1887

Abstract

Deep-sequencing analysis of nucleic acids from leaf tissue of Japanese persimmon trees exhibiting fruit apex disorder in some fruits detected two molecules that were graft transmitted to healthy seedlings. One of the complete genomes consisted of 13 467 nt and encoded six genes similar to those of plant rhabdoviruses. The virus formed a distinct cluster in the genus with lettuce necrotic yellows virus, lettuce yellow mottle virus and strawberry crinkle virus in a phylogenetic tree based on the L protein (RNA-dependent RNA polymerase, RdRp). The other consisted of 7475 nt and shared a genome organization similar to those of some insect and fungal viruses having dsRNA genomes. In a phylogenetic tree using the RdRp sequence of several unassigned dsRNA viruses, the virus formed a possible new genus cluster with two insect viruses, virus 1 and virus 1, and one plant virus, cucurbit yellows-associated virus.

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2013-08-01
2024-12-12
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References

  1. Al Rwahnih M., Daubert S., Golino D., Rowhani A. 2009; Deep sequencing analysis of RNAs from a grapevine showing Syrah decline symptoms reveals a multiple virus infection that includes a novel virus. Virology 387:395–401 [View Article][PubMed]
    [Google Scholar]
  2. Coffin R. S., Coutts R. H. A. 1995; Relationships among Trialeurodes vaporariorum-transmitted yellowing viruses from Europe and North America. J Phytopathol 143:375–380 [View Article]
    [Google Scholar]
  3. Dietzgen R. G., Callaghan B., Wetzel T., Dale J. L. 2006; Completion of the genome sequence of Lettuce necrotic yellows virus, type species of the genus Cytorhabdovirus . Virus Res 118:16–22 [View Article][PubMed]
    [Google Scholar]
  4. Dietzgen R. G., Calisher C. H., Kurath G., Kuzmin I. V., Rodriguez L. L., Stone D. M., Tesh R. B., Tordo N., Walker P. J.& other authors ( 2012; Family Rhabdoviridae. In Virus Taxonomy, Ninth Report of the International Committee on Taxonomy of Viruses pp. 686–713 Edited by King A. M. Q., Adams M. J., Carstens E. B., Lefkowitz E. J. San Diego, CA: Academic Press;
    [Google Scholar]
  5. Heim F., Lot H., Delecolle B., Bassler A., Krczal G., Wetzel T. 2008; Complete nucleotide sequence of a putative new cytorhabdovirus infecting lettuce. Arch Virol 153:81–92 [View Article][PubMed]
    [Google Scholar]
  6. Imada J. 2000; Fruit apex disorder, a graft-transmissible of Japanese persimmon. Ann Phytopathological Soc Jpn 66:148 (abstract in Japanese)
    [Google Scholar]
  7. Kreuze J. F., Perez A., Untiveros M., Quispe D., Fuentes S., Barker I., Simon R. 2009; Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: a generic method for diagnosis, discovery and sequencing of viruses. Virology 388:1–7 [View Article][PubMed]
    [Google Scholar]
  8. Kusano N., Shimomura K. 1994; Occurrence of a graft-transmissible disease of persimmon. Ann Phytopathological Soc Jpn 60:739 (abstract in Japanese)
    [Google Scholar]
  9. Moon S., Byun Y., Kim H.-J., Jeong S., Han K. 2004; Predicting genes expressed via −1 and +1 frameshifts. Nucleic Acids Res 32:4884–4892 [View Article][PubMed]
    [Google Scholar]
  10. Morelli M., De Stradis A., La Notte P., Merkuri J., Boscia D., Minafra A. 2012; Detection and molecular characterization of a novel Cryptovirus from persimmon (Diospyros kaki). In Abstracts, 22nd International Conference on Virus and Other Transmissible Diseases of Fruit Crops p. 117 Rome, Italy: ICVF;
    [Google Scholar]
  11. Nakaune R., Nakano M. 2006; Efficient methods for sample processing and cDNA synthesis by RT-PCR for the detection of grapevine viruses and viroids. J Virol Methods 134:244–249 [View Article][PubMed]
    [Google Scholar]
  12. Nakaune R., Nakano M. 2008; Identification of a new Apscaviroid from Japanese persimmon. Arch Virol 153:969–972 [View Article][PubMed]
    [Google Scholar]
  13. Reid K. E., Olsson N., Schlosser J., Peng F., Lund S. T. 2006; An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development. BMC Plant Biol 6:27 [View Article][PubMed]
    [Google Scholar]
  14. Scott S. W., Payne J. A. 1988; Decline of oriental persimmon. Phytopathology 78:1568
    [Google Scholar]
  15. Shimizu S., Ito T., Miyoshi T., Tachibana Y., Ito T. 2011; A broad spectrum, one-step RT-PCR to detect Satsuma dwarf virus variants using universal primers targeting both segmented RNAs 1 and 2. J Gen Plant Pathol 77:326–330 [View Article]
    [Google Scholar]
  16. Spear A., Sisterson M. S., Yokomi R., Stenger D. C. 2010; Plant-feeding insects harbor double-stranded RNA viruses encoding a novel proline-alanine rich protein and a polymerase distantly related to that of fungal viruses. Virology 404:304–311 [View Article][PubMed]
    [Google Scholar]
  17. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  18. Wu Q., Luo Y., Lu R., Lau N., Lai E. C., Li W.-X., Ding S.-W. 2010; Virus discovery by deep sequencing and assembly of virus-derived small silencing RNAs. Proc Natl Acad Sci U S A 107:1606–1611 [View Article][PubMed]
    [Google Scholar]
  19. Yanase H., Umemoto S., Kume Y., Saigusa M. 1992; Occurrence of a new graft-transmissible disease of Kaki, Kaki dwarf. Ann Phytopathological Soc Jpn 58:618–619 (abstract in Japanese)
    [Google Scholar]
  20. Zerbino D. R., Birney E. 2008; Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18:821–829 [View Article][PubMed]
    [Google Scholar]
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