1887

Abstract

The aim of this work is to identify antigenic regions within the ORF1 protein of (TTSuV1) and (TTSuV2) that could be used as antigens to detect virus-specific antibodies following infection in pigs. Protein sequences of TTSuV ORF1 genes were analysed to predict linear antigenic epitopes. Synthesized peptides were analysed for serological reactivity with swine sera. Such an antigenic region was identified at the C terminus of the ORF1 protein of both viruses and showed serological reactivity with 78 % (TTSuV1) and 88 % (TTSuV2) of swine sera. An ELISA with an immunodominant peptide as antigen was used to examine the sera of piglets, aged 4–20 weeks, and adults. Results indicated that TTSuV1- and TTSuV2-specific antibodies were detectable at 4 weeks. Antibody titres increased from week 10 and peaked at week 20. A relatively high antibody titre persisted to adulthood.

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2013-06-01
2020-01-22
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References

  1. Aramouni M., Segalés J., Cortey M., Kekarainen T.. ( 2010;). Age-related tissue distribution of swine Torque teno sus virus 1 and 2. . Vet Microbiol 146:, 350–353. [CrossRef][PubMed]
    [Google Scholar]
  2. Bigarré L., Beven V., de Boisséson C., Grasland B., Rose N., Biagini P., Jestin A.. ( 2005;). Pig anelloviruses are highly prevalent in swine herds in France. . J Gen Virol 86:, 631–635. [CrossRef][PubMed]
    [Google Scholar]
  3. Brassard J., Gagné M. J., Houde A., Poitras E., Ward P.. ( 2010;). Development of a real-time TaqMan PCR assay for the detection of porcine and bovine Torque teno virus. . J Appl Microbiol 108:, 2191–2198.[PubMed]
    [Google Scholar]
  4. Carstens E. B.. ( 2010;). Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2009). . Arch Virol 155:, 133–146. [CrossRef][PubMed]
    [Google Scholar]
  5. Cong M. E., Nichols B., Dou X. G., Spelbring J. E., Krawczynski K., Fields H. A., Khudyakov Y. E.. ( 2000;). Related TT viruses in chimpanzees. . Virology 274:, 343–355. [CrossRef][PubMed]
    [Google Scholar]
  6. Cornelissen-Keijsers V., Jiménez-Melsió A., Sonnemans D., Cortey M., Segalés J., van den Born E., Kekarainen T.. ( 2012;). Discovery of a novel Torque teno sus virus species: genetic characterization, epidemiological assessment and disease association. . J Gen Virol 93:, 2682–2691. [CrossRef][PubMed]
    [Google Scholar]
  7. Cortey M., Pileri E., Segalés J., Kekarainen T.. ( 2012;). Globalisation and global trade influence molecular viral population genetics of Torque Teno Sus Viruses 1 and 2 in pigs. . Vet Microbiol 156:, 81–87. [CrossRef][PubMed]
    [Google Scholar]
  8. Emini E. A., Hughes J. V., Perlow D. S., Boger J.. ( 1985;). Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide. . J Virol 55:, 836–839.[PubMed]
    [Google Scholar]
  9. Gallei A., Pesch S., Esking W. S., Keller C., Ohlinger V. F.. ( 2010;). Porcine Torque teno virus: determination of viral genomic loads by genogroup-specific multiplex rt-PCR, detection of frequent multiple infections with genogroups 1 or 2, and establishment of viral full-length sequences. . Vet Microbiol 143:, 202–212. [CrossRef][PubMed]
    [Google Scholar]
  10. Huang Y. W., Dryman B. A., Harrall K. K., Vaughn E. M., Roof M. B., Meng X. J.. ( 2010;). Development of SYBR green-based real-time PCR and duplex nested PCR assays for quantitation and differential detection of species- or type-specific porcine Torque teno viruses. . J Virol Methods 170:, 140–146. [CrossRef][PubMed]
    [Google Scholar]
  11. Huang Y. W., Harrall K. K., Dryman B. A., Beach N. M., Kenney S. P., Opriessnig T., Vaughn E. M., Roof M. B., Meng X. J.. ( 2011;). Expression of the putative ORF1 capsid protein of Torque teno sus virus 2 (TTSuV2) and development of Western blot and ELISA serodiagnostic assays: correlation between TTSuV2 viral load and IgG antibody level in pigs. . Virus Res 158:, 79–88. [CrossRef][PubMed]
    [Google Scholar]
  12. Huang Y. W., Harrall K. K., Dryman B. A., Opriessnig T., Vaughn E. M., Roof M. B., Meng X. J.. ( 2012;). Serological profile of torque teno sus virus species 1 (TTSuV1) in pigs and antigenic relationships between two TTSuV1 genotypes (1a and 1b), between two species (TTSuV1 and -2), and between porcine and human anelloviruses. . J Virol 86:, 10628–10639. [CrossRef][PubMed]
    [Google Scholar]
  13. Jarosova V., Pogranichniy R., Celer V.. ( 2011;). Prevalence and age distribution of porcine torque teno sus virus (TTSuV) in the Czech Republic. . Folia Microbiol (Praha) 56:, 90–94. [CrossRef][PubMed]
    [Google Scholar]
  14. Kekarainen T., Segalés J.. ( 2009;). Torque teno virus infection in the pig and its potential role as a model of human infection. . Vet J 180:, 163–168. [CrossRef][PubMed]
    [Google Scholar]
  15. Kekarainen T., Sibila M., Segalés J.. ( 2006;). Prevalence of swine Torque teno virus in post-weaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs in Spain. . J Gen Virol 87:, 833–837. [CrossRef][PubMed]
    [Google Scholar]
  16. Kekarainen T., López-Soria S., Segalés J.. ( 2007;). Detection of swine Torque teno virus genogroups 1 and 2 in boar sera and semen. . Theriogenology 68:, 966–971. [CrossRef][PubMed]
    [Google Scholar]
  17. Kolaskar A. S., Tongaonkar P. C.. ( 1990;). A semi-empirical method for prediction of antigenic determinants on protein antigens. . FEBS Lett 276:, 172–174. [CrossRef][PubMed]
    [Google Scholar]
  18. Larsen J. E., Lund O., Nielsen M.. ( 2006;). Improved method for predicting linear B-cell epitopes. . Immunome Res 2:, 2. [CrossRef][PubMed]
    [Google Scholar]
  19. Leary T. P., Erker J. C., Chalmers M. L., Desai S. M., Mushahwar I. K.. ( 1999;). Improved detection systems for TT virus reveal high prevalence in humans, non-human primates and farm animals. . J Gen Virol 80:, 2115–2120.[PubMed]
    [Google Scholar]
  20. Martelli F., Caprioli A., Di Bartolo I., Cibin V., Pezzotti G., Ruggeri F. M., Ostanello F.. ( 2006;). Detection of swine torque teno virus in Italian pig herds. . J Vet Med B Infect Dis Vet Public Health 53:, 234–238. [CrossRef][PubMed]
    [Google Scholar]
  21. Martínez-Guinó L., Kekarainen T., Segalés J.. ( 2009;). Evidence of Torque teno virus (TTV) vertical transmission in swine. . Theriogenology 71:, 1390–1395. [CrossRef][PubMed]
    [Google Scholar]
  22. McKeown N. E., Fenaux M., Halbur P. G., Meng X. J.. ( 2004;). Molecular characterization of porcine TT virus, an orphan virus, in pigs from six different countries. . Vet Microbiol 104:, 113–117. [CrossRef][PubMed]
    [Google Scholar]
  23. Nieto D., Aramouni M., Sibila M., Fraile L., Kekarainen T., Segalés J.. ( 2012;). Lack of effect of piglet vaccination against Porcine circovirus type 2 (PCV2) on serum viral loads of Torque teno sus virus 2 (TTSuV2). . Vet Microbiol 157:, 8–12. [CrossRef][PubMed]
    [Google Scholar]
  24. Nishizawa T., Okamoto H., Konishi K., Yoshizawa H., Miyakawa Y., Mayumi M.. ( 1997;). A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown etiology. . Biochem Biophys Res Commun 241:, 92–97. [CrossRef][PubMed]
    [Google Scholar]
  25. Okamoto H., Nishizawa T., Takahashi M., Tawara A., Peng Y. H., Kishimoto J., Wang Y.. ( 2001;). Genomic and evolutionary characterization of TT virus (TTV) in tupaias and comparison with species-specific TTVs in humans and non-human primates. . J Gen Virol 82:, 2041–2050.[PubMed]
    [Google Scholar]
  26. Okamoto H., Takahashi M., Nishizawa T., Tawara A., Fukai K., Muramatsu U., Naito Y., Yoshikawa A.. ( 2002;). Genomic characterization of TT viruses (TTVs) in pigs, cats and dogs and their relatedness with species-specific TTVs in primates and tupaias. . J Gen Virol 83:, 1291–1297.[PubMed]
    [Google Scholar]
  27. Parker J. M., Guo D., Hodges R. S.. ( 1986;). New hydrophilicity scale derived from high-performance liquid chromatography peptide retention data: correlation of predicted surface residues with antigenicity and X-ray-derived accessible sites. . Biochemistry 25:, 5425–5432. [CrossRef][PubMed]
    [Google Scholar]
  28. Pozzuto T., Mueller B., Meehan B., Ringler S. S., McIntosh K. A., Ellis J. A., Mankertz A., Krakowka S.. ( 2009;). In utero transmission of porcine torque teno viruses. . Vet Microbiol 137:, 375–379. [CrossRef][PubMed]
    [Google Scholar]
  29. Sibila M., Martínez-Guinó L., Huerta E., Llorens A., Mora M., Grau-Roma L., Kekarainen T., Segalés J.. ( 2009a;). Swine torque teno virus (TTV) infection and excretion dynamics in conventional pig farms. . Vet Microbiol 139:, 213–218. [CrossRef][PubMed]
    [Google Scholar]
  30. Sibila M., Martínez-Guinó L., Huerta E., Mora M., Grau-Roma L., Kekarainen T., Segalés J.. ( 2009b;). Torque teno virus (TTV) infection in sows and suckling piglets. . Vet Microbiol 137:, 354–358. [CrossRef][PubMed]
    [Google Scholar]
  31. Taira O., Ogawa H., Nagao A., Tuchiya K., Nunoya T., Ueda S.. ( 2009;). Prevalence of swine Torque teno virus genogroups 1 and 2 in Japanese swine with suspected post-weaning multisystemic wasting syndrome and porcine respiratory disease complex. . Vet Microbiol 139:, 347–350. [CrossRef][PubMed]
    [Google Scholar]
  32. Verschoor E. J., Langenhuijzen S., Heeney J. L.. ( 1999;). TT viruses (TTV) of non-human primates and their relationship to the human TTV genotypes. . J Gen Virol 80:, 2491–2499.[PubMed]
    [Google Scholar]
  33. Xiao C. T., Giménez-Lirola L., Huang Y. W., Meng X. J., Halbur P. G., Opriessnig T.. ( 2012;). The prevalence of Torque teno sus virus (TTSuV) is common and increases with the age of growing pigs in the United States. . J Virol Methods 183:, 40–44. [CrossRef][PubMed]
    [Google Scholar]
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