RT Journal Article SR Electronic(1) A1 Di Pilato, Mauro A1 Mejías-Pérez, Ernesto A1 Gómez, Carmen Elena A1 Perdiguero, Beatriz A1 Sorzano, Carlos Oscar S. A1 Esteban, MarianoYR 2013 T1 New vaccinia virus promoter as a potential candidate for future vaccines JF Journal of General Virology, VO 94 IS 12 SP 2771 OP 2776 DO https://doi.org/10.1099/vir.0.057299-0 PB Microbiology Society, SN 1465-2099, AB Here we describe the design and strength of a new synthetic late-early optimized (LEO) vaccinia virus (VACV) promoter used as a transcriptional regulator of GFP expression during modified vaccinia Ankara infection. In contrast to the described synthetic VACV promoter (pS), LEO induced significantly higher levels of GFP expression in vitro within the first hour after infection, which correlated with an enhancement in the GFP-specific CD8 T-cell response detected in vivo, demonstrating its potential use in future vaccines., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.057299-0