1887

Abstract

A 108 bp sequence has been identified in the tomato golden mosaic virus-yellow vein (yvTGMV) B component that is necessary and sufficient for AL2-mediated activation of the promoter. The sequence appears to have a bipartite arrangement, with elements located between −144 to −77 and −59 to −36 from the transcription start site, with both being required for activation by AL2. These sequences are located upstream of a TATA box and bind nuclear proteins from spinach, tomato and . These sequences are also capable of binding PPD2, which has been shown previously to interact with the yvTGMV coat protein () promoter. We have identified two putative transcription factor-binding sites (CCAAT and GTGANTG10) that are conserved in sequences necessary for activation of the yvTGMV , as well as the yvTGMV and cabbage leaf curl virus (CabLCV) promoters, which are all activated by AL2. The yvTGMV promoter exhibits AL2-independent expression in vascular tissue, similar to the yvTGMV, CabLCV and spinach curly top virus promoters. Together, this further confirms a common regulatory mechanism for AL2-mediated activation of bipartite begomovirus promoters.

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2013-06-01
2019-10-16
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