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Journal of General Virology header logo Journal of General Virology

Volume 94, Issue 10

Overexpression of microRNA gga-miR-1650 decreases the replication of avian leukosis virus subgroup J in infected cells No Access

Abstract

MicroRNAs (miRNAs) are a class of small regulatory non-coding RNAs that modulate gene expression at the post-transcriptional level, playing a crucial role in cell differentiation and development. Recently, some reports have demonstrated that a number of cellular miRNAs play a role during viral infection. In this study, a luciferase-reporter system carrying the 5′ untranslated region (5′ UTR) and 3′ UTR of avian leukosis virus subgroup J (ALV-J) was used to determine whether cellular miRNAs are involved in ALV-J infection. The miRNA gga-miR-1650 was screened for its potential interaction with the 5′ UTR of ALV-J and the ability to suppress luciferase-reporter activity. A mutational analysis of predicted gga-miR-1650-binding sites showed that the 5′ and 3′ ends of gga-miR-1650 contributed to the interaction between gga-miR-1650 and its target located at the 5′ UTR. Overexpression of miRNA gga-miR-1650 was shown to downregulate the expression of the Gag protein and influence the replication of ALV-J through binding to the 5′ UTR. Overall, this report provides the basis for the development of new strategies for anti-ALV-J intervention.

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© 2013 SGM
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/content/journal/jgv/10.1099/vir.0.054007-0
2013-10-01
2025-04-23
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Overexpression of microRNA gga-miR-1650 decreases the replication of avian leukosis virus subgroup J in infected cells
J. Gen. Virol. 94, 2287 (2013); https://doi.org/10.1099/vir.0.054007-0
/content/journal/jgv/10.1099/vir.0.054007-0
/content/journal/jgv/10.1099/vir.0.054007-0
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