1887

Abstract

Infection of the lepidopteran insect with the baculovirus M nucleopolyhedrovirus (AcMNPV) by the oral route stimulates activation of host matrix metalloproteases (MMP) and effector caspases, a process dependent on expression of the viral fibroblast growth factor (vFGF). This pathway leads to tracheal cell basal lamina remodelling, enabling virus escape from the primary site of infection, the midgut epithelium, and establishment of efficient systemic infection. In this study, we asked whether the MMP–caspase pathway was also activated following infection by intrahaemocoelic injection. We found that intrahaemocoelic infection did not lead to any observable tracheal cell or midgut epithelium basal lamina remodelling. MMP and caspase activities were not significantly stimulated. We conclude that the main role of the AcMNPV vFGF is in facilitating virus midgut escape.

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/content/journal/jgv/10.1099/vir.0.060517-0
2014-03-01
2024-04-24
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References

  1. Ayres M. D., Howard S. C., Kuzio J., López-Ferber M., Possee R. D. 1994; The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202:586–605 [View Article][PubMed]
    [Google Scholar]
  2. Chen Y.-R., Wu C.-Y., Lee S.-T., Wu Y.-J., Lo C.-F., Tsai M.-F., Wang C.-H. 2008; Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus. J Gen Virol 89:2315–2330 [View Article][PubMed]
    [Google Scholar]
  3. Detvisitsakun C., Cain E. L., Passarelli A. L. 2007; The Autographa californica M nucleopolyhedrovirus fibroblast growth factor accelerates host mortality. Virology 365:70–78 [View Article][PubMed]
    [Google Scholar]
  4. Engelhard E. K., Kam-Morgan L. N. W., Washburn J. O., Volkman L. E. 1994; The insect tracheal system: a conduit for the systemic spread of Autographa californica M nuclear polyhedrosis virus. Proc Natl Acad Sci U S A 91:3224–3227 [View Article][PubMed]
    [Google Scholar]
  5. Florentin A., Arama E. 2012; Caspase levels and execution efficiencies determine the apoptotic potential of the cell. J Cell Biol 196:513–527 [View Article][PubMed]
    [Google Scholar]
  6. Grose R., Dickson C. 2005; Fibroblast growth factor signaling in tumorigenesis. Cytokine Growth Factor Rev 16:179–186 [View Article][PubMed]
    [Google Scholar]
  7. Katsuma S., Horie S., Shimada T. 2008; The fibroblast growth factor homolog of Bombyx mori nucleopolyhedrovirus enhances systemic virus propagation in B. mori larvae. Virus Res 137:80–85 [View Article][PubMed]
    [Google Scholar]
  8. Means J. C., Passarelli A. L. 2010; Viral fibroblast growth factor, matrix metalloproteases, and caspases are associated with enhancing systemic infection by baculoviruses. Proc Natl Acad Sci U S A 107:9825–9830 [View Article][PubMed]
    [Google Scholar]
  9. Nhan T. Q., Liles W. C., Schwartz S. M. 2006; Physiological functions of caspases beyond cell death. Am J Pathol 169:729–737 [View Article][PubMed]
    [Google Scholar]
  10. Ornitz D. M., Itoh N. 2001; Fibroblast growth factors. Genome Biol 2:3005–, 3012 [View Article][PubMed]
    [Google Scholar]
  11. Passarelli A. L. 2011; Barriers to success: how baculoviruses establish efficient systemic infections. Virology 411:383–392 [View Article][PubMed]
    [Google Scholar]
  12. Rohrmann G. F. 2011 Baculovirus Molecular Biology Bethesda, MD: National Center for Biotechnology Information;
    [Google Scholar]
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