1887

Journal of General Virology header logo

Volume 95, Issue 8

A newly identified protein complex that mediates white spot syndrome virus infection via chitin-binding protein Free

Abstract

White spot syndrome virus (WSSV) is a large enveloped virus which has caused severe mortality and huge economic losses in the shrimp farming industry. The enveloped virus must be combined with the receptors of the host cell membrane by the virus envelope proteins. In the case of WSSV, binding of envelope proteins with receptors of the host cell membrane was discovered in a number of previous studies, such as VP53A and 10 other proteins with chitin-binding protein (CBP), VP28 with Rab7, VP187 with β-integrin, and so on. WSSV envelope proteins were also considered capable of forming a protein complex dubbed an ‘infectome’. In this study, the research was focused on the role of CBP in the WSSV infection process, and the relationship between CBP and the envelope proteins VP24, VP28, VP31, VP32 VP39B, VP53A and VP56. The results of the reverse transcription-PCR analyses showed that CBP existed in a variety of shrimp. The speed of WSSV infection could be slowed down by inhibiting CBP gene expression. Far-Western blot analysis and His pull-down assays were conducted, and a protein complex was found that appeared to be composed of a ‘linker’ protein consisting of VP31, VP32 and VP39B together with four envelope proteins, including VP24, VP28, VP53A and VP56. This protein complex was possibly another part of the infectome and the possible binding region with CBP. The findings of this study may have identified certain points for further WSSV research.

  • Received:
  • Accepted:
  • Published Online:
© 2014 The Authors
Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.064782-0
2014-08-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/95/8/1799.html?itemId=/content/journal/jgv/10.1099/vir.0.064782-0&mimeType=html&fmt=ahah

References

  1. Chang Y. S., Liu W. J., Chou T. L., Lee Y. T., Lee T. L., Huang W. T., Kou G. H., Lo C. F. 2008; Characterization of white spot syndrome virus envelope protein VP51A and its interaction with viral tegument protein VP26. J Virol 82:12555–12564 [View Article][PubMed]
     [Google Scholar]
  2. Chang Y. S., Liu W. J., Lee C. C., Chou T. L., Lee Y. T., Wu T. S., Huang J. Y., Huang W. T., Lee T. L.other authors 2010; A 3D model of the membrane protein complex formed by the white spot syndrome virus structural proteins. PLoS ONE 5:e10718 [View Article][PubMed]
     [Google Scholar]
  3. Chen J., Li Z., Hew C. L. 2007a; Characterization of a novel envelope protein WSV010 of shrimp white spot syndrome virus and its interaction with a major viral structural protein VP24. Virology 364:208–213 [View Article][PubMed]
     [Google Scholar]
  4. Chen L. L., Lu L. C., Wu W. J., Lo C. F., Huang W. P. 2007b; White spot syndrome virus envelope protein VP53A interacts with Penaeus monodon chitin-binding protein (PmCBP). Dis Aquat Organ 74:171–178 [View Article][PubMed]
     [Google Scholar]
  5. Chen K. Y., Hsu T. C., Huang P. Y., Kang S. T., Lo C. F., Huang W. P., Chen L. L. 2009; Penaeus monodon chitin-binding protein (PmCBP) is involved in white spot syndrome virus (WSSV) infection. Fish Shellfish Immunol 27:460–465 [View Article][PubMed]
     [Google Scholar]
  6. Escobedo-Bonilla C. M., Alday-Sanz V., Wille M., Sorgeloos P., Pensaert M. B., Nauwynck H. J. 2008; A review on the morphology, molecular characterization, morphogenesis and pathogenesis of white spot syndrome virus. J Fish Dis 31:1–18 [View Article][PubMed]
     [Google Scholar]
  7. Feng Y., Broder C. C., Kennedy P. E., Berger E. A. 1996; HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science 272:872–877 [View Article][PubMed]
     [Google Scholar]
  8. Huang S., Endo R. I., Nemerow G. R. 1995; Upregulation of integrins alpha v beta 3 and alpha v beta 5 on human monocytes and T lymphocytes facilitates adenovirus-mediated gene delivery. J Virol 69:2257–2263[PubMed]
     [Google Scholar]
  9. Jie Z., Xu L., Yang F. 2008; The C-terminal region of envelope protein VP38 from white spot syndrome virus is indispensable for interaction with VP24. Arch Virol 153:2103–2106 [View Article][PubMed]
     [Google Scholar]
  10. Leu J. H., Yang F., Zhang X., Xu X., Kou G. H., Lo C. F. 2009; Whispovirus. Curr Top Microbiol Immunol 328:197–227[PubMed]
     [Google Scholar]
  11. Li L., Xie X., Yang F. 2005; Identification and characterization of a prawn white spot syndrome virus gene that encodes an envelope protein VP31. Virology 340:125–132 [View Article][PubMed]
     [Google Scholar]
  12. Li D. F., Zhang M. C., Yang H. J., Zhu Y. B., Xu X. 2007; β-Integrin mediates WSSV infection. Virology 368:122–132 [View Article][PubMed]
     [Google Scholar]
  13. Li L., Lin Z., Xu L., Yang F. 2011; The RGD motif in VP31 of white spot syndrome virus is involved in cell adhesion. Arch Virol 156:1317–1321 [View Article][PubMed]
     [Google Scholar]
  14. Liu Q. H., Ma C. Y., Chen W. B., Zhang X. L., Liang Y., Dong S. L., Huang J. 2009; White spot syndrome virus VP37 interacts with VP28 and VP26. Dis Aquat Organ 85:23–30 [View Article][PubMed]
     [Google Scholar]
  15. Lo C. F., Leu J. H., Ho C. H., Chen C. H., Peng S. E., Chen Y. T., Chou C. M., Yeh P. Y., Huang C. J.other authors 1996; Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction. Dis Aquat Organ 25:133–141 [View Article]
     [Google Scholar]
  16. Sritunyalucksana K., Wannapapho W., Lo C. F., Flegel T. W. 2006; PmRab7 is a VP28-binding protein involved in white spot syndrome virus infection in shrimp. J Virol 80:10734–10742 [View Article][PubMed]
     [Google Scholar]
  17. Tsai J. M., Wang H. C., Leu J. H., Hsiao H. H., Wang A. H. J., Kou G. H., Lo C. F. 2004; Genomic and proteomic analysis of thirty-nine structural proteins of shrimp white spot syndrome virus. J Virol 78:11360–11370 [View Article][PubMed]
     [Google Scholar]
  18. Tsai J. M., Wang H. C., Leu J. H., Wang A. H. J., Zhuang Y., Walker P. J., Kou G. H., Lo C. F. 2006; Identification of the nucleocapsid, tegument, and envelope proteins of the shrimp white spot syndrome virus virion. J Virol 80:3021–3029 [View Article][PubMed]
     [Google Scholar]
  19. Wan Q., Xu L., Yang F. 2008; VP26 of white spot syndrome virus functions as a linker protein between the envelope and nucleocapsid of virions by binding with VP51. J Virol 82:12598–12601 [View Article][PubMed]
     [Google Scholar]
  20. Wang C. H., Lo C. F., Leu J. H., Chou C. M., Yeh P. Y., Chou H. Y., Tung M. C., Chang C. F., Su M. S., Kou G. H. 1995; Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon . Dis Aquat Organ 23:239–242 [View Article]
     [Google Scholar]
  21. Wang K. C., Kondo H., Hirono I., Aoki T. 2010; The Marsupenaeus japonicus voltage-dependent anion channel (MjVDAC) protein is involved in white spot syndrome virus (WSSV) pathogenesis. Fish Shellfish Immunol 29:94–103 [View Article][PubMed]
     [Google Scholar]
  22. Witteveldt J., Cifuentes C. C., Vlak J. M., van Hulten M. C. 2004; Protection of Penaeus monodon against white spot syndrome virus by oral vaccination. J Virol 78:2057–2061 [View Article][PubMed]
     [Google Scholar]
  23. Wongteerasupaya C., Vickers J. E., Sriurairatana S., Nash G. L., Akarajamorn A., Boonsaeng V., Panyim S., Tassanakajon A., Withyanchumnarnkul B., Flegel T. W. 1995; A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in black tiger prawn Penaeus monodon . Dis Aquat Organ 21:69–77 [View Article]
     [Google Scholar]
  24. Xie X., Yang F. 2006; White spot syndrome virus VP24 interacts with VP28 and is involved in virus infection. J Gen Virol 87:1903–1908 [View Article][PubMed]
     [Google Scholar]
  25. Xie X., Li H., Xu L., Yang F. 2005; A simple and efficient method for purification of intact white spot syndrome virus (WSSV) viral particles. Virus Res 108:63–67 [View Article][PubMed]
     [Google Scholar]
  26. Xie X., Xu L., Yang F. 2006; Proteomic analysis of the major envelope and nucleocapsid proteins of white spot syndrome virus. J Virol 80:10615–10623 [View Article][PubMed]
     [Google Scholar]
  27. Zhang X., Huang C., Tang X., Zhuang Y., Hew C. L. 2004; Identification of structural proteins from shrimp white spot syndrome virus (WSSV) by 2DE-MS. Proteins 55:229–235 [View Article][PubMed]
     [Google Scholar]
  28. Zhou Q., Qi Y. P., Yang F. 2007; Application of spectrophotometry to evaluate the concentration of purified White Spot Syndrome Virus. J Virol Methods 146:288–292 [View Article][PubMed]
     [Google Scholar]
  29. Zhou Q., Xu L., Li H., Qi Y. P., Yang F. 2009; Four major envelope proteins of white spot syndrome virus bind to form a complex. J Virol 83:4709–4712 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.064782-0
Loading
A newly identified protein complex that mediates white spot syndrome virus infection via chitin-binding protein
J. Gen. Virol. 95, 1799 (2014); https://doi.org/10.1099/vir.0.064782-0
/content/journal/jgv/10.1099/vir.0.064782-0
/content/journal/jgv/10.1099/vir.0.064782-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error