1887

Journal of General Virology header logo

Volume 94, Issue 6

Identification of a novel polyomavirus from vervet monkeys in Zambia Free

Abstract

To examine polyomavirus (PyV) infection in wildlife, we investigated the presence of PyVs in Zambia with permission from the Zambia Wildlife Authority. We analysed 200 DNA samples from the spleens and kidneys ( = 100 each) of yellow baboons and vervet monkeys (VMs) ( = 50 each). We detected seven PyV genome fragments in 200 DNA samples using a nested broad-spectrum PCR method, and identified five full-length viral genomes using an inverse PCR method. Phylogenetic analysis of virally encoded proteins revealed that four PyVs were closely related to either African green monkey PyV or simian agent 12. Only one virus detected from a VM spleen was found to be related, with relatively low nucleotide sequence identity (74 %), to the chimpanzee PyV, which shares 48 % nucleotide sequence identity with the human Merkel cell PyV identified from Merkel cell carcinoma. The obtained entire genome of this virus was 5157 bp and had large T- and small t-antigens, and VP1 and VP2 ORFs. This virus was tentatively named vervet monkey PyV 1 (VmPyV1) as a novel PyV. Comparison with other PyVs revealed that VmPyV1, like chimpanzee PyV, had a longer VP1 ORF. To examine whether the VmPyV1 genome could produce viral proteins in cultured cells, the whole genome was transfected into HEK293T cells. We detected VP1 protein expression in the transfected HEK293T cells by immunocytochemical and immunoblot analyses. Thus, we identified a novel PyV genome from VM spleen.

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.050740-0
2013-06-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/94/6/1357.html?itemId=/content/journal/jgv/10.1099/vir.0.050740-0&mimeType=html&fmt=ahah

References

  1. Allander T., Andreasson K., Gupta S., Bjerkner A., Bogdanovic G., Persson M. A., Dalianis T., Ramqvist T., Andersson B. 2007; Identification of a third human polyomavirus. J Virol 81:4130–4136 [View Article][PubMed]
     [Google Scholar]
  2. Bergsagel D. J., Finegold M. J., Butel J. S., Kupsky W. J., Garcea R. L. 1992; DNA sequences similar to those of simian virus 40 in ependymomas and choroid plexus tumors of childhood. N Engl J Med 326:988–993 [View Article][PubMed]
     [Google Scholar]
  3. Braun L., Kalter S. S., Yakovleva L. A., Kaschula V. R., Shah K. V. 1980; Neutralizing antibodies to simian papovavirus SA12 in Old World primates in laboratory colonies: high prevalence in baboons. J Med Primatol 9:240–246[PubMed]
     [Google Scholar]
  4. Buck C. B., Phan G. Q., Raiji M. T., Murphy P. M., McDermott D. H., McBride A. A. 2012; Complete genome sequence of a tenth human polyomavirus. J Virol 86:10887 [View Article][PubMed]
     [Google Scholar]
  5. Cantalupo P., Doering A., Sullivan C. S., Pal A., Peden K. W., Lewis A. M., Pipas J. M. 2005; Complete nucleotide sequence of polyomavirus SA12. J Virol 79:13094–13104 [View Article][PubMed]
     [Google Scholar]
  6. Deuzing I., Fagrouch Z., Groenewoud M. J., Niphuis H., Kondova I., Bogers W., Verschoor E. J. 2010; Detection and characterization of two chimpanzee polyomavirus genotypes from different subspecies. Virol J 7:347 [View Article][PubMed]
     [Google Scholar]
  7. Fagrouch Z., Sarwari R., Lavergne A., Delaval M., de Thoisy B., Lacoste V., Verschoor E. J. 2012; Novel polyomaviruses in South American bats and their relationship to other members of the family Polyomaviridae . J Gen Virol 93:2652–2657 [View Article][PubMed]
     [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  9. Feng H., Shuda M., Chang Y., Moore P. S. 2008; Clonal integration of a polyomavirus in human Merkel cell carcinoma. Science 319:1096–1100 [View Article][PubMed]
     [Google Scholar]
  10. Gardner S. D., Field A. M., Coleman D. V., Hulme B. 1971; New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet 297:1253–1257 [View Article][PubMed]
     [Google Scholar]
  11. Gaynor A. M., Nissen M. D., Whiley D. M., Mackay I. M., Lambert S. B., Wu G., Brennan D. C., Storch G. A., Sloots T. P., Wang D. 2007; Identification of a novel polyomavirus from patients with acute respiratory tract infections. PLoS Pathog 3:e64 [View Article][PubMed]
     [Google Scholar]
  12. Groenewoud M. J., Fagrouch Z., van Gessel S., Niphuis H., Bulavaite A., Warren K. S., Heeney J. L., Verschoor E. J. 2010; Characterization of novel polyomaviruses from Bornean and Sumatran orang-utans. J Gen Virol 91:653–658 [View Article][PubMed]
     [Google Scholar]
  13. Halami M. Y., Dorrestein G. M., Couteel P., Heckel G., Müller H., Johne R. 2010; Whole-genome characterization of a novel polyomavirus detected in fatally diseased canary birds. J Gen Virol 91:3016–3022 [View Article][PubMed]
     [Google Scholar]
  14. Hockings K. J., Yamakoshi G., Kabasawa A., Matsuzawa T. 2010; Attacks on local persons by chimpanzees in Bossou, Republic of Guinea: long-term perspectives. Am J Primatol 72:887–896 [View Article][PubMed]
     [Google Scholar]
  15. Jiang M., Abend J. R., Johnson S. F., Imperiale M. J. 2009; The role of polyomaviruses in human disease. Virology 384:266–273 [View Article][PubMed]
     [Google Scholar]
  16. Johne R., Müller H. 2003; The genome of goose hemorrhagic polyomavirus, a new member of the proposed subgenus Avipolyomavirus . Virology 308:291–302 [View Article][PubMed]
     [Google Scholar]
  17. Johne R., Müller H. 2007; Polyomaviruses of birds: etiologic agents of inflammatory diseases in a tumor virus family. J Virol 81:11554–11559 [View Article][PubMed]
     [Google Scholar]
  18. Johne R., Enderlein D., Nieper H., Müller H. 2005; Novel polyomavirus detected in the feces of a chimpanzee by nested broad-spectrum PCR. J Virol 79:3883–3887 [View Article][PubMed]
     [Google Scholar]
  19. Kasamatsu H., Nehorayan A. 1979; Intracellular localization of viral polypeptides during simian virus 40 infection. J Virol 32:648–660[PubMed]
     [Google Scholar]
  20. Klein G., Powers A., Croce C. 2002; Association of SV40 with human tumors. Oncogene 21:1141–1149 [View Article][PubMed]
     [Google Scholar]
  21. Lednicky J. A., Garcea R. L., Bergsagel D. J., Butel J. S. 1995; Natural simian virus 40 strains are present in human choroid plexus and ependymoma tumors. Virology 212:710–717 [View Article][PubMed]
     [Google Scholar]
  22. Leendertz F. H., Scuda N., Cameron K. N., Kidega T., Zuberbühler K., Leendertz S. A., Couacy-Hymann E., Boesch C., Calvignac S., Ehlers B. 2011; African great apes are naturally infected with polyomaviruses closely related to Merkel cell polyomavirus. J Virol 85:916–924 [View Article][PubMed]
     [Google Scholar]
  23. Lim E. S., Reyes A., Antonio M., Saha D., Ikumapayi U. N., Adeyemi M., Stine O. C., Skelton R., Brennan D. C., Mkakosya R. S. 2013; Discovery of STL polyomavirus, a polyomavirus of ancestral recombinant origin that encodes a unique T antigen by alternative splicing. Virology 436:295–303[PubMed] [CrossRef]
     [Google Scholar]
  24. Orba Y., Kobayashi S., Nakamura I., Ishii A., Hang’ombe B. M., Mweene A. S., Thomas Y., Kimura T., Sawa H. 2011; Detection and characterization of a novel polyomavirus in wild rodents. J Gen Virol 92:789–795 [View Article][PubMed]
     [Google Scholar]
  25. Padgett B. L., ZuRhein G. M., Walker D. L., Eckroade R. J., Dessel B. H. 1971; Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy. Lancet 297:1257–1260 [View Article][PubMed]
     [Google Scholar]
  26. Pawlita M., Clad A., zur Hausen H. 1985; Complete DNA sequence of lymphotropic papovavirus: prototype of a new species of the polyomavirus genus. Virology 143:196–211 [View Article][PubMed]
     [Google Scholar]
  27. Pipas J. M. 2009; SV40: Cell transformation and tumorigenesis. Virology 384:294–303 [View Article][PubMed]
     [Google Scholar]
  28. Renshaw R. W., Wise A. G., Maes R. K., Dubovi E. J. 2012; Complete genome sequence of a polyomavirus isolated from horses. J Virol 86:8903 [View Article][PubMed]
     [Google Scholar]
  29. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  30. Schowalter R. M., Pastrana D. V., Pumphrey K. A., Moyer A. L., Buck C. B. 2010; Merkel cell polyomavirus and two previously unknown polyomaviruses are chronically shed from human skin. Cell Host Microbe 7:509–515 [View Article][PubMed]
     [Google Scholar]
  31. Scuda N., Hofmann J., Calvignac-Spencer S., Ruprecht K., Liman P., Kühn J., Hengel H., Ehlers B. 2011; A novel human polyomavirus closely related to the African green monkey-derived lymphotropic polyomavirus. J Virol 85:4586–4590 [View Article][PubMed]
     [Google Scholar]
  32. Shah K., Nathanson N. 1976; Human exposure to SV40: review and comment. Am J Epidemiol 103:1–12[PubMed]
     [Google Scholar]
  33. Shein H. M., Enders J. F. 1962; Multiplication and cytopathogenicity of Simian vacuolating virus 40 in cultures of human tissues. Proc Soc Exp Biol Med 109:495–500[PubMed] [CrossRef]
     [Google Scholar]
  34. Siebrasse E. A., Reyes A., Lim E. S., Zhao G., Mkakosya R. S., Manary M. J., Gordon J. I., Wang D. 2012; Identification of MW polyomavirus, a novel polyomavirus in human stool. J Virol 86:10321–10326 [View Article][PubMed]
     [Google Scholar]
  35. Soneoka Y., Cannon P. M., Ramsdale E. E., Griffiths J. C., Romano G., Kingsman S. M., Kingsman A. J. 1995; A transient three-plasmid expression system for the production of high titer retroviral vectors. Nucleic Acids Res 23:628–633 [View Article][PubMed]
     [Google Scholar]
  36. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
     [Google Scholar]
  37. van der Meijden E., Janssens R. W., Lauber C., Bouwes Bavinck J. N., Gorbalenya A. E., Feltkamp M. C. 2010; Discovery of a new human polyomavirus associated with trichodysplasia spinulosa in an immunocompromized patient. PLoS Pathog 6:e1001024 [View Article][PubMed]
     [Google Scholar]
  38. Wellehan J. F. Jr, Rivera R., Archer L. L., Benham C., Muller J. K., Colegrove K. M., Gulland F. M., St Leger J. A., Venn-Watson S. K., Nollens H. H. 2011; Characterization of California sea lion polyomavirus 1: expansion of the known host range of the Polyomaviridae to Carnivora. Infect Genet Evol 11:987–996 [View Article][PubMed]
     [Google Scholar]
  39. Yu G., Greninger A. L., Isa P., Phan T. G., Martínez M. A., de la Luz Sanchez M., Contreras J. F., Santos-Preciado J. I., Parsonnet J. other authors 2012; Discovery of a novel polyomavirus in acute diarrheal samples from children. PLoS ONE 7:e49449 [View Article][PubMed]
     [Google Scholar]
  40. Zielonka A., Verschoor E. J., Gedvilaite A., Roesler U., Müller H., Johne R. 2011; Detection of chimpanzee polyomavirus-specific antibodies in captive and wild-caught chimpanzees using yeast-expressed virus-like particles. Virus Res 155:514–519 [View Article][PubMed]
     [Google Scholar]
  41. Zinner D., Groeneveld L. F., Keller C., Roos C. 2009; Mitochondrial phylogeography of baboons (Papio spp.): indication for introgressive hybridization?. BMC Evol Biol 9:83 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.050740-0
Loading
Identification of a novel polyomavirus from vervet monkeys in Zambia
J. Gen. Virol. 94, 1357 (2013); https://doi.org/10.1099/vir.0.050740-0
/content/journal/jgv/10.1099/vir.0.050740-0
/content/journal/jgv/10.1099/vir.0.050740-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error