RT Journal Article SR Electronic(1) A1 Huang, Yong A1 Mi, Zhiqiang A1 Zhuang, Lu A1 Ma, Maijuan A1 An, Xiaoping A1 Liu, Wei A1 Cao, Wuchun A1 Tong, YigangYR 2013 T1 Presence of entomobirnaviruses in Chinese mosquitoes in the absence of Dengue virus co‐infection JF Journal of General Virology, VO 94 IS 3 SP 663 OP 667 DO https://doi.org/10.1099/vir.0.048231-0 PB Microbiology Society, SN 1465-2099, AB Birnaviruses, including the genus Entomobirnavirus, are socio-economically important viruses. Currently, only Drosophila X virus has been formally assigned to the genus Entomobirnavirus, but two more viruses were recently isolated, Espirito Santo virus (ESV) and Culex Y virus. The host mosquito has been reported to carry many viruses, but seldom entomobirnaviruses. To discover potential pathogens in mosquitoes, we exploited small-RNAs high-throughput sequencing of three mosquito species caught in South China. A virus that genetically likes entomobirnavirus, Mosquito X virus (MXV), was identified from Anopheles sinensis and was 97 % identical to ESV, which co-infects with Dengue virus (DENV). However, the absence of DENV in the A. sinensis suggested the independence of MXV infection from dengue co-infection. Our discovery complements prior research on entomobirnaviruses and proved that MXV may be widespread in mosquitoes on different continents. This work also highlights the applying of high-throughput sequencing of small RNAs to survey viruses carried by insect vectors., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.048231-0