol J 8:80 [View Article][PubMed]
    [Google Scholar]
  27. Yuan W., Krug R. M. 2001; Influenza B virus NS1 protein inhibits conjugation of the interferon (IFN)-induced ubiquitin-like ISG15 protein. EMBO J 20:362–371 [View Article][PubMed]
    [Google Scholar]
  28. Zhao P., Zhao L., Zhang T., Qi Y., Wang T., Liu K., Wang H., Feng H., Jin H.other authors 2011; Innate immune response gene expression profiles in central nervous system of mice infected with rabies virus. Comp Immunol Microbiol Infect Dis 34:503–512 [View Article][PubMed]
    [Google Scholar]
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