1887

Abstract

Difficulties associated with efficient delivery and targeting of miRNAs to cells is hampering the real world application of miRNA technology. This study utilized an influenza A-based delivery system to express miR-155 in order to knockdown mRNA. Using qPCR and dual luciferase technology we show that miR-155 delivery resulted in a significant increase in cellular miR-155 which facilitated a downregulation of gene expression and a functional increase in IL-6 and IFN-β cytokines.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.063834-0
2014-09-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/95/9/1880.html?itemId=/content/journal/jgv/10.1099/vir.0.063834-0&mimeType=html&fmt=ahah

References

  1. Baltimore D., Boldin M. P., O’Connell R. M., Rao D. S., Taganov K. D. 2008; MicroRNAs: new regulators of immune cell development and function. Nat Immunol 9:839–845 [View Article][PubMed]
    [Google Scholar]
  2. Fenner J. E., Starr R., Cornish A. L., Zhang J. G., Metcalf D., Schreiber R. D., Sheehan K., Hilton D. J., Alexander W. S., Hertzog P. J. 2006; Suppressor of cytokine signaling 1 regulates the immune response to infection by a unique inhibition of type I interferon activity. Nat Immunol 7:33–39 [View Article][PubMed]
    [Google Scholar]
  3. Goodwin K., Viboud C., Simonsen L. 2006; Antibody response to influenza vaccination in the elderly: a quantitative review. Vaccine 24:1159–1169 [View Article][PubMed]
    [Google Scholar]
  4. Hoffmann E., Neumann G., Kawaoka Y., Hobom G., Webster R. G. 2000; A DNA transfection system for generation of influenza A virus from eight plasmids. Proc Natl Acad Sci U S A 97:6108–6113 [View Article][PubMed]
    [Google Scholar]
  5. Hoffmann E., Stech J., Guan Y., Webster R. G., Perez D. R. 2001; Universal primer set for the full-length amplification of all influenza A viruses. Arch Virol 146:2275–2289 [View Article][PubMed]
    [Google Scholar]
  6. Lamb R. A., Lai C. J., Choppin P. W. 1981; Sequences of mRNAs derived from genome RNA segment 7 of influenza virus: colinear and interrupted mRNAs code for overlapping proteins. Proc Natl Acad Sci U S A 78:4170–4174 [View Article][PubMed]
    [Google Scholar]
  7. Langlois R. A., Albrecht R. A., Kimble B., Sutton T., Shapiro J. S., Finch C., Angel M., Chua M. A., Gonzalez-Reiche A. S.other authors 2013; MicroRNA-based strategy to mitigate the risk of gain-of-function influenza studies. Nat Biotechnol 31:844–847 [View Article][PubMed]
    [Google Scholar]
  8. Lindsay M. A. 2008; microRNAs and the immune response. Trends Immunol 29:343–351 [View Article][PubMed]
    [Google Scholar]
  9. Lu L. F., Thai T. H., Calado D. P., Chaudhry A., Kubo M., Tanaka K., Loeb G. B., Lee H., Yoshimura A.other authors 2009; Foxp3-dependent microRNA155 confers competitive fitness to regulatory T cells by targeting SOCS1 protein. Immunity 30:80–91 [View Article][PubMed]
    [Google Scholar]
  10. O’Connell R. M., Rao D. S., Chaudhuri A. A., Boldin M. P., Taganov K. D., Nicoll J., Paquette R. L., Baltimore D. 2008; Sustained expression of microRNA-155 in hematopoietic stem cells causes a myeloproliferative disorder. J Exp Med 205:585–594 [View Article][PubMed]
    [Google Scholar]
  11. Olsen P. H., Ambros V. 1999; The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation. Dev Biol 216:671–680 [View Article][PubMed]
    [Google Scholar]
  12. Perez J. T., Pham A. M., Lorini M. H., Chua M. A., Steel J., tenOever B. R. 2009; MicroRNA-mediated species-specific attenuation of influenza A virus. Nat Biotechnol 27:572–576 [View Article][PubMed]
    [Google Scholar]
  13. Pothlichet J., Chignard M., Si-Tahar M. 2008; Cutting edge: innate immune response triggered by influenza A virus is negatively regulated by SOCS1 and SOCS3 through a RIG-I/IFNAR1-dependent pathway. J Immunol 180:2034–2038 [View Article][PubMed]
    [Google Scholar]
  14. Schmid S., Zony L. C., tenOever B. R. 2014; A versatile RNA vector for delivery of coding and noncoding RNAs. J Virol 88:2333–2336 [View Article][PubMed]
    [Google Scholar]
  15. Shi R., Chiang V. L. 2005; Facile means for quantifying microRNA expression by real-time PCR. Biotechniques 39:519–525 [View Article][PubMed]
    [Google Scholar]
  16. Stegmeier F., Hu G., Rickles R. J., Hannon G. J., Elledge S. J. 2005; A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Proc Natl Acad Sci U S A 102:13212–13217 [View Article][PubMed]
    [Google Scholar]
  17. Sun Y., Cai J., Ma F., P., Huang H., Zhou J. 2012; miR-155 mediates suppressive effect of progesterone on TLR3, TLR4-triggered immune response. Immunol Lett 146:25–30 [View Article][PubMed]
    [Google Scholar]
  18. Tiscornia G., Tergaonkar V., Galimi F., Verma I. M. 2004; CRE recombinase-inducible RNA interference mediated by lentiviral vectors. Proc Natl Acad Sci U S A 101:7347–7351 [View Article][PubMed]
    [Google Scholar]
  19. Varble A., ten Oever B. R. 2011; Implications of RNA virus-produced miRNAs. RNA Biol 8:190–194 [View Article][PubMed]
    [Google Scholar]
  20. Varble A., Chua M. A., Perez J. T., Manicassamy B., García-Sastre A., tenOever B. R. 2010; Engineered RNA viral synthesis of microRNAs. Proc Natl Acad Sci U S A 107:11519–11524 [View Article][PubMed]
    [Google Scholar]
  21. Wang P., Hou J., Lin L., Wang C., Liu X., Li D., Ma F., Wang Z., Cao X. 2010; Inducible microRNA-155 feedback promotes type I IFN signaling in antiviral innate immunity by targeting suppressor of cytokine signaling 1. J Immunol 185:6226–6233 [View Article][PubMed]
    [Google Scholar]
  22. Zeng Y., Yi R., Cullen B. R. 2003; MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms. Proc Natl Acad Sci U S A 100:9779–9784 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.063834-0
Loading
/content/journal/jgv/10.1099/vir.0.063834-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error