1887

Abstract

Difficulties associated with efficient delivery and targeting of miRNAs to cells is hampering the real world application of miRNA technology. This study utilized an influenza A-based delivery system to express miR-155 in order to knockdown mRNA. Using qPCR and dual luciferase technology we show that miR-155 delivery resulted in a significant increase in cellular miR-155 which facilitated a downregulation of gene expression and a functional increase in IL-6 and IFN-β cytokines.

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2014-09-01
2019-11-17
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References

  1. Baltimore D., Boldin M. P., O’Connell R. M., Rao D. S., Taganov K. D.. ( 2008;). MicroRNAs: new regulators of immune cell development and function. . Nat Immunol 9:, 839–845. [CrossRef][PubMed]
    [Google Scholar]
  2. Fenner J. E., Starr R., Cornish A. L., Zhang J. G., Metcalf D., Schreiber R. D., Sheehan K., Hilton D. J., Alexander W. S., Hertzog P. J.. ( 2006;). Suppressor of cytokine signaling 1 regulates the immune response to infection by a unique inhibition of type I interferon activity. . Nat Immunol 7:, 33–39. [CrossRef][PubMed]
    [Google Scholar]
  3. Goodwin K., Viboud C., Simonsen L.. ( 2006;). Antibody response to influenza vaccination in the elderly: a quantitative review. . Vaccine 24:, 1159–1169. [CrossRef][PubMed]
    [Google Scholar]
  4. Hoffmann E., Neumann G., Kawaoka Y., Hobom G., Webster R. G.. ( 2000;). A DNA transfection system for generation of influenza A virus from eight plasmids. . Proc Natl Acad Sci U S A 97:, 6108–6113. [CrossRef][PubMed]
    [Google Scholar]
  5. Hoffmann E., Stech J., Guan Y., Webster R. G., Perez D. R.. ( 2001;). Universal primer set for the full-length amplification of all influenza A viruses. . Arch Virol 146:, 2275–2289. [CrossRef][PubMed]
    [Google Scholar]
  6. Lamb R. A., Lai C. J., Choppin P. W.. ( 1981;). Sequences of mRNAs derived from genome RNA segment 7 of influenza virus: colinear and interrupted mRNAs code for overlapping proteins. . Proc Natl Acad Sci U S A 78:, 4170–4174. [CrossRef][PubMed]
    [Google Scholar]
  7. Langlois R. A., Albrecht R. A., Kimble B., Sutton T., Shapiro J. S., Finch C., Angel M., Chua M. A., Gonzalez-Reiche A. S.. & other authors ( 2013;). MicroRNA-based strategy to mitigate the risk of gain-of-function influenza studies. . Nat Biotechnol 31:, 844–847. [CrossRef][PubMed]
    [Google Scholar]
  8. Lindsay M. A.. ( 2008;). microRNAs and the immune response. . Trends Immunol 29:, 343–351. [CrossRef][PubMed]
    [Google Scholar]
  9. Lu L. F., Thai T. H., Calado D. P., Chaudhry A., Kubo M., Tanaka K., Loeb G. B., Lee H., Yoshimura A.. & other authors ( 2009;). Foxp3-dependent microRNA155 confers competitive fitness to regulatory T cells by targeting SOCS1 protein. . Immunity 30:, 80–91. [CrossRef][PubMed]
    [Google Scholar]
  10. O’Connell R. M., Rao D. S., Chaudhuri A. A., Boldin M. P., Taganov K. D., Nicoll J., Paquette R. L., Baltimore D.. ( 2008;). Sustained expression of microRNA-155 in hematopoietic stem cells causes a myeloproliferative disorder. . J Exp Med 205:, 585–594. [CrossRef][PubMed]
    [Google Scholar]
  11. Olsen P. H., Ambros V.. ( 1999;). The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation. . Dev Biol 216:, 671–680. [CrossRef][PubMed]
    [Google Scholar]
  12. Perez J. T., Pham A. M., Lorini M. H., Chua M. A., Steel J., tenOever B. R.. ( 2009;). MicroRNA-mediated species-specific attenuation of influenza A virus. . Nat Biotechnol 27:, 572–576. [CrossRef][PubMed]
    [Google Scholar]
  13. Pothlichet J., Chignard M., Si-Tahar M.. ( 2008;). Cutting edge: innate immune response triggered by influenza A virus is negatively regulated by SOCS1 and SOCS3 through a RIG-I/IFNAR1-dependent pathway. . J Immunol 180:, 2034–2038. [CrossRef][PubMed]
    [Google Scholar]
  14. Schmid S., Zony L. C., tenOever B. R.. ( 2014;). A versatile RNA vector for delivery of coding and noncoding RNAs. . J Virol 88:, 2333–2336. [CrossRef][PubMed]
    [Google Scholar]
  15. Shi R., Chiang V. L.. ( 2005;). Facile means for quantifying microRNA expression by real-time PCR. . Biotechniques 39:, 519–525. [CrossRef][PubMed]
    [Google Scholar]
  16. Stegmeier F., Hu G., Rickles R. J., Hannon G. J., Elledge S. J.. ( 2005;). A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. . Proc Natl Acad Sci U S A 102:, 13212–13217. [CrossRef][PubMed]
    [Google Scholar]
  17. Sun Y., Cai J., Ma F., P., Huang H., Zhou J.. ( 2012;). miR-155 mediates suppressive effect of progesterone on TLR3, TLR4-triggered immune response. . Immunol Lett 146:, 25–30. [CrossRef][PubMed]
    [Google Scholar]
  18. Tiscornia G., Tergaonkar V., Galimi F., Verma I. M.. ( 2004;). CRE recombinase-inducible RNA interference mediated by lentiviral vectors. . Proc Natl Acad Sci U S A 101:, 7347–7351. [CrossRef][PubMed]
    [Google Scholar]
  19. Varble A., ten Oever B. R.. ( 2011;). Implications of RNA virus-produced miRNAs. . RNA Biol 8:, 190–194. [CrossRef][PubMed]
    [Google Scholar]
  20. Varble A., Chua M. A., Perez J. T., Manicassamy B., García-Sastre A., tenOever B. R.. ( 2010;). Engineered RNA viral synthesis of microRNAs. . Proc Natl Acad Sci U S A 107:, 11519–11524. [CrossRef][PubMed]
    [Google Scholar]
  21. Wang P., Hou J., Lin L., Wang C., Liu X., Li D., Ma F., Wang Z., Cao X.. ( 2010;). Inducible microRNA-155 feedback promotes type I IFN signaling in antiviral innate immunity by targeting suppressor of cytokine signaling 1. . J Immunol 185:, 6226–6233. [CrossRef][PubMed]
    [Google Scholar]
  22. Zeng Y., Yi R., Cullen B. R.. ( 2003;). MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms. . Proc Natl Acad Sci U S A 100:, 9779–9784. [CrossRef][PubMed]
    [Google Scholar]
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