%0 Journal Article %A Huang, Yong %A Mi, Zhiqiang %A Zhuang, Lu %A Ma, Maijuan %A An, Xiaoping %A Liu, Wei %A Cao, Wuchun %A Tong, Yigang %T Presence of entomobirnaviruses in Chinese mosquitoes in the absence of Dengue virus co‐infection %D 2013 %J Journal of General Virology, %V 94 %N 3 %P 663-667 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.048231-0 %I Microbiology Society, %X Birnaviruses, including the genus Entomobirnavirus, are socio-economically important viruses. Currently, only Drosophila X virus has been formally assigned to the genus Entomobirnavirus, but two more viruses were recently isolated, Espirito Santo virus (ESV) and Culex Y virus. The host mosquito has been reported to carry many viruses, but seldom entomobirnaviruses. To discover potential pathogens in mosquitoes, we exploited small-RNAs high-throughput sequencing of three mosquito species caught in South China. A virus that genetically likes entomobirnavirus, Mosquito X virus (MXV), was identified from Anopheles sinensis and was 97 % identical to ESV, which co-infects with Dengue virus (DENV). However, the absence of DENV in the A. sinensis suggested the independence of MXV infection from dengue co-infection. Our discovery complements prior research on entomobirnaviruses and proved that MXV may be widespread in mosquitoes on different continents. This work also highlights the applying of high-throughput sequencing of small RNAs to survey viruses carried by insect vectors. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.048231-0