1887

Abstract

We identified breast cancer-associated protein (BCA3) as a novel binding partner of Mason-Pfizer monkey virus (MPMV) protease (PR). The interaction was confirmed by co-immunoprecipitation and immunocolocalization of MPMV PR and BCA3. Full-length but not C-terminally truncated BCA3 was incorporated into MPMV virions. We ruled out the potential role of the G-patch domain, a glycine-rich domain located at the C terminus of MPMV PR, in BCA3 interaction and virion incorporation. Expression of BCA3 did not affect MPMV particle release and proteolytic processing; however, it slightly increased MPMV infectivity.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.064345-0
2014-06-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/95/6/1383.html?itemId=/content/journal/jgv/10.1099/vir.0.064345-0&mimeType=html&fmt=ahah

References

  1. Brody B. A., Hunter E.. ( 1992; ). Mutations within the env gene of Mason-Pfizer monkey virus: effects on protein transport and SU-TM association. . J Virol 66:, 3466–3475.[PubMed]
    [Google Scholar]
  2. Burger A., Li H., Zhang X. K., Pienkowska M., Venanzoni M., Vournakis J., Papas T., Seth A.. ( 1998; ). Breast cancer genome anatomy: correlation of morphological changes in breast carcinomas with expression of the novel gene product Di12. . Oncogene 16:, 327–333. [CrossRef] [PubMed]
    [Google Scholar]
  3. Gao F., Cheng J., Shi T., Yeh E. T. H.. ( 2006; ). Neddylation of a breast cancer-associated protein recruits a class III histone deacetylase that represses NFkappaB-dependent transcription. . Nat Cell Biol 8:, 1171–1177. [CrossRef] [PubMed]
    [Google Scholar]
  4. Gao N., Asamitsu K., Hibi Y., Ueno T., Okamoto T.. ( 2008; ). AKIP1 enhances NF-kappaB-dependent gene expression by promoting the nuclear retention and phosphorylation of p65. . J Biol Chem 283:, 7834–7843. [CrossRef] [PubMed]
    [Google Scholar]
  5. Gao N., Hibi Y., Cueno M., Asamitsu K., Okamoto T.. ( 2010; ). A-kinase-interacting protein 1 (AKIP1) acts as a molecular determinant of PKA in NF-kappaB signaling. . J Biol Chem 285:, 28097–28104. [CrossRef] [PubMed]
    [Google Scholar]
  6. King C. C., Sastri M., Chang P., Pennypacker J., Taylor S. S.. ( 2011; ). The rate of NF-κB nuclear translocation is regulated by PKA and A kinase interacting protein 1. . PLoS ONE 6:, e18713. [CrossRef] [PubMed]
    [Google Scholar]
  7. Kitching R., Li H., Wong M. J., Kanaganayakam S., Kahn H., Seth A.. ( 2003; ). Characterization of a novel human breast cancer associated gene (BCA3) encoding an alternatively spliced proline-rich protein. . Biochim Biophys Acta 1625:, 116–121. [CrossRef] [PubMed]
    [Google Scholar]
  8. Křízová I., Hadravová R., Štokrová J., Günterová J., Doležal M., Ruml T., Rumlová M., Pichová I.. ( 2012; ). The G-patch domain of Mason-Pfizer monkey virus is a part of reverse transcriptase. . J Virol 86:, 1988–1998. [CrossRef] [PubMed]
    [Google Scholar]
  9. Leung T. H.-Y., Ngan H. Y.-S.. ( 2010; ). Interaction of TAp73 and breast cancer-associated gene 3 enhances the sensitivity of cervical cancer cells in response to irradiation-induced apoptosis. . Cancer Res 70:, 6486–6496. [CrossRef] [PubMed]
    [Google Scholar]
  10. Newman R. M., Hall L., Connole M., Chen G. L., Sato S., Yuste E., Diehl W., Hunter E., Kaur A.. & other authors ( 2006; ). Balancing selection and the evolution of functional polymorphism in Old World monkey TRIM5alpha. . Proc Natl Acad Sci U S A 103:, 19134–19139. [CrossRef] [PubMed]
    [Google Scholar]
  11. Sastri M., Barraclough D. M., Carmichael P. T., Taylor S. S.. ( 2005; ). A-kinase-interacting protein localizes protein kinase A in the nucleus. . Proc Natl Acad Sci U S A 102:, 349–354. [CrossRef] [PubMed]
    [Google Scholar]
  12. Sastri M., Haushalter K. J., Panneerselvam M., Chang P., Fridolfsson H., Finley J. C., Ng D., Schilling J. M., Miyanohara A.. & other authors ( 2013; ). A kinase interacting protein (AKIP1) is a key regulator of cardiac stress. . Proc Natl Acad Sci U S A 110:, E387–E396. [CrossRef] [PubMed]
    [Google Scholar]
  13. Svec M., Bauerová H., Pichová I., Konvalinka J., Strísovský K.. ( 2004; ). Proteinases of betaretroviruses bind single-stranded nucleic acids through a novel interaction module, the G-patch. . FEBS Lett 576:, 271–276. [CrossRef] [PubMed]
    [Google Scholar]
  14. Zábranská H., Tůma R., Kluh I., Svatos A., Ruml T., Hrabal R., Pichová I.. ( 2007; ). The role of the S-S bridge in retroviral protease function and virion maturation. . J Mol Biol 365:, 1493–1504. [CrossRef] [PubMed]
    [Google Scholar]
  15. Zábranský A., Andreánsky M., Hrusková-Heidingsfeldová O., Havlícek V., Hunter E., Ruml T., Pichová I.. ( 1998; ). Three active forms of aspartic proteinase from Mason-Pfizer monkey virus. . Virology 245:, 250–256. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.064345-0
Loading
/content/journal/jgv/10.1099/vir.0.064345-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error