1887

Abstract

In 2009, 2589 mosquitoes were collected in northwest Italy and screened for orthobunyavirus RNA by RT-PCR. One pool of complex mosquitoes was found to be positive and a virus was isolated from that pool. The isolate was identified as Batai virus (BATV) by sequencing. Previously, BATV was detected in Italy, but limited data and no prior isolates existed. Full-length sequences of the S, M and L segments were determined for the newly isolated Italian strain. For comparison, partial sequences were also determined for the BATV strain Calovo (former Czechoslovakia, 1960). Phylogenetic analyses revealed clustering of the newly derived Italian BATV along with a recent isolate from Germany and the historic strain Calovo. To the best of our knowledge, this represents the first isolation of BATV from Italy, which confirms a broader geographical distribution of BATV in Europe than was previously verified by isolation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.051359-0
2013-06-01
2024-11-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/94/6/1242.html?itemId=/content/journal/jgv/10.1099/vir.0.051359-0&mimeType=html&fmt=ahah

References

  1. Albanese M., Di Cuonzo G., Randazzo G., Srihongse S., Tringali G. 1971; Survey for arbovirus antibodies in domestic animals of western Sicily. Ann Sclavo 13:641–647[PubMed]
    [Google Scholar]
  2. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410[PubMed] [CrossRef]
    [Google Scholar]
  3. Bárdoš V., Čupková E. 1962; The Calovo virus–the second virus isolated from mosquitoes in Czechoslovakia. J Hyg Epidemiol Microbiol Immunol 6:186–192[PubMed]
    [Google Scholar]
  4. Bárdoš V., Sluka F., Čupková E. 1969; Serological study on the medical importance of Čalovo virus. In Arboviruses of the California Complex and the Bunyamwera Group: Proceedings of the Symposium Held at Smolenice Near Bratislava October 18–21, 1966 pp. 333–335 Edited by Bárdoš V. et al. Bratislava: Slovak Academy of Sciences;
    [Google Scholar]
  5. Beaty B. J., Sundin D. R., Chandler L. J., Bishop D. H. 1985; Evolution of bunyaviruses by genome reassortment in dually infected mosquitoes (Aedes triseriatus). Science 230:548–550 [View Article][PubMed]
    [Google Scholar]
  6. Beaty B. J., Borucki M., Farfan J., White D. 1997; Arbovirus-vector interactions: determinants of arbovirus evolution. In Factors in the Emergence of Arbovirus Diseases pp. 23–35 Edited by Saluzzo J. F., Dodet B. Paris: Elsevier;
    [Google Scholar]
  7. Borucki M. K., Chandler L. J., Parker B. M., Blair C. D., Beaty B. J. 1999; Bunyavirus superinfection and segment reassortment in transovarially infected mosquitoes. J Gen Virol 80:3173–3179[PubMed]
    [Google Scholar]
  8. Bowen M. D., Trappier S. G., Sanchez A. J., Meyer R. F., Goldsmith C. S., Zaki S. R., Dunster L. M., Peters C. J., Ksiazek T. G. et al. 2001; A reassortant bunyavirus isolated from acute hemorrhagic fever cases in Kenya and Somalia. Virology 291:185–190 [View Article][PubMed]
    [Google Scholar]
  9. Briese T., Bird B., Kapoor V., Nichol S. T., Lipkin W. I. 2006; Batai and Ngari viruses: M segment reassortment and association with severe febrile disease outbreaks in East Africa. J Virol 80:5627–5630 [View Article][PubMed]
    [Google Scholar]
  10. Briese T., Kapoor V., Lipkin W. I. 2007; Natural M-segment reassortment in Potosi and Main Drain viruses: implications for the evolution of orthobunyaviruses. Arch Virol 152:2237–2247 [View Article][PubMed]
    [Google Scholar]
  11. Brummer-Korvenkontio M. 1974; Bunyamwera arbovirus supergroup in Finland, a study on Inkoo and Batai viruses. Commentationes Biologicae 76:1–52
    [Google Scholar]
  12. Calzolari M., Bonilauri P., Bellini R., Caimi M., Defilippo F., Maioli G., Albieri A., Medici A., Veronesi R. other authors 2010; Arboviral survey of mosquitoes in two northern Italian regions in 2007 and 2008. Vector Borne Zoonotic Dis 10:875–884 [View Article][PubMed]
    [Google Scholar]
  13. Cheng L. L., Rodas J. D., Schultz K. T., Christensen B. M., Yuill T. M., Israel B. A. 1999; Potential for evolution of California serogroup bunyaviruses by genome reassortment in Aedes albopictus . Am J Trop Med Hyg 60:430–438[PubMed]
    [Google Scholar]
  14. Chung S., Livingston C. W. Jr, Edwards J. F., Crandell R. W., Shope R. E., Shelton M. J., Collisson E. W. 1990; Evidence that Cache Valley virus induces congenital malformations in sheep. Vet Microbiol 21:297–307 [View Article][PubMed]
    [Google Scholar]
  15. Dunn E. F., Pritlove D. C., Elliott R. M. 1994; The S RNA genome segments of Batai, Cache Valley, Guaroa, Kairi, Lumbo, Main Drain and Northway bunyaviruses: sequence determination and analysis. J Gen Virol 75:597–608 [View Article][PubMed]
    [Google Scholar]
  16. Elliott R. M., Blakqori G. 2011; Molecular biology of orthobunyaviruses. In Bunyaviridae Molecular and Cellular Biology pp. 1–39 Edited by Plyusnin A., Elliott R. M. Norfolk: Caister Academic Press;
    [Google Scholar]
  17. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  18. Geevarghese G., Prasanna N. Y., Jacob P. G., Hanumaiah, Bhat H. R. 1994; Isolation of Batai virus from sentinel domestic pig from Kolar district in Karnataka State, India. Acta Virol 38:239–240[PubMed]
    [Google Scholar]
  19. Gerrard S. R., Li L., Barrett A. D., Nichol S. T. 2004; Ngari virus is a Bunyamwera virus reassortant that can be associated with large outbreaks of hemorrhagic fever in Africa. J Virol 78:8922–8926 [View Article][PubMed]
    [Google Scholar]
  20. Groseth A., Matsuno K., Dahlstrom E., Anzick S. L., Porcella S. F., Ebihara H. 2012; Complete genome sequencing of four geographically diverse strains of Batai virus. J Virol 86:13844–13845 [View Article][PubMed]
    [Google Scholar]
  21. Hubálek Z. 2008; Mosquito-borne viruses in Europe. Parasitol Res 103:Suppl. 129–43 [View Article][PubMed]
    [Google Scholar]
  22. Jöst H., Bialonski A., Schmetz C., Günther S., Becker N., Schmidt-Chanasit J. 2011; Isolation and phylogenetic analysis of Batai virus, Germany. Am J Trop Med Hyg 84:241–243 [View Article][PubMed]
    [Google Scholar]
  23. Karabatsos N. 1985; International Catalogue of Arboviruses, Including Certain Other Viruses of Vertebrates, 3rd edn, and the 1986–1995 Supplements to the International Catalogue. San Antonio, TX: American Society of Tropical Medicine and Hygiene..
    [Google Scholar]
  24. Kuno G., Mitchell C. J., Chang G. J., Smith G. C. 1996; Detecting bunyaviruses of the Bunyamwera and California serogroups by a PCR technique. J Clin Microbiol 34:1184–1188[PubMed]
    [Google Scholar]
  25. Lambert A. J., Lanciotti R. S. 2008; Molecular characterization of medically important viruses of the genus Orthobunyavirus . J Gen Virol 89:2580–2585 [View Article][PubMed]
    [Google Scholar]
  26. Lambert A. J., Lanciotti R. S. 2009; Consensus amplification and novel multiplex sequencing method for S segment species identification of 47 viruses of the Orthobunyavirus, Phlebovirus, and Nairovirus genera of the family Bunyaviridae . J Clin Microbiol 47:2398–2404 [View Article][PubMed]
    [Google Scholar]
  27. Nashed N. W., Olson J. G., el-Tigani A. 1993; Isolation of Batai virus (Bunyaviridae:Bunyavirus) from the blood of suspected malaria patients in Sudan. Am J Trop Med Hyg 48:676–681[PubMed]
    [Google Scholar]
  28. Singh K. R., Pavri K. M. 1966; Isolation of Chittoor virus from mosquitoes and demonstration of serological conversions in sera of domestic animals at Manjri, Poona, India. Indian J Med Res 54:220–224[PubMed]
    [Google Scholar]
  29. Sluka F. 1969; The clinical picture of the Čalovo virus infection. In Arboviruses of the California Complex and the Bunyamwera Group: Proceedings of the Symposium Held at Smolenice Near Bratislava October 18–21, 1966 pp. 337–339 Edited by Bárdoš V. et al. Bratislava: Slovak Academy of Sciences;
    [Google Scholar]
  30. Tamura K., Nei M. 1993; Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526[PubMed]
    [Google Scholar]
  31. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  32. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  33. Woodall J. P. 1969; Human infections with arboviruses of the Bunyamwera group. In Arboviruses of the California Complex and Bunyamwera Group: Proceedings of the Symposium Held at Smolenice Near Bratislava October 18–21, 1966 pp. 317–332 Edited by Bárdoš V. et al. Bratislava: Slovak Academy of Sciences;
    [Google Scholar]
  34. Yanase T., Kato T., Yamakawa M., Takayoshi K., Nakamura K., Kokuba T., Tsuda T. 2006; Genetic characterization of Batai virus indicates a genomic reassortment between orthobunyaviruses in nature. Arch Virol 151:2253–2260 [View Article][PubMed]
    [Google Scholar]
/content/journal/jgv/10.1099/vir.0.051359-0
Loading
/content/journal/jgv/10.1099/vir.0.051359-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error