1887

Abstract

A novel reovirus, designated Cimodo virus (CMDV), was isolated from mosquitoes collected in a rainforest region in Côte d’Ivoire. The entire genome comprised 24 835 bp divided into 12 segments ranging from 585 to 4080 bp. The icosahedral non-enveloped virions were 80 nm in diameter. Eight major viral proteins of about 150, 135, 120, 80, 66, 59, 42 and 30 kDa were identified and seven proteins were mapped to the corresponding genome segments by liquid chromatography mass spectrometry. Predicted protein genes diverged by >77 % encoded amino acids from their closest reovirus relatives. The deep phylogenetic branching suggests that CMDV defines an as-yet-unidentified genus within the subfamily .

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2014-04-01
2019-11-17
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References

  1. Attoui H., Mohd Jaafar F., de Micco P., de Lamballerie X.. ( 2005; ). Coltiviruses and seadornaviruses in North America, Europe, and Asia. . Emerg Infect Dis 11:, 1673–1679. [CrossRef] [PubMed]
    [Google Scholar]
  2. Attoui H., Mertens P. P. C., Becnel J., Belaganahalli S., Bergoin M., Brussaard C. P., Chappell J. D., Ciarlet M., del Vas M.. & other authors ( 2012; ). Family Reoviridae. Amsterdam:: Elsevier/Academic Press;.
    [Google Scholar]
  3. Charrel R. N., Attoui H., Butenko A. M., Clegg J. C., Deubel V., Frolova T. V., Gould E. A., Gritsun T. S., Heinz F. X.. & other authors ( 2004; ). Tick-borne virus diseases of human interest in Europe. . Clin Microbiol Infect 10:, 1040–1055. [CrossRef] [PubMed]
    [Google Scholar]
  4. Chastel C., Main A. J., Couatarmanac’h A., Le Lay G., Knudson D. L., Quillien M. C., Beaucournu J. C.. ( 1984; ). Isolation of Eyach virus (Reoviridae, Colorado tick fever group) from Ixodes ricinus and I. ventalloi ticks in France. . Arch Virol 82:, 161–171. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chen J., Xiong J., Yang J., Mao Z., Chen X.. ( 2011; ). Nucleotide sequences of four RNA segments of a reovirus isolated from the mud crab Scylla serrata provide evidence that this virus belongs to a new genus in the family Reoviridae . . Arch Virol 156:, 523–528. [CrossRef] [PubMed]
    [Google Scholar]
  6. Corman V. M., Kallies R., Philipps H., Göpner G., Müller M. A., Eckerle I., Brünink S., Drosten C., Drexler J. F.. ( 2014; ). Characterization of a novel betacoronavirus related to middle East respiratory syndrome coronavirus in European hedgehogs. . J Virol 88:, 717–724. [CrossRef] [PubMed]
    [Google Scholar]
  7. Deng X. X., L., Ou Y. J., Su H. J., Li G., Guo Z. X., Zhang R., Zheng P. R., Chen Y. G.. & other authors ( 2012; ). Sequence analysis of 12 genome segments of mud crab reovirus (MCRV). . Virology 422:, 185–194. [CrossRef] [PubMed]
    [Google Scholar]
  8. Graham R. I., Rao S., Sait S. M., Attoui H., Mertens P. P., Hails R. S., Possee R. D.. ( 2008; ). Sequence analysis of a reovirus isolated from the winter moth Operophtera brumata (Lepidoptera: Geometridae) and its parasitoid wasp Phobocampe tempestiva (Hymenoptera: Ichneumonidae). . Virus Res 135:, 42–47. [CrossRef] [PubMed]
    [Google Scholar]
  9. Hillman B. I., Suzuki N.. ( 2004; ). Viruses of the chestnut blight fungus, Cryphonectria parasitica . . Adv Virus Res 63:, 423–472. [CrossRef] [PubMed]
    [Google Scholar]
  10. Hoffmann M., Müller M. A., Drexler J. F., Glende J., Erdt M., Gützkow T., Losemann C., Binger T., Deng H.. & other authors ( 2013; ). Differential sensitivity of bat cells to infection by enveloped RNA viruses: coronaviruses, paramyxoviruses, filoviruses, and influenza viruses. . PLoS ONE 8:, e72942. [CrossRef] [PubMed]
    [Google Scholar]
  11. Huang Z., Deng X., Li Y., Su H., Li K., Guo Z., Zheng P., Xu H., He J.. & other authors ( 2012; ). Structural insights into the classification of Mud Crab Reovirus. . Virus Res 166:, 116–120. [CrossRef] [PubMed]
    [Google Scholar]
  12. Junglen S., Kopp A., Kurth A., Pauli G., Ellerbrok H., Leendertz F. H.. ( 2009a; ). A new flavivirus and a new vector: characterization of a novel flavivirus isolated from uranotaenia mosquitoes from a tropical rain forest. . J Virol 83:, 4462–4468. [CrossRef] [PubMed]
    [Google Scholar]
  13. Junglen S., Kurth A., Kuehl H., Quan P. L., Ellerbrok H., Pauli G., Nitsche A., Nunn C., Rich S. M.. & other authors ( 2009b; ). Examining landscape factors influencing relative distribution of mosquito genera and frequency of virus infection. . EcoHealth 6:, 239–249. [CrossRef] [PubMed]
    [Google Scholar]
  14. Li Y., Tan L., Li Y., Chen W., Zhang J., Hu Y.. ( 2006; ). Identification and genome characterization of Heliothis armigera cypovirus types 5 and 14 and Heliothis assulta cypovirus type 14. . J Gen Virol 87:, 387–394. [CrossRef] [PubMed]
    [Google Scholar]
  15. Marklewitz M., Zirkel F., Rwego I. B., Heidemann H., Trippner P., Kurth A., Kallies R., Briese T., Lipkin W. I.. & other authors ( 2013; ). Discovery of a unique novel clade of mosquito-associated bunyaviruses. . J Virol 87:, 12850–12865. [CrossRef] [PubMed]
    [Google Scholar]
  16. Markussen T., Dahle M. K., Tengs T., Løvoll M., Finstad O. W., Wiik-Nielsen C. R., Grove S., Lauksund S., Robertsen B., Rimstad E.. ( 2013; ). Sequence analysis of the genome of piscine orthoreovirus (PRV) associated with heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar). . PLoS ONE 8:, e70075. [CrossRef] [PubMed]
    [Google Scholar]
  17. Nakashima N., Koizumi M., Watanabe H., Noda H.. ( 1996; ). Complete nucleotide sequence of the Nilaparvata lugens reovirus: a putative member of the genus Fijivirus . . J Gen Virol 77:, 139–146. [CrossRef] [PubMed]
    [Google Scholar]
  18. Nibert M. L., Kim J.. ( 2004; ). Conserved sequence motifs for nucleoside triphosphate binding unique to turreted reoviridae members and coltiviruses. . J Virol 78:, 5528–5530. [CrossRef] [PubMed]
    [Google Scholar]
  19. Poch O., Sauvaget I., Delarue M., Tordo N.. ( 1989; ). Identification of four conserved motifs among the RNA-dependent polymerase encoding elements. . EMBO J 8:, 3867–3874.[PubMed]
    [Google Scholar]
  20. Poch O., Blumberg B. M., Bougueleret L., Tordo N.. ( 1990; ). Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: theoretical assignment of functional domains. . J Gen Virol 71:, 1153–1162. [CrossRef] [PubMed]
    [Google Scholar]
  21. Quito-Avila D. F., Jelkmann W., Tzanetakis I. E., Keller K., Martin R. R.. ( 2011; ). Complete sequence and genetic characterization of Raspberry latent virus, a novel member of the family Reoviridae . . Virus Res 155:, 397–405. [CrossRef] [PubMed]
    [Google Scholar]
  22. Shao C. G., H. J., Wu J. H., Gong Z. X.. ( 2004; ). Nucleic acid binding activity of pns6 encoded by genome segment 6 of rice ragged stunt oryzavirus. . Acta Biochim Biophys Sin (Shanghai) 36:, 457–466. [CrossRef] [PubMed]
    [Google Scholar]
  23. Spear A., Sisterson M. S., Stenger D. C.. ( 2012; ). Reovirus genomes from plant-feeding insects represent a newly discovered lineage within the family Reoviridae . . Virus Res 163:, 503–511. [CrossRef] [PubMed]
    [Google Scholar]
  24. Stang A., Korn K., Wildner O., Uberla K.. ( 2005; ). Characterization of virus isolates by particle-associated nucleic acid PCR. . J Clin Microbiol 43:, 716–720. [CrossRef] [PubMed]
    [Google Scholar]
  25. Suzuki N., Supyani S., Maruyama K., Hillman B. I.. ( 2004; ). Complete genome sequence of Mycoreovirus-1/Cp9B21, a member of a novel genus within the family Reoviridae, isolated from the chestnut blight fungus Cryphonectria parasitica . . J Gen Virol 85:, 3437–3448. [CrossRef] [PubMed]
    [Google Scholar]
  26. Wu J., Du Z., Wang C., Cai L., Hu M., Lin Q., Wu Z., Li Y., Xie L.. ( 2010a; ). Identification of Pns6, a putative movement protein of RRSV, as a silencing suppressor. . Virol J 7:, 335. [CrossRef] [PubMed]
    [Google Scholar]
  27. Wu Z., Wu J., Adkins S., Xie L., Li W.. ( 2010b; ). Rice ragged stunt virus segment S6-encoded nonstructural protein Pns6 complements cell-to-cell movement of Tobacco mosaic virus-based chimeric virus. . Virus Res 152:, 176–179. [CrossRef] [PubMed]
    [Google Scholar]
  28. Yan J., Kudo H., Uyeda I., Lee S. Y., Shikata E.. ( 1992; ). Conserved terminal sequences of rice ragged stunt virus genomic RNA. . J Gen Virol 73:, 785–789. [CrossRef] [PubMed]
    [Google Scholar]
  29. Zirkel F., Roth H., Kurth A., Drosten C., Ziebuhr J., Junglen S.. ( 2013; ). Identification and characterization of genetically divergent members of the newly established family Mesoniviridae . . J Virol 87:, 6346–6358. [CrossRef] [PubMed]
    [Google Scholar]
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