1887

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Volume 95, Issue 3

Checks and balances between human cytomegalovirus replication and indoleamine-2,3-dioxygenase Free

Abstract

Despite a rigorous blockade of interferon-γ (IFN-γ) signalling in infected fibroblasts as a mechanism of immune evasion by human cytomegalovirus (HCMV), IFN-γ induced indoleamine-2,3-dioxygenase (IDO) has been proposed to represent the major antiviral restriction factor limiting HCMV replication in epithelial cells. Here we show that HCMV efficiently blocks transcription of IFN-γ-induced IDO mRNA both in infected fibroblasts and epithelial cells even in the presence of a preexisting IFN-induced antiviral state. This interference results in severe suppression of IDO bioactivity in HCMV-infected cells and restoration of vigorous HCMV replication. Depletion of IDO expression nonetheless substantially alleviated the antiviral impact of IFN-γ treatment in both cell types. These findings highlight the effectiveness of this IFN-γ induced effector gene in restricting HCMV productivity, but also the impact of viral counter-measures.

  • Received:
  • Accepted:
  • Published Online:
© 2014 SGM
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2014-03-01
2024-04-25
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Checks and balances between human cytomegalovirus replication and indoleamine-2,3-dioxygenase
J. Gen. Virol. 95, 659 (2014); https://doi.org/10.1099/vir.0.061994-0
/content/journal/jgv/10.1099/vir.0.061994-0
/content/journal/jgv/10.1099/vir.0.061994-0
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