A cell line from the small brown planthopper (SBPH; Laodelphax striatellus) was established to study replication of rice stripe virus (RSV), a tenuivirus. The SBPH cell line, which had been subcultured through 30 passages, formed monolayers of epithelial-like cells. Inoculation of cultured vector cells with RSV resulted in a persistent infection. During viral infection in the SBPH cell line, the viral non-structural protein NS3 co-localized with the filamentous ribonucleoprotein particles of RSV, as revealed by electron and confocal microscopy. The knockdown of NS3 expression due to RNA interference induced by synthesized double-stranded RNAs from the NS3 gene significantly inhibited viral infection in the SBPH cell line. These results demonstrated that NS3 of RSV might be involved in viral replication or assembly. The persistent infection of the SBPH cell line by RSV will enable a better understanding of the complex relationship between RSV and its insect vector.
AmmarD.,
TsaiC. W.,
WhitfieldA. E.,
RedinbaughM. G.,
HogenhoutS. A.2009; Cellular and molecular aspects of rhabdovirus interactions with insect and plant hosts. Annu Rev Entomol 54:447–468 [View Article][PubMed]
FireA.,
XuS.,
MontgomeryM. K.,
KostasS. A.,
DriverS. E.,
MelloC. C.1998; Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.
. Nature 391:806–811 [View Article][PubMed]
HogenhoutS. A.,
AmmarD.,
WhitfieldA. E.,
RedinbaughM. G.2008; Insect vector interactions with persistently transmitted viruses. Annu Rev Phytopathol 46:327–359 [View Article][PubMed]
JiaD.,
ChenH.,
ZhengA.,
ChenQ.,
LiuQ.,
XieL.,
WuZ.,
WeiT.2012; Development of an insect vector cell culture and RNA interference system to investigate the functional role of fijivirus replication protein. J Virol 86:5800–5807 [View Article][PubMed]
MitsuhashiJ.1969; Preliminary report on the primary culture of smaller brown planthopper cell in vitro (Hemiptera: Delphacidae). Appl Entomol Zool 4:151–153
OmuraT.,
KimuraI.1994; Leafhopper cell culture for virus research. In Arthropod cell culture systems pp. 91–107 Edited by
MaramoroschK.,
McIntoshA. H.
Philadelphia, Pa.: CRC Press;
TakahashiY.,
OmuraT.,
ShoharaK.,
TsuchizakiT.1991; Comparison of four serological methods for practical detection of ten viruses of rice in plants and insects. Plant Dis 75:458–461 [View Article]
WeiT. Y.,
YangJ. G.,
LiaoF. R.,
GaoF. L.,
LuL. M.,
ZhangX. T.,
LiF.,
WuZ. J.,
LinQ. Y.other authors2009; Genetic diversity and population structure of rice stripe virus in China. J Gen Virol 90:1025–1034 [View Article][PubMed]
ZhangK. Y.,
XiongR. Y.,
WuJ. X.,
ZhouX. P.,
ZhouY. J.2008; Detection of the proteins encoded by Rice stripe virus in Laodelphax striatellus Fallén and interactions in vitro between CP and the four proteins. Sci Agricult Sinica 41:4063–4068