An insect cell line derived from the small brown planthopper supports replication of rice stripe virus, a tenuivirus Free

Abstract

A cell line from the small brown planthopper (SBPH; ) was established to study replication of rice stripe virus (RSV), a tenuivirus. The SBPH cell line, which had been subcultured through 30 passages, formed monolayers of epithelial-like cells. Inoculation of cultured vector cells with RSV resulted in a persistent infection. During viral infection in the SBPH cell line, the viral non-structural protein NS3 co-localized with the filamentous ribonucleoprotein particles of RSV, as revealed by electron and confocal microscopy. The knockdown of NS3 expression due to RNA interference induced by synthesized double-stranded RNAs from the NS3 gene significantly inhibited viral infection in the SBPH cell line. These results demonstrated that NS3 of RSV might be involved in viral replication or assembly. The persistent infection of the SBPH cell line by RSV will enable a better understanding of the complex relationship between RSV and its insect vector.

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2013-06-01
2024-03-28
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References

  1. Ammar D., Tsai C. W., Whitfield A. E., Redinbaugh M. G., Hogenhout S. A. 2009; Cellular and molecular aspects of rhabdovirus interactions with insect and plant hosts. Annu Rev Entomol 54:447–468 [View Article][PubMed]
    [Google Scholar]
  2. Chen Q., Chen H., Mao Q., Liu Q., Shimizu T., Uehara-Ichiki T., Wu Z., Xie L., Omura T., Wei T. 2012; Tubular structure induced by a plant virus facilitates viral spread in its vector insect. PLoS Pathog 8:e1003032 [View Article][PubMed]
    [Google Scholar]
  3. Creamer R. 1993; Invertebrate tissue culture as a tool to study insect transmission of plant viruses. In Vitro Cell Dev Biol 29A:284–288 [View Article]
    [Google Scholar]
  4. Falk B. W., Tsai J. H. 1998; Biology and molecular biology of viruses in the genus Tenuivirus. Annu Rev Phytopathol 36:139–163 [View Article][PubMed]
    [Google Scholar]
  5. Fire A., Xu S., Montgomery M. K., Kostas S. A., Driver S. E., Mello C. C. 1998; Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. . Nature 391:806–811 [View Article][PubMed]
    [Google Scholar]
  6. Hogenhout S. A., Ammar D., Whitfield A. E., Redinbaugh M. G. 2008; Insect vector interactions with persistently transmitted viruses. Annu Rev Phytopathol 46:327–359 [View Article][PubMed]
    [Google Scholar]
  7. Jia D., Chen H., Zheng A., Chen Q., Liu Q., Xie L., Wu Z., Wei T. 2012; Development of an insect vector cell culture and RNA interference system to investigate the functional role of fijivirus replication protein. J Virol 86:5800–5807 [View Article][PubMed]
    [Google Scholar]
  8. Kimura I., Omura T. 1988; Leafhopper cell cultures as a means for phytoreovirus research. Adv Dis Vector Res 5:111–135
    [Google Scholar]
  9. Mitsuhashi J. 1969; Preliminary report on the primary culture of smaller brown planthopper cell in vitro (Hemiptera: Delphacidae). Appl Entomol Zool 4:151–153
    [Google Scholar]
  10. Mitsuhashi J. 2001; Development of highly nutritive culture media. In Vitro Cell Dev Biol Anim 37:330–337[PubMed] [CrossRef]
    [Google Scholar]
  11. Omura T., Kimura I. 1994; Leafhopper cell culture for virus research. In Arthropod cell culture systems pp. 91–107 Edited by Maramorosch K., McIntosh A. H. Philadelphia, Pa.: CRC Press;
    [Google Scholar]
  12. Suzuki Y., Fuji S., Takahashi Y., Kojima M. 1992; Immunogold localization of Rice stripe virus particles antigens in thin sections of insect host cells. Ann Phytopath Soc Japan 58:480–484 [View Article]
    [Google Scholar]
  13. Takahashi Y., Omura T., Shohara K., Tsuchizaki T. 1991; Comparison of four serological methods for practical detection of ten viruses of rice in plants and insects. Plant Dis 75:458–461 [View Article]
    [Google Scholar]
  14. Toriyama S. 1986; Rice stripe virus: prototype of a new group of viruses that replicate in plants and insects. Microbiol Sci 3:347–351[PubMed]
    [Google Scholar]
  15. Wei T., Kikuchi A., Moriyasu Y., Suzuki N., Shimizu T., Hagiwara K., Chen H., Takahashi M., Ichiki-Uehara T., Omura T. 2006; The spread of Rice dwarf virus among cells of its insect vector exploits virus-induced tubular structures. J Virol 80:8593–8602 [View Article][PubMed]
    [Google Scholar]
  16. Wei T., Chen H., Ichiki-Uehara T., Hibino H., Omura T. 2007; Entry of Rice dwarf virus into cultured cells of its insect vector involves clathrin-mediated endocytosis. J Virol 81:7811–7815 [View Article][PubMed]
    [Google Scholar]
  17. Wei T. Y., Yang J. G., Liao F. R., Gao F. L., Lu L. M., Zhang X. T., Li F., Wu Z. J., Lin Q. Y. other authors 2009; Genetic diversity and population structure of rice stripe virus in China. J Gen Virol 90:1025–1034 [View Article][PubMed]
    [Google Scholar]
  18. Xiao D., Li W., Wei T., Wu Z., Xie L. 2010; Advances in the studies of Rice stripe virus. . Front Agric China 4:287–292 [View Article]
    [Google Scholar]
  19. Xiong R., Wu J., Zhou Y., Zhou X. 2009; Characterization and subcellular localization of an RNA silencing suppressor encoded by Rice stripe tenuivirus. . Virology 387:29–40 [View Article][PubMed]
    [Google Scholar]
  20. Yamada K., Tokumitsu T., Shikata E. 1970; Embryonic tissue culture of Laodelphax striatellus Fallen. Jap J Appl Entomol Zool 14:79–84 [View Article]
    [Google Scholar]
  21. Zhang K. Y., Xiong R. Y., Wu J. X., Zhou X. P., Zhou Y. J. 2008; Detection of the proteins encoded by Rice stripe virus in Laodelphax striatellus Fallén and interactions in vitro between CP and the four proteins. Sci Agricult Sinica 41:4063–4068
    [Google Scholar]
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