1887

Abstract

nucleopolyhedrovirus (BmNPV) is a highly conserved gene that is found in all of the baculovirus genomes sequenced to date and is also found in nudiviruses. To investigate the role of in virus infection , a deletion virus (vBmP95-De) was generated by homologous recombination in . Fluorescence and light microscopy and titration analysis indicated that the deletion bacmid led to a defect in production of infectious budded virus (BV). However, deletion of did not affect viral DNA replication. Electron microscopy showed that masses of aberrant tubular structures were present in cells transfected with the deletion bacmid, indicating that deletion of affected assembly of the nucleocapsid. This defect could be rescued by insertion of full-length into the polyhedrin locus of the -knockout bacmid but not the N-terminal domain of . Together, these results showed that full-length is essential for BV production and is required for nucleocapsid assembly.

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2013-07-01
2020-01-21
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