1887

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Volume 94, Issue 10

Identification and genomic characterization of a novel fish reovirus, Hubei grass carp disease reovirus, isolated in 2009 in China Free

Abstract

A novel fish reovirus, Hubei grass carp disease reovirus (HGDRV; formerly grass carp reovirus strain 104, GCRV104), was isolated from diseased grass carp in China in 2009 and the full genome sequence was determined. This reovirus was propagated in a grass carp kidney cell line with a typical cytopathic effect. The total size of the genome was 23 706 bp with a 51 mol% G+C content, and the 11 dsRNA segments encoded 12 proteins (two proteins encoded by segment 11). A nucleotide sequence similarity search using found no significant matches except for segment 2, which partially matched that of the RNA-dependent RNA polymerase (RdRp) from several viruses in the genera and of the family . At the amino acid level, seven segments (Seg-1 to Seg-6, and Seg-8) matched with species in the genera (15–46 % identities) and (12–44 % identities), while for four segments (Seg-7, Seg-9, Seg-10 and Seg-11) no similarities in these genera were found. Conserved terminal sequences, 5′-GAAUU----UCAUC-3′, were found in each HGDRV segment at the 5′ and 3′ ends, and the 5′-terminal nucleotides were different from any known species in the genus . Phylogenetic analysis based on RdRp amino acid sequences from members of the family showed that HGDRV clustered with aquareoviruses prior to joining a branch common with orthoreoviruses. Based on these observations, we propose that HGDRV is a new species in the genus that is distantly related to any known species within this genus.

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  • Accepted:
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© 2013 SGM
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2013-10-01
2024-04-20
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Identification and genomic characterization of a novel fish reovirus, Hubei grass carp disease reovirus, isolated in 2009 in China
J. Gen. Virol. 94, 2266 (2013); https://doi.org/10.1099/vir.0.054767-0
/content/journal/jgv/10.1099/vir.0.054767-0
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