RT Journal Article SR Electronic(1) A1 Perera, Srini A1 Krell, Peter A1 Demirbag, Zihni A1 Nalçacioğlu, Remziye A1 Arif, BasilYR 2013 T1 Induction of apoptosis by the Amsacta moorei entomopoxvirus JF Journal of General Virology, VO 94 IS 8 SP 1876 OP 1887 DO https://doi.org/10.1099/vir.0.051888-0 PB Microbiology Society, SN 1465-2099, AB CF-70-B2 cells derived from the spruce budworm (Choristoneura fumiferana) undergo apoptosis when infected with Amsacta moorei entomopoxvirus (AMEV), as characterized by membrane blebbing, formation of apoptotic bodies, TdT-mediated dUTP nick-end labelling (TUNEL) staining, condensed chromatin and induction of caspase-3/7 activity. The apoptotic response was reduced when cells were infected with UV-inactivated AMEV, but not when infected in the presence of the DNA synthesis inhibitor, cytosine β-d-arabinofuranoside. Hence, only pre-DNA replication events were involved in inducing the antiviral response in CF-70-B2 cells. The virus eventually overcame the host’s antiviral response and replicated to high progeny virus titres accompanied by high levels of caspase-3/7 activity. The CF-70-B2 cells were less productive of progeny virus in comparison to LD-652, a Lymantria dispar cell line routinely used for propagation of AMEV. At late stages of infection, LD-652 cells also showed characteristics of apoptosis such as oligosomal DNA fragmentation, TUNEL staining, condensed chromatin and increased caspase-3/7 activity. Induction of apoptosis in LD-652 cells was dependent on viral DNA replication and/or late gene expression. A significantly reduced rate of infection was observed in the presence of general caspase inhibitors Q-VD-OPH and Z-VAD-FMK, indicating caspases may be involved in productive virus infection., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.051888-0