RT Journal Article SR Electronic(1) A1 Sakaguchi, Shoichi A1 Nakagawa, So A1 Yoshikawa, Rokusuke A1 Kuwahara, Chieko A1 Hagiwara, Hiroko A1 Asai, Ken-ichi A1 Kawakami, Kazuo A1 Yamamoto, Yu A1 Ogawa, Makoto A1 Miyazawa, TakayukiYR 2014 T1 Genetic diversity of feline morbilliviruses isolated in Japan JF Journal of General Virology, VO 95 IS 7 SP 1464 OP 1468 DO https://doi.org/10.1099/vir.0.065029-0 PB Microbiology Society, SN 1465-2099, AB Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be associated with tubulointerstitial nephritis. Although FmoPV was first described in China in 2012, there has been no report of the isolation of this virus in other countries. In this report, we describe the isolation and characterization of FmoPV from domestic cats in Japan. By using reverse transcription (RT)-PCR, we found that three of 13 urine samples from cats brought to veterinary hospitals were positive for FmoPV. FmoPV strains SS1 to SS3 were isolated from the RT-PCR-positive urine samples. Crandell-Rees feline kidney (CRFK) cells exposed to FmoPV showed cytopathic effects with syncytia formation, and FmoPV N protein was detected by indirect immunofluorescence assays. In addition, pleomorphic virus particles with apparent glycoprotein envelope spikes were observed by electron microscopy. By sequence analysis of FmoPV H and L genes, we found that FmoPVs showed genetic diversity; however, signatures of positive selection were not identified., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.065029-0