1887

Abstract

Cell lines derived from the silkworm, , are only permissive for nucleopolyhedrovirus (NPV), with other NPVs generally resulting in abortive infection. Here, we demonstrate that rRNA of BM-N cells undergoes rapid degradation through site-specific cleavage upon infection with NPVs from (AcMNPV), (HycuMNPV), (SeMNPV) and (SpltMNPV). No significant decreases in cellular RNA were observed in Ld652Y, Se301, Sf9, SpIm and S2 cells infected with AcMNPV or HycuMNPV, indicating the response is unique to BM-N cells. A transient expression assay using a cosmid library of the HycuMNPV genome demonstrated that HycuMNPV P143 is responsible for rRNA degradation, which was also detected in BM-N cells transfected with plasmids expressing the P143 proteins from AcMNPV, SeMNPV and SpltMNPV. These results indicate that evolved to acquire a unique antiviral immune mechanism that is activated by P143 proteins from heterologous NPVs.

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2013-09-01
2019-10-17
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