1887

Abstract

Infections with bovine viral diarrhea virus (BVDV) have a huge economic impact on cattle production and reproduction worldwide. A key factor for BVDV surveillance and eventual eradication is to efficiently detect infections and to monitor herd immunity. In this study, we generated a stable, bi-cistronic BVDV that encoded EGFP in addition to the viral proteins. Applying this recombinant virus, a new flow-cytometry-based virus neutralization test was established that enabled accurate and reliable detection of field-virus-infected and vaccinated animals. The test, which is simple and fast, is expected to support novel, effective screening procedures in eradication and vaccination programmes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.063800-0
2014-07-01
2021-02-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/95/7/1522.html?itemId=/content/journal/jgv/10.1099/vir.0.063800-0&mimeType=html&fmt=ahah

References

  1. Austermann-Busch S., Becher P. 2012; RNA structural elements determine frequency and sites of nonhomologous recombination in an animal plus-strand RNA virus. J Virol 86:7393–7402 [CrossRef][PubMed]
    [Google Scholar]
  2. Avalos-Ramirez R., Orlich M., Thiel H. J., Becher P. 2001; Evidence for the presence of two novel pestivirus species. Virology 286:456–465 [CrossRef][PubMed]
    [Google Scholar]
  3. Baker J. C. 1990; Clinical aspects of bovine virus diarrhoea virus infection. Rev Sci Tech 9:25–41[PubMed]
    [Google Scholar]
  4. Baroth M., Peters Y., Schönbrunner E. R., Behrens S. E. 2010; Stable recombinants of bovine viral diarrhea virus containing a hepatitis C virus insert. J Gen Virol 91:1213–1217 [CrossRef][PubMed]
    [Google Scholar]
  5. Becher P., Orlich M., Thiel H. J. 1998; Complete genomic sequence of border disease virus, a pestivirus from sheep. J Virol 72:5165–5173[PubMed]
    [Google Scholar]
  6. Behrens S. E., Isken O. 2006; Cis- and trans-acting determinants of Flaviviridae replication. In Molecular Biology of the Flavivirus pp. 101–134 Edited by Kalitzky M., Borowski P. Wymondham: Horizon Biosciences;
    [Google Scholar]
  7. Behrens S. E., Grassmann C. W., Thiel H. J., Meyers G., Tautz N. 1998; Characterization of an autonomous subgenomic pestivirus RNA replicon. J Virol 72:2364–2372[PubMed]
    [Google Scholar]
  8. Black C. B., Duensing T. D., Trinkle L. S., Dunlay R. T. 2011; Cell-based screening using high-throughput flow cytometry. Assay Drug Dev Technol 9:13–20 [CrossRef][PubMed]
    [Google Scholar]
  9. Bruschke C. J., Moormann R. J., van Oirschot J. T., van Rijn P. A. 1997; A subunit vaccine based on glycoprotein E2 of bovine virus diarrhea virus induces fetal protection in sheep against homologous challenge. Vaccine 15:1940–1945 [CrossRef][PubMed]
    [Google Scholar]
  10. Corapi W. V., Donis R. O., Dubovi E. J. 1990; Characterization of a panel of monoclonal antibodies and their use in the study of the antigenic diversity of bovine viral diarrhea virus. Am J Vet Res 51:1388–1394[PubMed]
    [Google Scholar]
  11. Cosma A., Bühler S., Nagaraj R., Staib C., Hammarin A. L., Wahren B., Goebel F. D., Erfle V., Sutter G. 2004; Neutralization assay using a modified vaccinia virus Ankara vector expressing the green fluorescent protein is a high-throughput method to monitor the humoral immune response against vaccinia virus. Clin Diagn Lab Immunol 11:406–410[PubMed]
    [Google Scholar]
  12. Couvreur B., Letellier C., Collard A., Quenon P., Dehan P., Hamers C., Pastoret P. P., Kerkhofs P. 2002; Genetic and antigenic variability in bovine viral diarrhea virus (BVDV) isolates from Belgium. Virus Res 85:17–28 [CrossRef][PubMed]
    [Google Scholar]
  13. Deng R., Brock K. V. 1993; 5′ and 3′ untranslated regions of pestivirus genome: primary and secondary structure analyses. Nucleic Acids Res 21:1949–1957 [CrossRef][PubMed]
    [Google Scholar]
  14. Dubovi E. J. 2013; Laboratory diagnosis of bovine viral diarrhea virus. Biologicals 41:8–13 [CrossRef][PubMed]
    [Google Scholar]
  15. El Omari K., Iourin O., Harlos K., Grimes J. M., Stuart D. I. 2013; Structure of a pestivirus envelope glycoprotein E2 clarifies its role in cell entry. Cell Reports 3:30–35 [CrossRef][PubMed]
    [Google Scholar]
  16. Fan Z. C., Dennis J. C., Bird R. C. 2008; Bovine viral diarrhea virus is a suitable viral vector for stable expression of heterologous gene when inserted in between Npro and C genes. Virus Res 138:97–104 [CrossRef][PubMed]
    [Google Scholar]
  17. Frey H. R., Eicken K., Grummer B., Kenklies S., Oguzoglu T. C., Moennig V. 2002; Foetal protection against bovine virus diarrhoea virus after two-step vaccination. J Vet Med B Infect Dis Vet Public Health 49:489–493 [CrossRef][PubMed]
    [Google Scholar]
  18. Houe H., Lindberg A., Moennig V. 2006; Test strategies in bovine viral diarrhea virus control and eradication campaigns in Europe. J Vet Diagn Invest 18:427–436 [CrossRef][PubMed]
    [Google Scholar]
  19. Isken O., Grassmann C. W., Sarisky R. T., Kann M., Zhang S., Grosse F., Kao P. N., Behrens S. E. 2003; Members of the NF90/NFAR protein group are involved in the life cycle of a positive-strand RNA virus. EMBO J 22:5655–5665 [CrossRef][PubMed]
    [Google Scholar]
  20. Isken O., Grassmann C. W., Yu H., Behrens S. E. 2004; Complex signals in the genomic 3′ nontranslated region of bovine viral diarrhea virus coordinate translation and replication of the viral RNA. RNA 10:1637–1652 [CrossRef][PubMed]
    [Google Scholar]
  21. Kim M. J., Kao C. 2001; Factors regulating template switch in vitro by viral RNA-dependent RNA polymerases: implications for RNA–RNA recombination. Proc Natl Acad Sci U S A 98:4972–4977 [CrossRef][PubMed]
    [Google Scholar]
  22. Lindberg A., Houe H. 2005; Characteristics in the epidemiology of bovine viral diarrhea virus (BVDV) of relevance to control. Prev Vet Med 72:55–73 discussion 215–219 [CrossRef][PubMed]
    [Google Scholar]
  23. Lindenbach B. D., Thiel H. J., Rice C. M. 2007; Flaviviridae: the viruses and their replication. In Fields Virology, 5th edn. pp. 1101–1151 Edited by Knipe D. M., Howley P. M., Griffin D. E., Lamb R. A., Martin M. A., Roizman B., Straus S. E. Philadelphia, PA: Lippincott Williams & Wilkins;
    [Google Scholar]
  24. Mendez E., Ruggli N., Collett M. S., Rice C. M. 1998; Infectious bovine viral diarrhea virus (strain NADL) RNA from stable cDNA clones: a cellular insert determines NS3 production and viral cytopathogenicity. J Virol 72:4737–4745[PubMed]
    [Google Scholar]
  25. Meyer C., Von Freyburg M., Elbers K., Meyers G. 2002; Recovery of virulent and RNase-negative attenuated type 2 bovine viral diarrhea viruses from infectious cDNA clones. J Virol 76:8494–8503 [CrossRef][PubMed]
    [Google Scholar]
  26. Meyers G., Tautz N., Becher P., Thiel H. J., Kümmerer B. M. 1996; Recovery of cytopathogenic and noncytopathogenic bovine viral diarrhea viruses from cDNA constructs. J Virol 70:8606–8613[PubMed]
    [Google Scholar]
  27. Mischkale K., Reimann I., Zemke J., König P., Beer M. 2010; Characterisation of a new infectious full-length cDNA clone of BVDV genotype 2 and generation of virus mutants. Vet Microbiol 142:3–12 [CrossRef][PubMed]
    [Google Scholar]
  28. Moennig V., Houe H., Lindberg A. 2005; BVD control in Europe: current status and perspectives. Anim Health Res Rev 6:63–74 [CrossRef][PubMed]
    [Google Scholar]
  29. Nagy P. D., Pogany J., Simon A. E. 1999; RNA elements required for RNA recombination function as replication enhancers in vitro and in vivo in a plus-strand RNA virus. EMBO J 18:5653–5665 [CrossRef][PubMed]
    [Google Scholar]
  30. Niskanen R. 1993; Relationship between the levels of antibodies to bovine viral diarrhoea virus in bulk tank milk and the prevalence of cows exposed to the virus. Vet Rec 133:341–344 [CrossRef][PubMed]
    [Google Scholar]
  31. Pankraz A., Thiel H. J., Becher P. 2005; Essential and nonessential elements in the 3′ nontranslated region of Bovine viral diarrhea virus. J Virol 79:9119–9127 [CrossRef][PubMed]
    [Google Scholar]
  32. Ridpath J. 2012; Preventive strategy for BVDV infection in North America. Jpn J Vet Res 60:SupplS41–S49[PubMed]
    [Google Scholar]
  33. Ridpath J. F. 2013; Immunology of BVDV vaccines. Biologicals 41:14–19 [CrossRef][PubMed]
    [Google Scholar]
  34. Sandvik T. 2005; Selection and use of laboratory diagnostic assays in BVD control programmes. Prev Vet Med 72:3–16 discussion 215–219 [CrossRef][PubMed]
    [Google Scholar]
  35. Ståhl K., Alenius S. 2012; BVDV control and eradication in Europe – an update. Jpn J Vet Res 60:SupplS31–S39[PubMed]
    [Google Scholar]
  36. Tautz N., Meyers G., Thiel H.-J. 1998; Pathogenesis of mucosal disease, a deadly disease of cattle caused by a pestivirus. Clin Diagn Virol 10:121–127 [CrossRef][PubMed]
    [Google Scholar]
  37. van Rijn P. A. 2007; A common neutralizing epitope on envelope glycoprotein E2 of different pestiviruses: implications for improvement of vaccines and diagnostics for classical swine fever (CSF)?. Vet Microbiol 125:150–156 [CrossRef][PubMed]
    [Google Scholar]
  38. Vassilev V. B., Collett M. S., Donis R. O. 1997; Authentic and chimeric full-length genomic cDNA clones of bovine viral diarrhea virus that yield infectious transcripts. J Virol 71:471–478[PubMed]
    [Google Scholar]
  39. Wang Z., Nie Y., Wang P., Ding M., Deng H. 2004; Characterization of classical swine fever virus entry by using pseudotyped viruses: E1 and E2 are sufficient to mediate viral entry. Virology 330:332–341 [CrossRef][PubMed]
    [Google Scholar]
  40. Yu H., Grassmann C. W., Behrens S. E. 1999; Sequence and structural elements at the 3′ terminus of bovine viral diarrhea virus genomic RNA: functional role during RNA replication. J Virol 73:3638–3648[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.063800-0
Loading
/content/journal/jgv/10.1099/vir.0.063800-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error