1887

Abstract

Virions of the RPV strain of were purified from infected oat tissue and analysed by MS. Two conserved residues, K147 and K181, in the virus coat protein, were confidently identified to contain epsilon--acetyl groups. While no functional data are available for K147, K181 lies within an interfacial region critical for virion assembly and stability. The signature immonium ion at / 126.0919 demonstrated the presence of -acetyllysine, and the sequence fragment ions enabled an unambiguous assignment of the epsilon--acetyl modification on K181. We hypothesize that selection favours acetylation of K181 in a fraction of coat protein monomers to stabilize the capsid by promoting intermonomer salt bridge formation.

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2014-10-01
2021-07-26
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