Here we describe the design and strength of a new synthetic late-early optimized (LEO) vaccinia virus (VACV) promoter used as a transcriptional regulator of GFP expression during modified vaccinia Ankara infection. In contrast to the described synthetic VACV promoter (pS), LEO induced significantly higher levels of GFP expression in vitro within the first hour after infection, which correlated with an enhancement in the GFP-specific CD8 T-cell response detected in vivo, demonstrating its potential use in future vaccines.
AssarssonE.,
GreenbaumJ. A.,
SundströmM.,
SchafferL.,
HammondJ. A.,
PasquettoV.,
OseroffC.,
HendricksonR. C.,
LefkowitzE. J.other authors2008; Kinetic analysis of a complete poxvirus transcriptome reveals an immediate-early class of genes. Proc Natl Acad Sci U S A 105:2140–2145 [View Article][PubMed]
ChakrabartiS.,
SislerJ. R.,
MossB.1997; Compact, synthetic, vaccinia virus early/late promoter for protein expression. Biotechniques 23:1094–1097[PubMed]
GambottoA.,
DworackiG.,
CicinnatiV.,
KennistonT.,
SteitzJ.,
TütingT.,
RobbinsP. D.,
DeLeoA. B.2000; Immunogenicity of enhanced green fluorescent protein (EGFP) in BALB/c mice: identification of an H2-Kd-restricted CTL epitope. Gene Ther 7:2036–2040 [View Article][PubMed]
García-ArriazaJ.,
NájeraJ. L.,
GómezC. E.,
SorzanoC. O.,
EstebanM.2010; Immunogenic profiling in mice of a HIV/AIDS vaccine candidate (MVA-B) expressing four HIV-1 antigens and potentiation by specific gene deletions. PLoS ONE 5:e12395 [View Article][PubMed]
GómezC. E.,
NájeraJ. L.,
JiménezE. P.,
JiménezV.,
WagnerR.,
GrafM.,
FrachetteM. J.,
LiljeströmP.,
PantaleoG.,
EstebanM.2007a; Head-to-head comparison on the immunogenicity of two HIV/AIDS vaccine candidates based on the attenuated poxvirus strains MVA and NYVAC co-expressing in a single locus the HIV-1BX08 gp120 and HIV-1(IIIB) Gag-Pol-Nef proteins of clade B. Vaccine 25:2863–2885 [View Article][PubMed]
GómezC. E.,
PerdigueroB.,
JiménezV.,
Filali-MouhimA.,
GhneimK.,
HaddadE. K.,
QuakkelaarE. D.,
DelaloyeJ.,
HarariA.other authors2012b; Systems analysis of MVA-C induced immune response reveals its significance as a vaccine candidate against HIV/AIDS of clade C. PLoS ONE 7:e35485 [View Article][PubMed]
HonessR. W.,
RoizmanB.1975; Regulation of herpesvirus macromolecular synthesis: sequential transition of polypeptide synthesis requires functional viral polypeptides. Proc Natl Acad Sci U S A 72:1276–1280 [View Article][PubMed]
OrubuT.,
AlharbiN. K.,
LambeT.,
GilbertS. C.,
CottinghamM. G.2012; Expression and cellular immunogenicity of a transgenic antigen driven by endogenous poxviral early promoters at their authentic loci in MVA. PLoS ONE 7:e40167 [View Article][PubMed]
RamírezJ. C.,
GherardiM. M.,
EstebanM.2000; Biology of attenuated modified vaccinia virus Ankara recombinant vector in mice: virus fate and activation of B- and T-cell immune responses in comparison with the Western Reserve strain and advantages as a vaccine. J Virol 74:923–933 [View Article][PubMed]
SalserW.,
BolleA.,
EpsteinR.1970; Transcription during bacteriophage T4 development: a demonstration that distinct subclasses of the “early” RNA appear at different times and that some are “turned off” at late times. J Mol Biol 49:271–295 [View Article][PubMed]
Sánchez-SampedroL.,
GómezC. E.,
Mejías-PérezE.,
SorzanoC. O. S.,
EstebanM.2012; High quality long-term CD4+ and CD8+ effector memory populations stimulated by DNA-LACK/MVA-LACK regimen in Leishmania major BALB/c model of infection. PLoS ONE 7:e38859 [View Article][PubMed]
SetteA.,
GreyH.,
OseroffC.,
PetersB.,
MoutaftsiM.,
CrottyS.,
AssarssonE.,
GreenbaumJ.,
KimY.other authors2009; Definition of epitopes and antigens recognized by vaccinia specific immune responses: their conservation in variola virus sequences, and use as a model system to study complex pathogens. Vaccine 27:Suppl 6G21–G26 [View Article][PubMed]
VijayanA.,
GómezC. E.,
EspinosaD. A.,
GoodmanA. G.,
Sanchez-SampedroL.,
SorzanoC. O.,
ZavalaF.,
EstebanM.2012; Adjuvant-like effect of vaccinia virus 14K protein: a case study with malaria vaccine based on the circumsporozoite protein. J Immunol 188:6407–6417 [View Article][PubMed]
YangZ.,
BrunoD. P.,
MartensC. A.,
PorcellaS. F.,
MossB.2010; Simultaneous high-resolution analysis of vaccinia virus and host cell transcriptomes by deep RNA sequencing. Proc Natl Acad Sci U S A 107:11513–11518 [View Article][PubMed]