1887

Abstract

The genus consists of insect-only viruses isolated from mosquitoes and sandflies. Here, we report the successful construction of a full-length infectious cDNA clone of Negev virus (NEGV) strain M30957. Viral RNA was transcribed and virus was readily rescued with or without the use of a cap analogue. These results strongly suggest that NEGV, and likely other members within the genus, is a non-segmented, single-stranded, positive-sense RNA virus.

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2014-09-01
2020-10-31
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References

  1. Ahlquist P., French R., Janda M., Loesch-Fries L. S. 1984; Multicomponent RNA plant virus infection derived from cloned viral cDNA. Proc Natl Acad Sci U S A 81:7066–7070 [CrossRef][PubMed]
    [Google Scholar]
  2. Dasmahapatra B., Dasgupta R., Saunders K., Selling B., Gallagher T., Kaesberg P. 1986; Infectious RNA derived by transcription from cloned cDNA copies of the genomic RNA of an insect virus. Proc Natl Acad Sci U S A 83:63–66 [CrossRef][PubMed]
    [Google Scholar]
  3. Liu Y., Wimmer E., Paul A. V. 2009; Cis-acting RNA elements in human and animal plus-strand RNA viruses. Biochim Biophys Acta 1789:495–517 [CrossRef][PubMed]
    [Google Scholar]
  4. Locali-Fabris E. C., Freitas-Astúa J., Souza A. A., Takita M. A., Astúa-Monge G., Antonioli-Luizon R., Rodrigues V., Targon M. L., Machado M. A. 2006; Complete nucleotide sequence, genomic organization and phylogenetic analysis of Citrus leprosis virus cytoplasmic type. J Gen Virol 87:2721–2729 [CrossRef][PubMed]
    [Google Scholar]
  5. Polo S., Ketner G., Levis R., Falgout B. 1997; Infectious RNA transcripts from full-length dengue virus type 2 cDNA clones made in yeast. J Virol 71:5366–5374[PubMed]
    [Google Scholar]
  6. Pugachev K. V., Abernathy E. S., Frey T. K. 1997; Improvement of the specific infectivity of the rubella virus (RUB) infectious clone: determinants of cytopathogenicity induced by RUB map to the nonstructural proteins. J Virol 71:562–568[PubMed]
    [Google Scholar]
  7. Rice C. M., Levis R., Strauss J. H., Huang H. V. 1987; Production of infectious RNA transcripts from Sindbis virus cDNA clones: mapping of lethal mutations, rescue of a temperature-sensitive marker, and in vitro mutagenesis to generate defined mutants. J Virol 61:3809–3819[PubMed]
    [Google Scholar]
  8. Sambrook J., Russell D. W. 2001 Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  9. Vasilakis N., Forrester N. L., Palacios G., Nasar F., Savji N., Rossi S. L., Guzman H., Wood T. G., Popov V.other authors 2013; Negevirus: a proposed new taxon of insect-specific viruses with wide geographic distribution. J Virol 87:2475–2488 [CrossRef][PubMed]
    [Google Scholar]
  10. Wimmer E., Paul A. V. 2011; Synthetic poliovirus and other designer viruses: what have we learned from them?. Annu Rev Microbiol 65:583–609 [CrossRef][PubMed]
    [Google Scholar]
  11. Wimmer E., Mueller S., Tumpey T. M., Taubenberger J. K. 2009; Synthetic viruses: a new opportunity to understand and prevent viral disease. Nat Biotechnol 27:1163–1172 [CrossRef][PubMed]
    [Google Scholar]
  12. Zuker M. 2003; Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406–3415 [CrossRef][PubMed]
    [Google Scholar]
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