1887

Abstract

Many astrovirus (AstV) species are associated with enteric disease, although extraintestinal manifestations in mammalian and avian hosts have also been described. In this study, the prevalence rates of porcine AstV types 1–5 (PAstV1–PAstV5) were investigated using faecal samples from 509 pigs of which 488 (95.9 %) came from farms with a history of diarrhoea. All of the five known PAstV types were found to circulate in pigs in the USA, and co-infection of a single pig with two or more PAstV types was frequently observed. A high overall prevalence of 64.0 % (326/509) of PAstV RNA-positive samples was detected, with 97.2 % (317/326) of the PAstV RNA-positive pigs infected with PAstV4. Further genomic sequencing and characterization of the selected isolates revealed low sequence identities (49.2–89.0 %) with known PAstV strains, indicating novel types or genotypes of PAstV2, PAstV4 and PAstV5. Some new features of the genomes of the PAstVs were also discovered. The first complete genome of a PAstV3 isolate was obtained and showed identities of 50.5–55.3 % with mink AstV and the novel human AstVs compared with 38.4–42.7 % with other PAstV types. Phylogenetic analysis revealed that PAstV1, PAstV2 and PAstV3 were more closely related to AstVs from humans and other animals than to each other, indicating past cross-species transmission and the zoonotic potential of these PAstVs.

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2013-03-01
2019-12-14
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References

  1. Biđin M., Lojkić I., Tišljar M., Biđin Z., Majnarić D.. ( 2012;). Astroviruses associated with stunting and pre-hatching mortality in duck and goose embryos. . Avian Pathol 41:, 91–97. [CrossRef][PubMed]
    [Google Scholar]
  2. Blomström A. L., Widén F., Hammer A. S., Belák S., Berg M.. ( 2010;). Detection of a novel astrovirus in brain tissue of mink suffering from shaking mink syndrome by use of viral metagenomics. . J Clin Microbiol 48:, 4392–4396. [CrossRef][PubMed]
    [Google Scholar]
  3. Bosch A., Guix S., Krishna N. K., Méndez E., Monroe S. S., Pantin-Jackwood M., Schultz-Cherry S.. ( 2011;). Family Astroviridae. . In Virus Taxonomy: Classification and Nomenclature of Viruses (Ninth Report of the International Committee on the Taxonomy of Viruses), pp. 953–959. Edited by King A. M. Q., Lefkowitz E., Adams M. J., Carstens E. B... New York:: Elsevier Academic Press;.
    [Google Scholar]
  4. Bridger J. C.. ( 1980;). Detection by electron microscopy of caliciviruses, astroviruses and rotavirus-like particles in the faeces of piglets with diarrhoea. . Vet Rec 107:, 532–533.[PubMed]
    [Google Scholar]
  5. Chu D. K., Poon L. L., Guan Y., Peiris J. S.. ( 2008;). Novel astroviruses in insectivorous bats. . J Virol 82:, 9107–9114. [CrossRef][PubMed]
    [Google Scholar]
  6. De Benedictis P., Schultz-Cherry S., Burnham A., Cattoli G.. ( 2011;). Astrovirus infections in humans and animals – molecular biology, genetic diversity, and interspecies transmissions. . Infect Genet Evol 11:, 1529–1544. [CrossRef][PubMed]
    [Google Scholar]
  7. Finkbeiner S. R., Kirkwood C. D., Wang D.. ( 2008;). Complete genome sequence of a highly divergent astrovirus isolated from a child with acute diarrhea. . Virol J 5:, 117. [CrossRef][PubMed]
    [Google Scholar]
  8. Finkbeiner S. R., Li Y., Ruone S., Conrardy C., Gregoricus N., Toney D., Virgin H. W., Anderson L. J., Vinjé J.. & other authors ( 2009;). Identification of a novel astrovirus (astrovirus VA1) associated with an outbreak of acute gastroenteritis. . J Virol 83:, 10836–10839. [CrossRef][PubMed]
    [Google Scholar]
  9. Gallimore C. I., Taylor C., Gennery A. R., Cant A. J., Galloway A., Lewis D., Gray J. J.. ( 2005;). Use of a heminested reverse transcriptase PCR assay for detection of astrovirus in environmental swabs from an outbreak of gastroenteritis in a pediatric primary immunodeficiency unit. . J Clin Microbiol 43:, 3890–3894. [CrossRef][PubMed]
    [Google Scholar]
  10. Indik S., Valícek L., Smíd B., Dvoráková H., Rodák L.. ( 2006;). Isolation and partial characterization of a novel porcine astrovirus. . Vet Microbiol 117:, 276–283. [CrossRef][PubMed]
    [Google Scholar]
  11. Jiang B., Monroe S. S., Koonin E. V., Stine S. E., Glass R. I.. ( 1993;). RNA sequence of astrovirus: distinctive genomic organization and a putative retrovirus-like ribosomal frameshifting signal that directs the viral replicase synthesis. . Proc Natl Acad Sci U S A 90:, 10539–10543. [CrossRef][PubMed]
    [Google Scholar]
  12. Jonassen C. M., Jonassen T. O., Grinde B.. ( 1998;). A common RNA motif in the 3′ end of the genomes of astroviruses, avian infectious bronchitis virus and an equine rhinovirus. . J Gen Virol 79:, 715–718.[PubMed]
    [Google Scholar]
  13. Jonassen C. M., Jonassen T. O., Saif Y. M., Snodgrass D. R., Ushijima H., Shimizu M., Grinde B.. ( 2001;). Comparison of capsid sequences from human and animal astroviruses. . J Gen Virol 82:, 1061–1067.[PubMed]
    [Google Scholar]
  14. Kamer G., Argos P.. ( 1984;). Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses. . Nucleic Acids Res 12:, 7269–7282. [CrossRef][PubMed]
    [Google Scholar]
  15. Kozak M.. ( 1991;). Structural features in eukaryotic mRNAs that modulate the initiation of translation. . J Biol Chem 266:, 19867–19870.[PubMed]
    [Google Scholar]
  16. Lan D., Ji W., Shan T., Cui L., Yang Z., Yuan C., Hua X.. ( 2011;). Molecular characterization of a porcine astrovirus strain in China. . Arch Virol 156:, 1869–1875. [CrossRef][PubMed]
    [Google Scholar]
  17. Laurin M. A., Dastor M., L’Homme Y.. ( 2011;). Detection and genetic characterization of a novel pig astrovirus: relationship to other astroviruses. . Arch Virol 156:, 2095–2099. [CrossRef][PubMed]
    [Google Scholar]
  18. Lee M.-H., Jeoung H.-Y., Park H.-R., Lim J.-A., Song J.-Y., An D.-J.. ( 2012;). Phylogenetic analysis of porcine astrovirus in domestic pigs and wild boars in South Korea. . Virus Genes [Epub ahead of print]. [CrossRef][PubMed]
    [Google Scholar]
  19. Lewis D. C., Lightfoot N. F., Cubitt W. D., Wilson S. A.. ( 1989;). Outbreaks of astrovirus type 1 and rotavirus gastroenteritis in a geriatric in-patient population. . J Hosp Infect 14:, 9–14. [CrossRef][PubMed]
    [Google Scholar]
  20. Lewis T. L., Greenberg H. B., Herrmann J. E., Smith L. S., Matsui S. M.. ( 1994;). Analysis of astrovirus serotype 1 RNA, identification of the viral RNA-dependent RNA polymerase motif, and expression of a viral structural protein. . J Virol 68:, 77–83.[PubMed]
    [Google Scholar]
  21. Lukashov V. V., Goudsmit J.. ( 2002;). Evolutionary relationships among Astroviridae. . J Gen Virol 83:, 1397–1405.[PubMed]
    [Google Scholar]
  22. Luo Z., Roi S., Dastor M., Gallice E., Laurin M. A., L’Homme Y.. ( 2011;). Multiple novel and prevalent astroviruses in pigs. . Vet Microbiol 149:, 316–323. [CrossRef][PubMed]
    [Google Scholar]
  23. Madeley C. R., Cosgrove B. P.. ( 1975;). Letter: 28 nm particles in faeces in infantile gastroenteritis. . Lancet 306:, 451–452. [CrossRef][PubMed]
    [Google Scholar]
  24. Martella V., Moschidou P., Pinto P., Catella C., Desario C., Larocca V., Circella E., Bànyai K., Lavazza A.. & other authors ( 2011;). Astroviruses in rabbits. . Emerg Infect Dis 17:, 2287–2293. [CrossRef][PubMed]
    [Google Scholar]
  25. Mendéz E., Arias C.. ( 2007;). Astroviruses. . In Fields Virology, , 5th edn., pp. 981–1000. Edited by Knipe D., Howley P... Philadelphia, PA:: Lippincott Williams & Wilkins;.
    [Google Scholar]
  26. Mor S. K., Chander Y., Marthaler D., Patnayak D. P., Goyal S. M.. ( 2012;). Detection and molecular characterization of porcine astrovirus strains associated with swine diarrhea. . J Vet Diagn Invest 24:, 1064–1067. [CrossRef][PubMed]
    [Google Scholar]
  27. Poch O., Sauvaget I., Delarue M., Tordo N.. ( 1989;). Identification of four conserved motifs among the RNA-dependent polymerase encoding elements. . EMBO J 8:, 3867–3874.[PubMed]
    [Google Scholar]
  28. Quan P.-L., Wagner T. A., Briese T., Torgerson T. R., Hornig M., Tashmukhamedova A., Firth C., Palacios G., Baisre-De-Leon A.. & other authors ( 2010;). Astrovirus encephalitis in boy with X-linked agammaglobulinemia. . Emerg Infect Dis 16:, 918–925. [CrossRef][PubMed]
    [Google Scholar]
  29. Reuter G., Pankovics P., Boros A.. ( 2011;). Identification of a novel astrovirus in a domestic pig in Hungary. . Arch Virol 156:, 125–128. [CrossRef][PubMed]
    [Google Scholar]
  30. Reuter G., Nemes C., Boros A., Kapusinszky B., Delwart E., Pankovics P.. ( 2012;). Astrovirus in wild boars (Sus scrofa) in Hungary. . Arch Virol 157:, 1143–1147. [CrossRef][PubMed]
    [Google Scholar]
  31. Shan T., Li L., Simmonds P., Wang C., Moeser A., Delwart E.. ( 2011;). The fecal virome of pigs on a high-density farm. . J Virol 85:, 11697–11708. [CrossRef][PubMed]
    [Google Scholar]
  32. Shimizu M., Shirai J., Narita M., Yamane T.. ( 1990;). Cytopathic astrovirus isolated from porcine acute gastroenteritis in an established cell line derived from porcine embryonic kidney. . J Clin Microbiol 28:, 201–206.[PubMed]
    [Google Scholar]
  33. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  34. Tse H., Chan W.-M., Tsoi H.-W., Fan R. Y., Lau C. C., Lau S. K., Woo P. C., Yuen K.-Y.. ( 2011;). Rediscovery and genomic characterization of bovine astroviruses. . J Gen Virol 92:, 1888–1898. [CrossRef][PubMed]
    [Google Scholar]
  35. Ulloa J. C., Gutiérrez M. F.. ( 2010;). Genomic analysis of two ORF2 segments of new porcine astrovirus isolates and their close relationship with human astroviruses. . Can J Microbiol 56:, 569–577. [CrossRef][PubMed]
    [Google Scholar]
  36. Wang Q.-H., Kakizawa J., Wen L.-Y., Shimizu M., Nishio O., Fang Z.-Y., Ushijima H.. ( 2001;). Genetic analysis of the capsid region of astroviruses. . J Med Virol 64:, 245–255. [CrossRef][PubMed]
    [Google Scholar]
  37. Wunderli W., Meerbach A., Güngör T., Berger C., Greiner O., Caduff R., Trkola A., Bossart W., Gerlach D.. & other authors ( 2011;). Astrovirus infection in hospitalized infants with severe combined immunodeficiency after allogeneic hematopoietic stem cell transplantation. . PLoS ONE 6:, e27483. [CrossRef][PubMed]
    [Google Scholar]
  38. Xiao C.-T., Giménez-Lirola L., Huang Y.-W., Meng X. J., Halbur P. G., Opriessnig T.. ( 2012;). The prevalence of Torque teno sus virus (TTSuV) is common and increases with the age of growing pigs in the United States. . J Virol Methods 183:, 40–44. [CrossRef][PubMed]
    [Google Scholar]
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