1887

Abstract

The freshwater fish (bluegill) is common to North American waters, and important both ecologically and as a sport fish. In 2001 an unknown virus was isolated from bluegills following a bluegill fish kill. This virus was identified as a picornavirus [termed bluegill picornavirus (BGPV)] and a diagnostic reverse transcriptase PCR was developed. A survey of bluegills in Wisconsin waters showed the presence of BGPV in 5 of 17 waters sampled, suggesting the virus is widespread in bluegill populations. Experimental infections of bluegills confirmed that BGPV can cause morbidity and mortality in bluegills. Molecular characterization of BGPV revealed several distinct genome characteristics, the most unusual of which is the presence of a short poly(C) tract in the 3′ UTR. Additionally, the genome encodes a polyprotein lacking a leader peptide and a VP0 maturation cleavage site, and is predicted to encode two distinct 2A proteins. Sequence comparison showed that the virus is most closely related to a phylogenetic cluster of picornaviruses that includes the genera , and . However, it is distinct enough, for example sharing only about 38 % sequence identity to the parechoviruses in the 3D region, that it may represent a new genus in the family .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.061960-0
2014-03-01
2019-11-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/95/3/601.html?itemId=/content/journal/jgv/10.1099/vir.0.061960-0&mimeType=html&fmt=ahah

References

  1. Adams M. J., King A. M. Q., Carstens E. B.. ( 2013; ). Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2013). . Arch Virol 158:, 2023–2030. [CrossRef] [PubMed]
    [Google Scholar]
  2. American Fisheries Society ( 2009; ). Suggested Procedures for the Detection and Identification of Certain Finfish and Shellfish Pathogens, Blue Book 2009. Bethesda, MD:: American Fisheries Society;. [CrossRef]
    [Google Scholar]
  3. Boros Á., Nemes C., Pankovics P., Kapusinszky B., Delwart E., Reuter G.. ( 2013a; ). Genetic characterization of a novel picornavirus in turkeys (Meleagris gallopavo) distinct from turkey galliviruses and megriviruses and distantly related to the members of the genus Avihepatovirus . . J Gen Virol 94:, 1496–1509. [CrossRef] [PubMed]
    [Google Scholar]
  4. Boros Á., Kiss T., Kiss O., Pankovics P., Kapusinszky B., Delwart E., Reuter G.. ( 2013b; ). Genetic characterization of a novel picornavirus distantly related to the marine mammal-infecting aquamaviruses in a long-distance migrant bird species, European roller (Coracias garrulus). . J Gen Virol 94:, 2029–2035. [CrossRef] [PubMed]
    [Google Scholar]
  5. Dimmic M. W., Rest J. S., Mindell D. P., Goldstein R. A.. ( 2002; ). rtREV: an amino acid substitution matrix for inference of retrovirus and reverse transcriptase phylogeny. . J Mol Evol 55:, 65–73. [CrossRef] [PubMed]
    [Google Scholar]
  6. Ding C., Zhang D.. ( 2007; ). Molecular analysis of duck hepatitis virus type 1. . Virology 361:, 9–17. [CrossRef] [PubMed]
    [Google Scholar]
  7. Donnelly M. L., Luke G., Mehrotra A., Li X., Hughes L. E., Gani D., Ryan M. D.. ( 2001; ). Analysis of the aphthovirus 2A/2B polyprotein ‘cleavage’ mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal ‘skip’. . J Gen Virol 82:, 1013–1025.[PubMed]
    [Google Scholar]
  8. Duke G. M., Palmenberg A. C.. ( 1989; ). Cloning and synthesis of infectious cardiovirus RNAs containing short, discrete poly(C) tracts. . J Virol 63:, 1822–1826.[PubMed]
    [Google Scholar]
  9. Duke G. M., Osorio J. E., Palmenberg A. C.. ( 1990; ). Attenuation of Mengo virus through genetic engineering of the 5′ noncoding poly(C) tract. . Nature 343:, 474–476. [CrossRef] [PubMed]
    [Google Scholar]
  10. Edgar R. C.. ( 2004; ). MUSCLE: multiple sequence alignment with high accuracy and high throughput. . Nucleic Acids Res 32:, 1792–1797. [CrossRef] [PubMed]
    [Google Scholar]
  11. Fernández-Miragall O., Ramos R., Ramajo J., Martínez-Salas E.. ( 2006; ). Evidence of reciprocal tertiary interactions between conserved motifs involved in organizing RNA structure essential for internal initiation of translation. . RNA 12:, 223–234. [CrossRef] [PubMed]
    [Google Scholar]
  12. Fichtner D., Philipps A., Groth M., Schmidt-Posthaus H., Granzow H., Dauber M., Platzer M., Bergmann S. M., Schrudde D.. & other authors ( 2013; ). Characterization of a novel picornavirus isolate from a diseased European eel (Anguilla anguilla). . J Virol 87:, 10895–10899. [CrossRef] [PubMed]
    [Google Scholar]
  13. Ghazi F., Hughes P. J., Hyypiä T., Stanway G.. ( 1998; ). Molecular analysis of human parechovirus type 2 (formerly echovirus 23). . J Gen Virol 79:, 2641–2650.[PubMed]
    [Google Scholar]
  14. Goodwin A. E., Merry G. E.. ( 2011; ). Mortality and carrier status of bluegills exposed to viral hemorrhagic septicemia virus genotype IVb at different temperatures. . J Aquat Anim Health 23:, 85–91. [CrossRef] [PubMed]
    [Google Scholar]
  15. Gorbalenya A. E., Koonin E. V., Wolf Y. I.. ( 1990; ). A new superfamily of putative NTP-binding domains encoded by genomes of small DNA and RNA viruses. . FEBS Lett 262:, 145–148. [CrossRef] [PubMed]
    [Google Scholar]
  16. Greninger A. L., Knudsen G. M., Betegon M., Burlingame A. L., Derisi J. L.. ( 2012; ). The 3A protein from multiple picornaviruses utilizes the Golgi adaptor protein ACBD3 to recruit PI4KIIIβ. . J Virol 86:, 3605–3616. [CrossRef] [PubMed]
    [Google Scholar]
  17. Grizzle J. M., Brunner C. J.. ( 2003; ). Review of largemouth bass virus. . Fisheries 28:, 10–14. [CrossRef]
    [Google Scholar]
  18. Hughes P. J., Stanway G.. ( 2000; ). The 2A proteins of three diverse picornaviruses are related to each other and to the H-rev107 family of proteins involved in the control of cell proliferation. . J Gen Virol 81:, 201–207.[PubMed]
    [Google Scholar]
  19. Jang S. K.. ( 2006; ). Internal initiation: IRES elements of picornaviruses and hepatitis C virus. . Virus Res 119:, 2–15. [CrossRef] [PubMed]
    [Google Scholar]
  20. Johansson S., Niklasson B., Maizel J., Gorbalenya A. E., Lindberg A. M.. ( 2002; ). Molecular analysis of three Ljungan virus isolates reveals a new, close-to-root lineage of the Picornaviridae with a cluster of two unrelated 2A proteins. . J Virol 76:, 8920–8930. [CrossRef] [PubMed]
    [Google Scholar]
  21. Kadaré G., Haenni A. L.. ( 1997; ). Virus-encoded RNA helicases. . J Virol 71:, 2583–2590.[PubMed]
    [Google Scholar]
  22. Kaminski A., Hunt S. L., Gibbs C. L., Jackson R. J.. ( 1994; ). Internal initiation of mRNA translation in eukaryotes. . Genet Eng (N Y) 16:, 115–155.[PubMed]
    [Google Scholar]
  23. Kapoor A., Victoria J., Simmonds P., Wang C., Shafer R. W., Nims R., Nielsen O., Delwart E.. ( 2008; ). A highly divergent picornavirus in a marine mammal. . J Virol 82:, 311–320. [CrossRef] [PubMed]
    [Google Scholar]
  24. Knowles N. J.. ( 2005; ). A pan-picornavirus RT-PCR: identification of novel picornavirus species. . In EUROPIC 2005: XIIIth Meeting of the European Study Group on the Molecular Biology of Picornaviruses. Lunteren, The Netherlands:.
    [Google Scholar]
  25. Knowles N. J., Hovi T., King A. M. Q., Stanway G.. ( 2010; ). Overview of taxonomy. . In The Picornaviruses, pp. 19–32. Edited by Ehrenfeld, E. D. E., Domingo E., Roos. R. P.. Washington, DC:: American Society for Microbiology;. [CrossRef]
    [Google Scholar]
  26. Knowles N. J., Hovi T., Hyypiä T., King A. M. Q., Lindberg A. M., Pallansch M. A., Palmenberg A. C., Simmonds P., Skern T.. & other authors ( 2012; ). Picornaviridae. . In Virus Taxonomy: Classification and Nomenclature of Viruses: Ninth Report of the International Committee on Taxonomy of Viruses, pp. 855–880. Edited by King A. M. Q., Adams M. J., Carstens E. B. , Lefkowitz E. J... San Diego, CA:: Elsevier;.
    [Google Scholar]
  27. Lange J., Groth M., Fichtner D., Granzow H., Keller B., Walther M., Platzer M., Sauerbrei A., Zell R.. ( 2013; ). Virus isolate from carp: genetic characterization reveals a novel picornavirus with two aphthovirus 2A-like sequences. . J Gen Virol 95:, 80–90. [CrossRef] [PubMed]
    [Google Scholar]
  28. López de Quinto S., Martínez-Salas E.. ( 1997; ). Conserved structural motifs located in distal loops of aphthovirus internal ribosome entry site domain 3 are required for internal initiation of translation. . J Virol 71:, 4171–4175.[PubMed]
    [Google Scholar]
  29. Meyers T. R., Hirai K.. ( 1980; ). Morphology of a reo-like virus isolated from juvenile American oysters (Crassotrea virginica). . J Gen Virol 46:, 249–253. [CrossRef] [PubMed]
    [Google Scholar]
  30. Michigan Department of Natural Resources ( 2007; ). Fish disease discovered in Budd Lake, Clare County. Press Release. . Newberry, MI:: Michigan Department of Natural Resources;.
  31. Ng T. F., Cheung A. K., Wong W., Lager K. M., Kondov N. O., Cha Y., Murphy D. A., Pogranichniy R. M., Delwart E.. ( 2013; ). Divergent picornavirus from a turkey with gastrointestinal disease. . Genome Announc 1:, e00134-13. [CrossRef] [PubMed]
    [Google Scholar]
  32. Palmenberg A., Neubauer D., Skern T.. ( 2010; ). Genome organization and encoded proteins. . In The Picornaviruses, pp. 3–17. Edited by Ehrenfeld E. D. E., Domingo E., Roos R. P... Washington, DC:: American Society for Microbiology;. [CrossRef]
    [Google Scholar]
  33. Robertson M. E., Seamons R. A., Belsham G. J.. ( 1999; ). A selection system for functional internal ribosome entry site (IRES) elements: analysis of the requirement for a conserved GNRA tetraloop in the encephalomyocarditis virus IRES. . RNA 5:, 1167–1179. [CrossRef] [PubMed]
    [Google Scholar]
  34. Rottmann R. W., Francis-Floyd R., Durborow R.. ( 1992; ). The Role of Stress in Fish Disease (Southern Regional Aquaculture Center Publication, no. 474). College Station, TX:: Texas A. & M. University;.
    [Google Scholar]
  35. Ryan M. D., Donnelly M., Lewis A., Mehrotra A. P., Wilkie J., Gani D.. ( 1999; ). A model for nonstoichiometric, cotranslational protein scission in eukaryotic ribosomes. . Bioorg Chem 27:, 55–79. [CrossRef]
    [Google Scholar]
  36. Sauvage V., Ar Gouilh M., Cheval J., Muth E., Pariente K., Burguiere A., Caro V., Manuguerra J. C., Eloit M.. ( 2012; ). A member of a new Picornaviridae genus is shed in pig feces. . J Virol 86:, 10036–10046. [CrossRef] [PubMed]
    [Google Scholar]
  37. Snow H. E., Ensign A., Klingbiel J.. ( 1978; ). The Bluegill: its Life History, Ecology and Management. Madison, WI:: Department of Natural Resources;.
    [Google Scholar]
  38. Subramaniam S.. ( 1998; ). The Biology Workbench–a seamless database and analysis environment for the biologist. . Proteins 32:, 1–2. [CrossRef] [PubMed]
    [Google Scholar]
  39. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011; ). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  40. Toyoda H., Franco D., Fujita K., Paul A. V., Wimmer E.. ( 2007; ). Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. . J Virol 81:, 10017–10028. [CrossRef] [PubMed]
    [Google Scholar]
  41. Tseng C. H., Knowles N. J., Tsai H. J.. ( 2007; ). Molecular analysis of duck hepatitis virus type 1 indicates that it should be assigned to a new genus. . Virus Res 123:, 190–203. [CrossRef] [PubMed]
    [Google Scholar]
  42. Wolf K., Carlson C. P.. ( 1965; ). Multiplication of lymphocystis virus in the bluegill (Lepomis macrochirus). . Ann N Y Acad Sci 126:, 414–419. [CrossRef] [PubMed]
    [Google Scholar]
  43. Wu Z., Ren X., Yang L., Hu Y., Yang J., He G., Zhang J., Dong J., Sun L.. & other authors ( 2012; ). Virome analysis for identification of novel mammalian viruses in bat species from Chinese provinces. . J Virol 86:, 10999–11012. [CrossRef] [PubMed]
    [Google Scholar]
  44. Yu J. M., Li J. S., Ao Y. Y., Duan Z. J.. ( 2013; ). Detection of novel viruses in porcine fecal samples from China. . Virol J 10:, 39. [CrossRef] [PubMed]
    [Google Scholar]
  45. Zuker M.. ( 2003; ). Mfold web server for nucleic acid folding and hybridization prediction. . Nucleic Acids Res 31:, 3406–3415. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.061960-0
Loading
/content/journal/jgv/10.1099/vir.0.061960-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error