1887

Abstract

Environmental and infection variables may affect the genetic diversity of baculovirus populations. In this study, nucleopolyhedrovirus (HearNPV) was used as a model system for studying the effects of a key infection variable, inoculum dose, on the genetic diversity within nucleopolyhedrovirus populations. Diversity and equitability indices were calculated from -specific denaturing gradient gel electrophoresis profiles obtained from individual neonate larvae inoculated with either an LD or LD of HearNPV. Although the genetic diversity detected in larvae treated with an LD was not statistically different from the diversity detected in the HearNPV inoculum samples, there was a statistically significant difference in the genetic diversity detected in the LD-inoculated larvae compared with the genetic diversity detected in the HearNPV samples used for the inoculations. The study suggests that inoculum dose needs to be considered carefully in experiments that evaluate HearNPV genetic diversity or in studies where differences in genetic diversity may have phenotypic consequences.

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2013-11-01
2020-10-20
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References

  1. Baillie V. L., Bouwer G. 2011; Development of highly sensitive assays for detection of genetic variation in key Helicoverpa armigera nucleopolyhedrovirus genes. J Virol Methods 178:179–185 [CrossRef][PubMed]
    [Google Scholar]
  2. Baillie V. L., Bouwer G. 2012a; High levels of genetic variation within core Helicoverpa armigera nucleopolyhedrovirus genes. Virus Genes 44:149–162 [CrossRef][PubMed]
    [Google Scholar]
  3. Baillie V. L., Bouwer G. 2012b; High levels of genetic variation within Helicoverpa armigera nucleopolyhedrovirus populations in individual host insects. Arch Virol 157:2281–2289 [CrossRef][PubMed]
    [Google Scholar]
  4. Bull J. C., Godfray H. C. J., O’Reilly D. R. 2001; Persistence of an occlusion-negative recombinant nucleopolyhedrovirus in Trichoplusia ni indicates high multiplicity of cellular infection. Appl Environ Microbiol 67:5204–5209 [CrossRef][PubMed]
    [Google Scholar]
  5. Chen X., Ijkel W. F. J., Tarchini R., Sun X., Sandbrink H., Wang H., Peters S., Zuidema D., Lankhorst R. K. other authors 2001; The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome. J Gen Virol 82:241–257[PubMed]
    [Google Scholar]
  6. Clavijo G., Williams T., Simón O., Muñoz D., Cerutti M., López-Ferber M., Caballero P. 2009; Mixtures of complete and pif1- and pif2-deficient genotypes are required for increased potency of an insect nucleopolyhedrovirus. J Virol 83:5127–5136 [CrossRef][PubMed]
    [Google Scholar]
  7. Cory J. S., Green B. M., Paul R. K., Hunter-Fujita F. 2005; Genotypic and phenotypic diversity of a baculovirus population within an individual insect host. J Invertebr Pathol 89:101–111 [CrossRef][PubMed]
    [Google Scholar]
  8. Croizier G., Ribeiro H. C. T. 1992; Recombination as a possible major cause of genetic heterogeneity in Anticarsia gemmatalis nuclear polyhedrosis virus wild populations. Virus Res 26:183–196 [CrossRef]
    [Google Scholar]
  9. Croizier G., Croizier L., Quiot J. M., Lereclus D. 1988; Recombination of Autographa californica and Rachiplusia ou nuclear polyhedrosis viruses in Galleria mellonella L. J Gen Virol 69:177–185 [CrossRef]
    [Google Scholar]
  10. Crook N. E., Spencer R. A., Payne C. C., Leisy D. J. 1985; Variation in Cydia pomonella granulosis virus isolates and physical maps of the DNA from three variants. J Gen Virol 66:2423–2430 [CrossRef]
    [Google Scholar]
  11. Federici B. A. 1997; Baculovirus pathogenesis. In The Baculoviruses pp. 33–59 Edited by Miller L. K. New York: Plenum Press; [CrossRef]
    [Google Scholar]
  12. Finney D. J. 1971 Probit Analysis Cambridge: Cambridge University Press;
    [Google Scholar]
  13. Funk C. J., Braunagel S. C., Rohrmann G. F. 1997; Baculovirus structure. In The Baculoviruses pp. 7–32 Edited by Miller L. K. New York: Plenum Press; [CrossRef]
    [Google Scholar]
  14. Graham R. I., Tyne W. I., Possee R. D., Sait S. M., Hails R. S. 2004; Genetically variable nucleopolyhedroviruses isolated from spatially separate populations of the winter moth Operophtera brumata (Lepidoptera: Geometridae) in Orkney. J Invertebr Pathol 87:29–38 [CrossRef][PubMed]
    [Google Scholar]
  15. Grant M., Bouwer G. 2009; Variation in the time-mortality responses of laboratory and field populations of Helicoverpa armigera infected with nucleopolyhedrovirus. J Appl Entomol 133:647–650 [CrossRef]
    [Google Scholar]
  16. Harris K. A., Teo C. G. 2001; Diversity of hepatitis C virus quasispecies evaluated by denaturing gradient gel electrophoresis. Clin Diagn Lab Immunol 8:62–73[PubMed]
    [Google Scholar]
  17. Herniou E. A., Jehle J. A. 2007; Baculovirus phylogeny and evolution. Curr Drug Targets 8:1043–1050 [CrossRef][PubMed]
    [Google Scholar]
  18. Hitchman R. B., Hodgson D. J., King L. A., Hails R. S., Cory J. S., Possee R. D. 2007; Host mediated selection of pathogen genotypes as a mechanism for the maintenance of baculovirus diversity in the field. J Invertebr Pathol 94:153–162 [CrossRef][PubMed]
    [Google Scholar]
  19. Hodgson D. J., Vanbergen A. J., Watt A. D., Hails R. S., Cory J. S. 2001; Phenotypic variation between naturally co-existing genotypes of a Lepidopteran baculovirus. Evol Ecol Res 3:687–701
    [Google Scholar]
  20. Hodgson D. J., Vanbergen A. J., Hartley S. E., Hails R. S., Cory J. S. 2002; Differential selection of baculovirus genotypes mediated by different species of host food plant. Ecol Lett 5:512–518 [CrossRef]
    [Google Scholar]
  21. Hughes P. R., Wood H. A. 1981; A synchronous peroral technique for the bioassay of insect viruses. J Invertebr Pathol 37:154–159 [CrossRef]
    [Google Scholar]
  22. Jehle J. A., Blissard G. W., Bonning B. C., Cory J. S., Herniou E. A., Rohrmann G. F., Theilmann D. A., Thiem S. M., Vlak J. M. 2006; On the classification and nomenclature of baculoviruses: a proposal for revision. Arch Virol 151:1257–1266 [CrossRef][PubMed]
    [Google Scholar]
  23. López-Ferber M., Simón O., Williams T., Caballero P. 2003; Defective or effective? Mutualistic interactions between virus genotypes. Proc Biol Sci 270:2249–2255 [CrossRef][PubMed]
    [Google Scholar]
  24. Magurran A. E. 1988 Ecological Diversity and its Measurement New Jersey: Princeton University Press; [CrossRef]
    [Google Scholar]
  25. Magurran A. E. 2004 Measuring Biological Diversity Oxford: Blackwell Science;
    [Google Scholar]
  26. Martin D. W., Weber P. C. 1997; DNA replication promotes high-frequency homologous recombination during Autographa californica multiple nuclear polyhedrosis virus infection. Virology 232:300–309 [CrossRef][PubMed]
    [Google Scholar]
  27. Motta F. C., Rosado A. S., Couceiro J. N. 2002; Standardization of denaturing gradient gel electrophoresis for mutant screening of influenza A (H3N2) virus samples. J Virol Methods 101:105–115 [CrossRef][PubMed]
    [Google Scholar]
  28. Parnell M., Grzywacz D., Jones K. A., Brown M., Oduor G., Ong’aro J. 2002; The strain variation and virulence of granulovirus of diamondback moth (Plutella xylostella Linnaeus, Lep., Yponomeutidae) isolated in Kenya. J Invertebr Pathol 79:192–196 [CrossRef][PubMed]
    [Google Scholar]
  29. Pearson K. 1926; On the coefficient of radical likeliness. Biometrika 18:105–117 [CrossRef]
    [Google Scholar]
  30. Pielou E. C. 1966; The measurement of diversity in different types of biological collections. J Theor Biol 13:131–144 [CrossRef]
    [Google Scholar]
  31. Shannon C. E. 1948; A mathematical theory of communication. Bell Syst Tech J 27:379–423 [CrossRef]
    [Google Scholar]
  32. Short S. M., Suttle C. A. 1999; Use of the polymerase chain reaction and denaturing gradient gel electrophoresis to study diversity in natural virus communities. Hydrobiologia 401:19–33 [CrossRef]
    [Google Scholar]
  33. Simón O., Williams T., López-Ferber M., Caballero P. 2005; Functional importance of deletion mutant genotypes in an insect nucleopolyhedrovirus population. Appl Environ Microbiol 71:4254–4262 [CrossRef][PubMed]
    [Google Scholar]
  34. Simón O., Williams T., López-Ferber M., Taulemesse J., Caballero P. 2008; Population genetic structure determines speed of kill and occlusion body production in Spodoptera frugiperda multiple nucleopolyhedrovirus. Biol Control 44:321–330 [CrossRef]
    [Google Scholar]
  35. Sun X., Chen X., Zhang Z., Wang H., Bianchi F. J. J. A., Peng H., Vlak J. M., Hu Z. 2002; Bollworm responses to release of genetically modified Helicoverpa armigera nucleopolyhedroviruses in cotton. J Invertebr Pathol 81:63–69 [CrossRef][PubMed]
    [Google Scholar]
  36. Sun X., Wang H., Sun X. C., Chen X., Peng C., Pan D., Jehle J. A., van der Werf W., Vlak J. M., Hu Z. 2004; Biological activity and field efficacy of a genetically modified Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus expressing an insect-selective toxin from a chimeric promoter. Biol Control 29:124–137 [CrossRef]
    [Google Scholar]
  37. Theilmann D. A., Blissard G. W. 2008; Baculoviruses: molecular biology of nucleopolyhedroviruses. In Encyclopedia of Virology, 3rd edn. pp. 254–265 Oxford: Academic Press; [CrossRef]
    [Google Scholar]
  38. van Oers M. M., Vlak J. M. 2007; Baculovirus genomics. Curr Drug Targets 8:1051–1068 [CrossRef][PubMed]
    [Google Scholar]
  39. White S. M., Burden J. P., Maini P. K., Hails R. S. 2012; Modelling the within-host growth of viral infections in insects. J Theor Biol 312:34–43 [CrossRef][PubMed]
    [Google Scholar]
  40. Zwart M. P., Hemerik L., Cory J. S., de Visser J. A. G. M., Bianchi F. J. J. A., Van Oers M. M., Vlak J. M., Hoekstra R. F., Van der Werf W. 2009; An experimental test of the independent action hypothesis in virus–insect pathosystems. Proc Biol Sci 276:2233–2242 [CrossRef][PubMed]
    [Google Scholar]
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