1887

Abstract

Hepatitis B virus (HBV) DNA recombinants contribute to ~30 % of the overall full-length sequences already deposited in GenBank. However, their biological behaviour has not been analysed so far. In this study, the replication kinetics of the first D/A recombinant from the American continent differed from its parental genotypes, exhibiting higher extracellular levels of HBV DNA and hepatitis B e antigen. Southern blots of intracellular core-associated HBV DNA were in agreement with such results. Because this recombinant was obtained from an Argentinian injecting drug user belonging to a vulnerable community, these results are of singular relevance for regional public health. Further studies are urgently needed to determine the pathogenicity of these replicative competent clones.

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2013-12-01
2019-10-15
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References

  1. Baumert T. F., Rogers S. A., Hasegawa K., Liang T. J.. ( 1996;). Two core promotor mutations identified in a hepatitis B virus strain associated with fulminant hepatitis result in enhanced viral replication. . J Clin Invest 98:, 2268–2276. [CrossRef][PubMed]
    [Google Scholar]
  2. Chan H. L., Thompson A., Martinot-Peignoux M., Piratvisuth T., Cornberg M., Brunetto M. R., Tillmann H. L., Kao J. H., Jia J. D.. & other authors ( 2011;). Hepatitis B surface antigen quantification: why and how to use it in 2011: a core group report. . J Hepatol 55:, 1121–1131. [CrossRef][PubMed]
    [Google Scholar]
  3. Günther S., Piwon N., Iwanska A., Schilling R., Meisel H., Will H.. ( 1996;). Type, prevalence, and significance of core promoter/enhancer II mutations in hepatitis B viruses from immunosuppressed patients with severe liver disease. . J Virol 70:, 8318–8331.[PubMed]
    [Google Scholar]
  4. Kramvis A., Kew M. C.. ( 2005;). Relationship of genotypes of hepatitis B virus to mutations, disease progression and response to antiviral therapy. . J Viral Hepat 12:, 456–464. [CrossRef][PubMed]
    [Google Scholar]
  5. Lin C. L., Kao J. H.. ( 2011;). The clinical implications of hepatitis B virus genotype: recent advances. . J Gastroenterol Hepatol 26: (Suppl 1), 123–130. [CrossRef][PubMed]
    [Google Scholar]
  6. Liu C. J., Kao J. H., Chen D. S.. ( 2005;). Therapeutic implications of hepatitis B virus genotypes. . Liver Int 25:, 1097–1107. [CrossRef][PubMed]
    [Google Scholar]
  7. Pollicino T., Amaddeo G., Restuccia A., Raffa G., Alibrandi A., Cutroneo G., Favaloro A., Maimone S., Squadrito G., Raimondo G.. ( 2012;). Impact of hepatitis B virus (HBV) preS/S genomic variability on HBV surface antigen and HBV DNA serum levels. . Hepatology 56:, 434–443. [CrossRef][PubMed]
    [Google Scholar]
  8. Raimondo G., Allain J. P., Brunetto M. R., Buendia M. A., Chen D. S., Colombo M., Craxì A., Donato F., Ferrari C.. & other authors ( 2008;). Statements from the Taormina expert meeting on occult hepatitis B virus infection. . J Hepatol 49:, 652–657. [CrossRef][PubMed]
    [Google Scholar]
  9. Shi W., Carr M. J., Dunford L., Zhu C., Hall W. W., Higgins D. G.. ( 2012;). Identification of novel inter-genotypic recombinants of human hepatitis B viruses by large-scale phylogenetic analysis. . Virology 427:, 51–59. [CrossRef][PubMed]
    [Google Scholar]
  10. Sugiyama M., Tanaka Y., Kato T., Orito E., Ito K., Acharya S. K., Gish R. G., Kramvis A., Shimada T.. & other authors ( 2006;). Influence of hepatitis B virus genotypes on the intra- and extracellular expression of viral DNA and antigens. . Hepatology 44:, 915–924. [CrossRef][PubMed]
    [Google Scholar]
  11. Tacke F., Liedtke C., Bocklage S., Manns M. P., Trautwein C.. ( 2005;). CREB/PKA sensitive signalling pathways activate and maintain expression levels of the hepatitis B virus pre-S2/S promoter. . Gut 54:, 1309–1317. [CrossRef][PubMed]
    [Google Scholar]
  12. Trinks J., Cuestas M. L., Tanaka Y., Mathet V. L., Minassian M. L., Rivero C. W., Benetucci J. A., Gímenez E. D., Segura M.. & other authors ( 2008;). Two simultaneous hepatitis B virus epidemics among injecting drug users and men who have sex with men in Buenos Aires, Argentina: characterization of the first D/A recombinant from the American continent. . J Viral Hepat 15:, 827–838.[PubMed]
    [Google Scholar]
  13. Yuh C. H., Chang Y. L., Ting L. P.. ( 1992;). Transcriptional regulation of precore and pregenomic RNAs of hepatitis B virus. . J Virol 66:, 4073–4084.[PubMed]
    [Google Scholar]
  14. Zhou B., Wang Z., Yang J., Sun J., Li H., Tanaka Y., Mizokami M., Hou J.. ( 2012;). Novel evidence of HBV recombination in family cluster infections in western China. . PLoS ONE 7:, e38241. [CrossRef][PubMed]
    [Google Scholar]
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