RT Journal Article SR Electronic(1) A1 Mehrabadi, Mohammad A1 Hussain, Mazhar A1 Asgari, SassanYR 2013 T1 MicroRNAome of Spodoptera frugiperda cells (Sf9) and its alteration following baculovirus infection JF Journal of General Virology, VO 94 IS 6 SP 1385 OP 1397 DO https://doi.org/10.1099/vir.0.051060-0 PB Microbiology Society, SN 1465-2099, AB MicroRNAs (miRNAs) as small non-coding RNAs play important roles in many biological processes such as development, cell signalling and immune response. Studies also suggest that miRNAs are important in host–virus interactions where the host limits virus infection by differentially expressing miRNAs that target essential viral genes. Here, we identified conserved and new miRNAs from Spodoptera frugiperda cells (Sf9) using a combination of deep sequencing and bioinformatics as well as experimental approaches. S. frugiperda miRNAs share common features of miRNAs in other organisms, such as uracil (U) at the 5′ end of miRNA. The 5′ ends of the miRNAs were more conserved than the 3′ ends, revealing evolutionary protection of the seed region in miRNAs. The predominant miRNAs were found to be conserved among arthropods. The majority of homologous miRNAs were found in Bombyx mori, with 76 of the 90 identified miRNAs. We found that seed shifting and arm switching have happened in this insect's miRNAs. Expression levels of the majority of miRNAs changed following baculovirus infection. Results revealed that baculovirus infection mainly led to an overall suppression of cellular miRNAs. We found four different genes being regulated by sfr-miR-184 at the post-transcriptional level. The data presented here further support conservation of miRNAs in insects and other organisms. In addition, the results reveal a differential expression of host miRNAs upon baculovirus infection, suggesting their potential roles in host–virus interactions. Seed shifting and arm switching happened during evolution of miRNAs in different insects and caused miRNA diversification, which led to changes in the target repository of miRNAs., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.051060-0